Vibratory stimulation increases interleukin-1 beta secretion during orthodontic tooth movement

Abstract

Objectives: To investigate the effects of application of vibratory stimuli on interleukin (IL)-1b secretion during maxillary canine distalization. Materials and Methods: Split-mouth design study in 15 subjects (mean age, 22.9 years; range 19-25 years) whose bilateral maxillary first premolars were extracted with subsequent canine distalization. On the experimental side, light force (60 g) was applied to the canine for 3 months in combination with vibratory stimuli provided using an electric toothbrush 15 minutes a day for 2 months; only orthodontic force was applied to the contralateral control canine. Gingival crevicular fluid (GCF) was collected from the mesial and distal sides of each canine at each monthly appointment. IL-1b levels were analyzed using an enzyme-linked immunosorbent assay. Canine movement was measured monthly. Results: Overall, enhanced IL-1b secretion was observed at the pressure sites of experimental canines compared to control canines (mean, 0.64 6 0.33 pg/mL vs 0.10 6 0.11 pg/mL, respectively, P , .001). The accumulative amount of tooth movement was greater for the experimental canine than for the control canine (mean, 2.85 6 0.17 mm vs 1.77 6 0.11 mm, respectively, P , .001).Conclusions: This study demonstrates that, in combination with light orthodontic force, application of vibratory stimuli using an electric toothbrush enhanced the secretion of IL-1b in GCF and accelerated orthodontic tooth movement. (Angle Orthod. 2016;86:74-80.)

Aligning Effectiveness, Secretion of Interleukin 1β and Pain Control During Fixed Orthodontic Treatment with Self-Ligating Appliances and Supplemental Vibrational Appliances. A Randomized Controlled Clinical Trial

“…In agreement with these findings Leethanakul et al found higher concentration of IL-1β in canines after electric toothbrush application for 2 months (16). Interestingly, in their study a higher rate of maxillary canine distalization was also reported.…”

“…Contrary to these findings, Pavlin et al (19) reported that low-level cycling loading of 0.25N at 30 Hz released by the same vibrational appliance used in the study of Miles et al increases the rate of orthodontic tooth movement (OTM) when applied as adjunct to orthodontic treatment. In a recent study Leethankul et al investigated through a split-mouth study the levels of IL-1β in gingival cervicular fluid and the rate of orthodontic tooth movement in canines stimulated by electric toothbrush compared to standard orthodontic movement of canines (16). They found enhanced secretion of IL-1β and accelerated OTM in canines stimulated by electric toothbrush vibrations.…”

“…On the other hand, an increase of bone mass could be induced by vibration of a certain frequency, intensity and duration (13). Based on promising results of few animal studies on potential acceleratory effects of vibrations, (12,14) several human studies were undertaken in order to evaluate the effect of vibrational forces on orthodontic tooth movement (11,(15)(16)(17)(18). However, contrasting results emerged by different studies.…”

Biochemical markers of bone metabolism during early orthodontic tooth movement with aligners

“…21 The GCF absorbed on each paper point was diluted with 250 lL of sterile phosphate-buffered saline (pH 7.4), centrifuged (13,000 g at 48C for 15 minutes), and stored at À808C until analysis. 22 The Periotron was always calibrated before GCF sampling. 21 …”

Accelerated Orthodontics–An overview

“…The vibration tests were carried out for 5 minutes, and the resonance curves were displayed as frequency-force relationships on the monitor of the vibration controller. Clinical trials were conducted by various researchers [43][44][45][46][47] on human population using oral vibrating devices such as AccledentTM, AcceleDent® and electric tooth brushes and found to be effective in increasing the rate of tooth movement. Two cross sectional animal studies [43,44] done on cyclic vibration.…”