Effects of Mangaba (Hancornia speciosa) Fruit Extract Adsorbed onto PEG Microspheres in MCF-7 Breast Cancer Cells Co-Cultured with Blood Cells

Abstract

Objective: To evaluate the antitumor effects of polyethylene glycol (PEG) microspheres with adsorbed Hancornia speciosa ethanolic extract (HSEE) on blood mononuclear (MN) cells co-cultured with MCF-7 breast cancer cells. Methods: PEG microspheres were adsorbed with HSEE and examined by flow cytometry and fluorescence microscopy. MCF-7 and MN cells obtained from volunteer donors were pre-incubated alone or co-cultured (MN and MCF-7 cells) for 24 h with or without HSEE, PEG microspheres or PEG adsorbed with HSEE (PEG-HSEE). Cell viability, superoxide release and superoxide dismutase were determined. Results: Fluorescence microscopy showed that PEG microspheres were able to absorb HSEE throughout their surface. Irrespective of the treatment, the viability index of MN cells, MCF-7 and their co-culture was not affected. Superoxide release increased in co-cultured cells treated with HSEE, adsorbed or not onto PEG microspheres. In co-cultured cells, SOD levels in culture supernatant increased in the treatment with HSEE, adsorbed onto PEG microspheres or not. Conclusion: HSEE has direct effects on MN cells co-cultured with MCF-7 cells. The results suggest the benefits of Hancornia speciosa fruit consumption by women at risk of breast cancer. In addition, because PEG-HSEE maintained oxidative balance in co-cultured cells, it is a promising alternative for the treatment of tumor cells.

Pharmaceutical, food potential, and molecular data of Hancornia speciosa Gomes: a systematic review

“…The presence of toxic substances in fruits has not been reported. These are considered functional foods and the results obtained by Araújo ( 2019 ) suggest benefits from the consumption of the Hancornia speciosa fruit by women at risk for breast cancer.…”

Impact of processing and packages on bioactive compounds and antioxidant activity of Mangaba Jelly

“…Indeed, in previous study with male Swiss mice through bone marrow micronucleus test, in vivo gut micronucleus test, comet assay, gut apoptosis cells and oxidative stress was reported a first antimutagenic effect of mangaba fruit pulp (Lima et al, 2015a). Moreover, Araújo et al (2019) recently evaluated the antitumor effect of mangaba extract fruit adsorbed onto polyethylene glycol microsphere in MCF-7 breast cancer cells co-cultured with blood cells and observed that the cell viability was not affected and the superoxide release increased, which suggest that mangaba can be considered as "superfruit" and emphasizes its consumption by women against risk of breast cancer.…”

“…Recently, mangaba fruit has been attracting much attention not only because of its unique flavor and nutritional properties, but also because of its high content of health-promoting phytochemicals, such as vitamin C, vitamin E, carotenoids, folic acid, and phenolic compounds (Lima et al, 2015a, b;Gonçalves et al, 2019;Reis et al, 2019). Various in vitro, animal, and human clinical trials studies have consistently shown that the intake of mangaba fruit is associated with a wide range of pharmacological activities including antioxidant, and antimutagenic (Lima et al, 2015a), anti-cancer (Araújo et al, 2019), and anti-inflammatory (Bitencourt et al, 2019;Reis et al, 2019;Torres-Rêgo et al, 2016). Indeed, in previous study with male Swiss mice through bone marrow micronucleus test, in vivo gut micronucleus test, comet assay, gut apoptosis cells and oxidative stress was reported a first antimutagenic effect of mangaba fruit pulp (Lima et al, 2015a).…”

Bioactive compounds of pequi pulp and oil extracts modulate antioxidant activity and antiproliferative activity in cocultured blood mononuclear cells and breast cancer cells

“…Bioactive compounds of Brazilian Cerrado plants and its antioxidant activity are associated with antiproliferative action in breast cancer cells (3)(4)(5). Pequi (Caryocar brasiliense Camb.)…”