Effects of mechanical vibration on proliferation and osteogenic differentiation of human periodontal ligament stem cells

Zhang, Chunxiang; Ji, Li; Zhang, Linkun; Zhou, Yi; Hou, Weiwei; Quan, Huixin; Li, Xiaoyu; Chen, Yangxi; Yu, Haiyang

doi:10.1016/j.archoralbio.2012.04.010

Cited by 97 publications

(91 citation statements)

References 49 publications

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“…Osterix is a recently discovered transcription factor related to bone formation as well as osteogenic differentiation. It contains a zinc finger motif that is specifically expressed in osteoblasts [7,8] Studies have shown that mechanical stimulation can induce high levels of osterix mRNA expression [9][10][11]. However, current studies have not produced information regarding the relationship between the p38MAPK signaling pathway and osterix during osteogenic differentiation of BMMSCs induced by intermittent stretching.…”

Section: Introductionmentioning

confidence: 67%

Intermittent Stretching and Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells via the p38MAPK-Osterix Signaling Pathway

Xiao

Zhang

Fan

et al. 2015

Cell Physiol Biochem

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Aims: The relationship between the p38MAPK signaling pathway and osterix in osteogenic differentiation of BMMSCs subjected to intermittent stretching was investigated. Methods: BMMSCs derived from C57BL/6J mice were divided into the following groups: 1) control, 2) stretch, and 3) SB203580+stretch (SB203580 is a p38MAPK signal pathway inhibitor). BMMSCs were exposed to an intermittent mechanical strain of 0.8% (8000μ strain) at 0.5 Hz, twice a day for 30 min each application. BMMSCs were harvested on days 1, 3, and 5 post-treatment. The expression of ALP, COL I, OCN, and osterix mRNA was assessed utilizing RT-PCR while the expression of P-p38MAPK and osterix protein was assessed by Western blot analysis. The osterix gene in mouse BMMSCs was knocked down using RNAi technology and its protein expression was also assessed by Western blot. RT-PCR was used to detect ALP, COL I, and OCN mRNA expression. Results: Intermittent stretching was found to promote expression of ALP, COL I, OCN, and osterix mRNA. Silencing the osterix gene was found to reduce levels of ALP, COL I, and OCN mRNA. Western blot analysis demonstrated that the levels of osterix and P-p38MAPK proteins in the stretch group were significantly higher than in the control group (P<0.05). There was less expression of ALP, COL I, OCN, and osterix mRNA in the SB203580+stretch group than in the control and stretch groups. Conclusions: Data demonstrate that intermittent stretching promotes osteogenic differentiation of BMMSCs, and the p38MAPK-osterix pathway has an important role in the control of osteogenesis-related gene expression.

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Section: Introductionmentioning

confidence: 67%

Intermittent Stretching and Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells via the p38MAPK-Osterix Signaling Pathway

Xiao

Zhang

Fan

et al. 2015

Cell Physiol Biochem

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“…In addition to the growth factor stimulation, mechanical stimulation can also affect osteogenic differentiation of stem cells [8,9]. Intracellular signalling pathways induced by mechanical stimulation in neurosensory and vascular cells can induce physiological responses which are much more rapid [10] than those induced by growth factors which can require minutes to hours [11].…”

Section: Introductionmentioning

confidence: 99%

Low-Frequency Mechanical Stimulation Modulates Osteogenic Differentiation of C2C12 Cells

et al. 2013

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Mechanical stimulation influences stem cell differentiation and may therefore provide improved lineage specification control for clinical applications. Low-frequency oscillatory mechanical stimulation (0.01 Hz) has recently been shown to suppress adipogenic differentiation of mesenchymal stem cells, indicating that the range of effective stimulation frequencies is not limited to those associated with locomotion, circulation, and respiration. We hypothesized that low-frequency mechanical stimulation (0.01 Hz) can also promote osteogenic cell differentiation of myoblastic C2C12 cells in combination with BMP-2. Results indicate that lowfrequency mechanical stimulation can significantly enhance osteogenic gene expression, provided that differentiation is initiated by a priming period involving BMP-2 alone. Subsequent application of low-frequency mechanical stimulation appears to act synergistically with continued BMP-2 exposure to promote osteogenic differentiation of C2C12 cells and can even partially compensate for the removal of BMP-2. These effects may be mediated by the ERK and Wnt signalling pathways. Osteogenic induction of C2C12 cells by low-frequency mechanical stimulation is therefore critically dependent upon previous exposure to growth factors, and the timing of superimposed BMP-2 and mechanical stimuli can sensitively influence osteogenesis. These insights may provide a technically simple means for control of stem cell differentiation in cell-based therapies, particularly for the enhancement of differentiation toward desired lineages.

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“…In vitro, weak intermittent vibratory forces can induce expression of RANKL in human periodontal ligament stem cells under optimal compression forces. 2 A study 3 in humans showed vibratory stimuli provided no clinical advantage for early resolution of crowding or alleviation of pain during initial alignment. In contrast, a number of studies 4,5 have reported that short durations of low-magnitude, high-frequency resonance vibration combined with orthodontic force can increase the rate of orthodontic tooth movement without additional tissue damage in humans.…”

Section: Introductionmentioning

confidence: 99%

Vibratory stimulation increases interleukin-1 beta secretion during orthodontic tooth movement

Leethanakul

Suamphan

Jitpukdeebodintra

et al. 2015

The Angle Orthodontist

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Objectives: To investigate the effects of application of vibratory stimuli on interleukin (IL)-1b secretion during maxillary canine distalization. Materials and Methods: Split-mouth design study in 15 subjects (mean age, 22.9 years; range 19-25 years) whose bilateral maxillary first premolars were extracted with subsequent canine distalization. On the experimental side, light force (60 g) was applied to the canine for 3 months in combination with vibratory stimuli provided using an electric toothbrush 15 minutes a day for 2 months; only orthodontic force was applied to the contralateral control canine. Gingival crevicular fluid (GCF) was collected from the mesial and distal sides of each canine at each monthly appointment. IL-1b levels were analyzed using an enzyme-linked immunosorbent assay. Canine movement was measured monthly. Results: Overall, enhanced IL-1b secretion was observed at the pressure sites of experimental canines compared to control canines (mean, 0.64 6 0.33 pg/mL vs 0.10 6 0.11 pg/mL, respectively, P , .001). The accumulative amount of tooth movement was greater for the experimental canine than for the control canine (mean, 2.85 6 0.17 mm vs 1.77 6 0.11 mm, respectively, P , .001).Conclusions: This study demonstrates that, in combination with light orthodontic force, application of vibratory stimuli using an electric toothbrush enhanced the secretion of IL-1b in GCF and accelerated orthodontic tooth movement. (Angle Orthod. 2016;86:74-80.)

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Effects of mechanical vibration on proliferation and osteogenic differentiation of human periodontal ligament stem cells

Cited by 97 publications

References 49 publications

Intermittent Stretching and Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells via the p38MAPK-Osterix Signaling Pathway

Intermittent Stretching and Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells via the p38MAPK-Osterix Signaling Pathway

Low-Frequency Mechanical Stimulation Modulates Osteogenic Differentiation of C2C12 Cells

Vibratory stimulation increases interleukin-1 beta secretion during orthodontic tooth movement

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