Effects of Maternal Fenbendazole on Litter Size, Survival Rate, and Weaning Weight in C57BL/6J Mice

Hicks, Kristina L; Roche, Elysia; Wilkerson, James D; Lindstrom, Krista E

doi:10.30802/aalas-jaalas-21-000056

Cited by 4 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The respective diet was fed till the end of delivery after which all maternal mice were fed ad libitum diet. Following birth at day 1 (p1) of age, litter size was culled to three males and three females (average litter size of C57BL/6J mice is 6 25 ), and maternal mice continued to nurse their own pups. The adjustment of litter size was to ensure standardized nursing in each case and to control for differences in maternal care.…”

Section: Maternal Dietmentioning

confidence: 99%

Thermoneutrality effects on developmental programming of obesity

Desai

Torsoni

et al. 2022

J Dev Orig Health Dis

View full text Add to dashboard Cite

Developmental programming studies using mouse models have housed the animals at human thermoneutral temperatures (22°C) which imposes constant cold stress. As this impacts energy homeostasis, we investigated the effects of two housing temperatures (22°C and 30°C) on obesity development in male and female offspring of Control and FR dams. Pregnant mice were housed at 22°C (cold-exposed, CE) or 30°C (thermoneutrality, TN) room temperature. At gestational age e10, mice were fed either an ad libitum diet (Control) or were 30% food-restricted (FR) to produce low birth weight newborns. Following delivery, all dams were fed an ad libitum diet and maternal mice continued to nurse their own pups. At 3 weeks of age, offspring were weaned to an ad libitum diet and housed at similar temperatures as their mothers. Body weights and food intake were monitored. At 6 months of age, body composition and glucose tolerance test were determined, after which, brain and adipose tissue were collected for analysis. FR/CE and FR/TN offspring exhibited hyperphagia and were significantly heavier with increased adiposity as compared to their respective Controls. There was sex-specific effects of temperature in both groups. Male offspring at TN were heavier with increased body fat, though the food intake was decreased as compared to CE males. This was reflected by hypertrophic adipocytes and increased arcuate nucleus satiety/appetite ratio. In contrast, female offspring were not impacted by housing temperature. Thus, unlike female offspring, there was a significant interaction of diet and temperature evident in the male offspring with accentuated adverse effects evident in FR/TN males.

show abstract

Section: Maternal Dietmentioning

confidence: 99%

Thermoneutrality effects on developmental programming of obesity

Desai

Torsoni

et al. 2022

J Dev Orig Health Dis

View full text Add to dashboard Cite

show abstract

“…Fenbendazole (FBZ) is a broad spectrum benzimidazole anthelmintic that is commonly used in laboratory animals for the treatment of pinworms [1]. The drug is regarded as safe, including use during pregnancy, and has minimal side effects [1]. FBZ impregnated feed is the current treatment of choice to eliminate rodent internal parasites.…”

Section: Introductionmentioning

confidence: 99%

Effects of fenbendazole on fecal microbiome in BPH/5 mice, a model of hypertension and obesity, a brief report

Beckers¹,

Schulz²,

Liu³

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

Fenbendazole (FBZ) is a common antiparasitic treatment used in research rodent colonies for biosecurity purposes. The effect of this compound has been studied in C57 mice, but never before in a strain of mice that has co-morbidities, such as the blood pressure high (BPH)/5. The BPH/5 mouse is an inbred genetic model of hypertension. While both male and female BPH/5 have high blood pressure, there is a metabolic sexual dimorphism with females displaying key features of obesity. The obese gut microbiome has been linked to hypertension. Therefore, we hypothesized that fenbendazole treatment will alter the gut microbiome in hypertensive mice in a sex dependent manner. To test the influence of FBZ on the BPH/5 gut microbiota, fecal samples were collected pre- and post-treatment from adult BPH/5 mice (males and non-pregnant females). The mice were treated with fenbendazole impregnated feed for five weeks. Post-treatment feces were collected at the end of the treatment period and DNA was extracted, and the V4 region of 16S rRNA was amplified and sequenced using the Illumina MiSeq system. The purpose was to analyze the fecal microbiome before and after FBZ treatment, the results demonstrate changes with treatment in a sex dependent manner. More specifically, differences in community composition were detected in BPH/5 non-pregnant female and males using Bray-Curtis dissimilarity as a measure of beta-diversity (treatment p = 0.002). The ratio of Firmicutes to Bacteroidetes, which has been identified in cases of obesity, was not altered. Yet, Verrucomicrobia was increased in BPH/5 males and females post-treatment and was significantly different by sex (treatment p = 5.85e-05, sex p = 0.0151, and interaction p = 0.045), while Actinobacteria was decreased in the post-treatment mice (treatment p = 0.00017, sex p = 0.5, interaction p = 0.2). These results are indicative of gut dysbiosis compared to pre-treatment controls. Lactobacillus was decreased with FBZ treatment in BPH/5 females only. In conclusion, fenbendazole does alter the gut microbial communities, most notable in the male rather than female BPH/5 mouse. This provides evidence that caution should be taken when providing any gut altering treatments before or during mouse experiments.

show abstract