Effects of Mancozeb and Metribuzin on in vitro proliferative responses and oxidative stress of human and rat spleen lymphocytes stimulated by mitogens

In vitro effects of oil's fatty acids on T cell function in gestational diabetic pregnant women and their newborns 油类脂肪酸在体外对妊娠期糖尿病孕妇及其新生儿的T细胞功能的影响

“…Peripheral lymphocytes were separated from venous blood by Histopaque 1077 (Sigma Aldrich), as previously reported . The cells were washed and suspended in complete RPMI 1640 medium with or without mitogen, Concanavalin, ConA (Sigma Aldrich).…”

“…The viable cell number was determined by counting trypan blue unstained cells in a hemocytometer and was over 80% for all cultures. The proliferation of T cells was measured by colorimetric reading of 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide reduction, as described previously . Stimulation index (SI) was expressed as percentage of the control.…”

“…Oxidative stress was found to influence the proliferation, activation, cytokine secretion and T‐cell homeostasis . Enhanced intracellular free radical production or reduced antioxidant enzyme activity have been shown to induce T lymphocyte oxidative stress …”

“…Specific cellular immunity can be assayed in vitro by mitogen‐induced lymphocyte proliferation. We have previously used this methodology to test several chemical substances and the results were quite promising . The addition of fatty acids in culture medium of immune cells affects various parameters of the immune response .…”

“…12 Enhanced intracellular free radical production or reduced antioxidant enzyme activity have been shown to induce T lymphocyte oxidative stress. [13][14][15] Modulation of immune system by consumption of vegetable oils protected against some diseases. N-3 polyunsaturated fatty acids (PUFA) improved T cell intracellular oxidative status in diabetes.…”