Effects of mannoprotein E1 in liquid diet on inflammatory response and TLR5 expression in the gut of rats infected by Salmonella typhimurium

Abstract

BackgroundMannoproteins are yeast cell wall componend, and rich in mannose. The use of foods rich in mannose as carbohydrate, could have a bioprotective effect against entrobacteria intestinal infection. Nothing is known about mannoproteins' activity in inflammatory bowel processes induced by entrobacteria.This study investigates the effects of mannoprotein administration via a liquid diet on inflammatory response and TLR5 expression during intestinal tissue injury in a rat model of infection with Salmonella typhimurium.MethodsAdult Wistar male rats were divided into three groups: control, and mannoprotein E1 at 10 or 15%. Animals were fed with a liquid diet supplemented or not with mannoprotein E1. Groups were infected by intragastrical administration of S. typhimurium. 24 h post-inoculation samples of spleen, ileum and liver were collected for microbiological studies. Gut samples were processed to determine levels of proinflammatory cytokines (mRNA) and TLR5 (mRNA and protein) by quantitative PCR and Western-blot, and the number of proliferative and apoptotic cells determined by immunohistochemistry.ResultsIninfected levels of proinflammatory cytokines and TLR5 were higher in untreated controls than in the animals receiving mannoprotein. Proliferation was similar in both groups, whereas apoptosis was higher in controls. Curiosly, the mannoprotein effect was dose dependent.ConclusionsMannoprotein administration in a liquid diet seems to protect intestinal tissue against S. typhimurium infection. This protection seems to expressed as a lower pro-inflammatory response and TLR5 downregulation in gut epithelium, as well as by an inhibition of apoptosis. Nevertheless, the molecular mechanism by which mannoprotein is able to regulate these responses remain unclear. These results could open up new avenues in the use of mannoproteins as prebiotics in the therapeutic strategy for treatment of inflammatory gut processes induced by microbia.

Effects of the solubility of yeast cell wall preparations on their potential prebiotic properties in dogs

“…However, a tendency for lower IL-6 and TNF-α in serum was also verified for dogs fed the YCW diet, which did not alter SCFA fecal concentration. These data corroborate findings of other researchers [75], which evaluated the action of a yeast cell wall fraction called "mannoprotein", added to a liquid diet for rats with Salmonella infection. The authors also found lower expression of TNF-α and IL-6 mRNA in the jejunum, ileum and colon tissues in the treatment groups and concluded that yeast cell wall derivates may lower the inflammatory response, protecting the intestinal tissue.…”

Arabinoxylan activates Dectin‐1 and modulates particulate β‐glucan‐induced Dectin‐1 activation

“…To determine if the immune‐modulating effects of arabinoxylan can be explained by binding to PRRs, the activation of TLR2, 4, 5, and Dectin‐1 by arabinoxylan was studied. All these PRRs either recognize carbohydrate groups or are implicated in the recognition of dietary fibers . Reporter cell lines with either human TLR2, TLR4, TLR5, Dectin‐1A, or Dectin‐1B receptors were stimulated with arabinoxylan fibers.…”

Assessment of yeast Saccharomyces cerevisiae component binding to Mycobacterium avium subspecies paratuberculosis using bovine epithelial cells

“…It has also been reported that live yeast stabilizes rumen pH [ 6 , 7 ], and the number of anaerobic cellulolytic bacteria [ 8 , 9 ]. In addition to having nutritional value, there is evidence that yeast probiotics and their components, such as mannan-oligosaccharides (MOS), can adhere to enteric pathogens including Campylobacter jejuni [ 10 ], Escherichia coli serotypes (O2 and O88) [ 11 ], and Salmonella [ 12 , 13 ], thereby reducing their ability to attach to and invade host cells [ 14 ]. Given these properties, it is possible that dietary supplementation with yeast probiotics and/or their components may help protect calves that are vulnerable to Johne’s disease (JD).…”