Effects of meta–topolin derivatives and temporary immersion on hyperhydricity and in vitro shoot proliferation in Pyrus communis

Lotfi, Mariem; Bayoudh, Chokri; Werbrouck, Stefaan; Mars, Messaoud

doi:10.1007/s11240-020-01935-x

Cited by 15 publications

(9 citation statements)

References 41 publications

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“…The multiplication of both clones was more efficient in bioreactors than in jars with semisolid medium. Similar results have been reported for other woody plants such as calabash tree [39], eucalyptus [40], apple [41], teak [42,43], pistachio [44], chestnut [45], hazelnut [46], yerba mate [47], willow [48], olive [49], alder [50] and pear [51].…”

Section: Discussionsupporting

confidence: 87%

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

et al. 2022

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In this study, we propagated two old Galician plum varieties in liquid medium using a temporary immersion system with RITA© bioreactors. Environmental variables including culture system, light intensity, CO2 enrichment, immersion frequency and sucrose supplementation were evaluated in relation to in vitro proliferation, physiological status and ex vitro performance. Bioreactors were superior to jars for culturing shoots in photomixotrophic conditions, producing up to 2 times more shoot numbers and up to 1.7 times more shoot length (depending on the genotype) using shoot clusters. The number and quality of shoots were positively influenced by the sucrose concentration in the medium, plus by the light and gaseous environment. For individual apical sections the best response occurred with 3% sucrose, 150 µmol m−2 s−1 photosynthetic photon flux density and 2000 ppm CO2, averaging 2.5 shoots per explant, 26 mm shoot length and 240 mm2 leaf area, while with 50 µmol m−2 s−1 light and ambient CO2 (400 ppm) values decreased to 1.2 shoots per explant, 14 mm of shoot length and 160 mm2 of leaf area. Shoots cultured photoautotrophically (without sucrose) were successfully rooted and acclimated despite of showing limited growth, low photosynthetic pigments, carbohydrate, phenolic and antioxidant contents during the multiplication phase.

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Section: Discussionsupporting

confidence: 87%

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

et al. 2022

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“…For Lippia grata Schauer shoots, the combination of partial sealing with red/blue LED at a 1:1 light ratio resulted in a 61% reduction of hyperhydric leaves in comparison with traditional conditions [ 89 ]. The quality of Pyrus communis L. shoots cultivating under far-red LED was inferior because of hyperhydricity and chlorosis, as indicated by their low chlorophyll and carotenoid content [ 90 ].…”

Section: Influence Of Medium Composition and Culture Conditions On Hy...mentioning

confidence: 99%

Hyperhydricity in Plant Tissue Culture

Polivanova

Bedarev

2022

Plants

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Hyperhydricity is the most common physiological disorder in in vitro plant cultivation. It is characterized by certain anatomical, morphological, physiological, and metabolic disturbances. Hyperhydricity significantly complicates the use of cell and tissue culture in research, reduces the efficiency of clonal micropropagation and the quality of seedlings, prevents the adaptation of plants in vivo, and can lead to significant losses of plant material. This review considers the main symptoms and causes of hyperhydricity, such as oxidative stress, impaired nitrogen metabolism, and the imbalance of endogenous hormones. The main factors influencing the level of hyperhydricity of plants in vitro are the mineral and hormonal composition of a medium and cultivation conditions, in particular the aeration of cultivation vessels. Based on these factors, various approaches are proposed to eliminate hyperhydricity, such as varying the mineral and hormonal composition of the medium, the use of exogenous additives, aeration systems, and specific lighting. However, not all methods used are universal in eliminating the symptoms of hyperhydricity. Therefore, the study of hyperhydricity requires a comprehensive approach, and measures aimed at its elimination should be complex and species-specific.

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“…Whether this alteration resulted in a positive or negative effect remains being a subject of controversy. Several studies have reported the negative impact of endophytic contaminations on in vitro cultures during the last years (Dunaeva & Osledkin, 2015;Lotfi et al, 2020;Thomas, 2004Thomas, , 2011. In addition, some endophytic contaminants lead to the loss of valuable research material since they can overrun the plant cultures (Cassells, 2012).…”

Section: In Vitro Biological Control Activity By Pgpmsmentioning

confidence: 99%

Using plant growth-promoting microorganisms (PGPMs) to improve plant development under in vitro culture conditions

Cantabella¹,

Dolcet-Sanjuan²,

Teixidó³

2022

Planta

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Plant in vitro culture techniques are highly useful to obtain significant amounts of true-to-type and disease-free plant materials. One of these techniques is clonal micropropagation which consists on the establishment of shoot tip cultures, shoot multiplication, in vitro rooting and acclimatization to ex vitro conditions. However, in some cases, the existence of recalcitrant genotypes, with a compromised multiplication and rooting ability, or the difficulties to overcome the overgrowth of endophytic contaminations might seriously limit its efficiency. In this sense, the establishment of beneficial interactions between plants and plant growth-promoting microorganisms (PGPMs) under in vitro culture conditions might represent a valuable approach to efficiently solve those restrictions. During the last years, significant evidence reporting the use of beneficial microorganisms to improve the yield of in vitro multiplication or rooting as well as their acclimatization to greenhouse or soil conditions have been provided. Most of these positive effects are strongly linked to the ability of these microorganisms to provide in vitro plants with nutrients such as nitrogen or phosphorous, to produce plant growth regulators, to control the growth of pathogens or to mitigate stress conditions. The culture of A. thaliana under aseptic conditions has provided high-quality knowledge on the root development signalling pathways, involving hormones, triggered in the presence of PGPMs. Overall, the present article offers a brief overview of the use of microorganisms to improve in vitro plant performance during the in vitro micropropagation stages, as well as the main mechanisms of plant growth promotion associated to these microorganisms. Main conclusion:The use of beneficial microorganisms improves the performance of in vitro cultured plants through the improvement of plant nutrition, the biological control of microbial pathogens or the production of phytohormones that promote plant growth and development.

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Effects of meta–topolin derivatives and temporary immersion on hyperhydricity and in vitro shoot proliferation in Pyrus communis

Cited by 15 publications

References 41 publications

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

Micropropagation of Plum (Prunus domestica L.) in Bioreactors Using Photomixotrophic and Photoautotrophic Conditions

Hyperhydricity in Plant Tissue Culture

Using plant growth-promoting microorganisms (PGPMs) to improve plant development under in vitro culture conditions

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