Effects of mesenchymal stromal cells on type 1 diabetes mellitus rat muscles

Sabadine, Maria Augusta; Russo, Thiago Luiz; Luna, Genoveva Lourdes Flores; Leal, Ângela M. O.

doi:10.1002/mus.26196

Cited by 5 publications

(6 citation statements)

References 50 publications

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“…In diabetic rats, motor activity, CSA and the number of fibres were reduced. The reduction of myofibres and muscle mass was associated with both increased protein degradation and decreased protein synthesis in skeletal muscles causing the muscle weakness in T1D. The more pronounced atrophy of white muscles is observed in muscle wasting disorders, and the responses of red muscles to STZ and/or MSC therapy may differ .…”

Section: Discussionmentioning

confidence: 99%

“…However, the observed strong positive correlation between CSA and motor activity is noteworthy. It has been previously reported that the walking performance and motor dysfunction in diabetic rats are associated with skeletal muscle fibrosis and decreased motor units and that the local and systemic transplantation of MSCs contribute to myogenesis, cellular differentiation in muscle inflammatory and degenerative condition models improving the repair process. However, the molecular mechanism of action of MSCs on muscle fibre repair and motor function improvement is not fully understood …”

Section: Discussionmentioning

confidence: 99%

“…There is little evidence regarding the effects of MSCs on muscular dysfunction caused by DM. It was previously demonstrated that MSC transplantation improved walking performance and decreased muscle fibrosis in DM by modulating atrogenes . However, the relationship between motor function and muscle collagen organization was not investigated.…”

Section: Introductionmentioning

confidence: 99%

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Effects of mesenchymal stromal cells on motor function and collagen in the skeletal muscles of rats with type I diabetes

Luna

Russo

Sabadine

et al. 2019

Int J Experimental Path

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Summary The present study aimed to evaluate the effects of mesenchymal stromal cell (MSC) transplantation on motor function and collagen organization in the muscles of rats with type 1 diabetes mellitus. Male Wistar rats were randomly assigned to three groups: control (C), diabetic (DM) and diabetic treated with MSCs (DM‐MSCs). Diabetes was induced by streptozotocin (50 µg/kg). Bone marrow cells were isolated from the tibia and femur. After 10 weeks of DM induction, the DM‐MSC rats received four i.p. injections of MSCs (1 × 106). Ten weeks after MSC transplantation, motor performance was evaluated by the rotarod test and the anterior tibial (TA) muscles were collected for morphometric and quantification of collagen birefringence by polarizing microscopy analysis. Motor performance of the DM group was significantly reduced when compared to the C group and increased significantly in the DM + MSC group. The TA muscle mass was significantly reduced in the DM and DM + MSC groups compared to the C group. The connective tissue increased in the DM group compared to the C group and decreased in the DM + MSC group. The percentage collagen birefringence decreased significantly in the DM group when compared to the C group and increased in the DM + MSC group. Motor performance was positively correlated with collagen birefringence and negatively correlated with percentage of connective tissue. The results indicate that MSC transplantation improves both motor function and the collagen macromolecular organization in type 1 DM.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of mesenchymal stromal cells on motor function and collagen in the skeletal muscles of rats with type I diabetes

Luna

Russo

Sabadine

et al. 2019

Int J Experimental Path

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“…Treatment consists mainly of protective lung ventilation strategies and early fluid resuscitation. Despite the continuous improvement in treatment methods, the mortality rate remains as high as 40%-60% [ 2 ]. The lack of specific etiological treatment measures is an important factor in the high mortality rate of ARDS.…”

Section: Introductionmentioning

confidence: 99%

“…MSCs can also secrete a variety of cytokines to exert anti-inflammatory and repair effects. In recent years, a large number of studies have shown that MSCs can effectively treat a variety of diseases, including myocardial injury and diabetes [ 1 , 2 ]. In ARDS, MSCs exert a significant therapeutic effect, both in animals and humans, showing its unique and excellent anti-inflammatory and antidamage properties [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Vimentin-Rab7a Pathway Mediates the Migration of MSCs and Lead to Therapeutic Effects on ARDS

Wang

Zhang

et al. 2021

Stem Cells International

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Acute respiratory distress syndrome (ARDS) is difficult to treat and has a high mortality rate. Mesenchymal stem cells (MSCs) have an important therapeutic effect in ARDS. While the mechanism of MSC migration to the lungs remains unclear, the role of MSCs is of great clinical significance. To this end, we constructed vimentin knockout mice, extracted bone MSCs from the mice, and used them for the treatment of LPS-induced ARDS. H&E staining and Masson staining of mouse lung tissue allowed us to assess the degree of damage and fibrosis of mouse lung tissue. By measuring serum TNF-α, TGF-β, and INF-γ, we were able to monitor the release of inflammatory factors. Finally, through immunoprecipitation and gene knockout experiments, we identified upstream molecules that regulate vimentin and elucidated the mechanism that mediates MSC migration. As a result, we found that MSCs from wild-type mice can significantly alleviate ARDS and reduce lung inflammation, while vimentin gene knockout reduced the therapeutic effect of MSCs in ARDS. Cytological experiments showed that vimentin gene knockout can significantly inhibit the migration of MSCs and showed that it changes the proliferation and differentiation status of MSCs. Further experiments found that vimentin’s regulation of MSC migration is mainly mediated by Rab7a. Rab7a knockout blocked the migration of MSCs and weakened the therapeutic effect of MSCs in ARDS. In conclusion, we have shown that the Vimentin-Rab7a pathway mediates migration of MSCs and leads to therapeutic effects in ARDS.

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The safety and efficacy of stem cell therapy for diabetic peripheral neuropathy in animal studies: A systematic review and meta-analysis

Alizadeh,

Hassan Zadeh Tabatabaei,

Rezaei Zadeh Rukerd

et al. 2025

Neuroscience

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Effects of mesenchymal stromal cells on type 1 diabetes mellitus rat muscles

Abstract: MSCs can reduce muscle and functional complications that result from type 1 DM in rats. Muscle Nerve 58: 583-591, 2018.

Cited by 5 publications

References 50 publications

Effects of mesenchymal stromal cells on motor function and collagen in the skeletal muscles of rats with type I diabetes

Effects of mesenchymal stromal cells on motor function and collagen in the skeletal muscles of rats with type I diabetes

Vimentin-Rab7a Pathway Mediates the Migration of MSCs and Lead to Therapeutic Effects on ARDS

The safety and efficacy of stem cell therapy for diabetic peripheral neuropathy in animal studies: A systematic review and meta-analysis

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