Effects of methanol extract of chansu on hypothalamic-pituitary-testis function in rats

Wang, Shyi-Wu; Lin, Hong-Da; Tsai, Shiow-Chwen; Hwu, Chii‐Min; Chiao, Yu-Chung; Lu, Chunliang; Chen, Jiann‐Jong; Wang, Guei-Jane; Chou, Cheng‐Jen; Lin, Lie‐Chwen; Chen, Chieh-Fu; Wong, Paulus S.

doi:10.1016/s0026-0495(98)90325-0

Cited by 11 publications

(6 citation statements)

References 20 publications

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“…Chan Su has been applied in numerous clinical situations; however, the bioactive compounds in Chan Su are not fully known. Until recently, only a few studies have reported on its pharmacological effects, and bufadienolides, including bufalin, are generally agreed upon to be the major bioactive components (19,23). Several studies have demonstrated the potency of Chan Su and its major components in treatment of various cultured human cancer cells by induction of cell cycle arrest and apoptosis (23–28).…”

Section: Introductionmentioning

confidence: 99%

“…Until recently, only a few studies have reported on its pharmacological effects, and bufadienolides, including bufalin, are generally agreed upon to be the major bioactive components (19,23). Several studies have demonstrated the potency of Chan Su and its major components in treatment of various cultured human cancer cells by induction of cell cycle arrest and apoptosis (23–28). The significance of Chan Su in induction of apoptosis against human bladder carcinoma cells, which was mediated by modulation of Bcl-2 family proteins and proteolytic activation of caspases, has recently been reported (29).…”

Section: Introductionmentioning

confidence: 99%

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Apoptotic and anti-metastatic effects of the whole skin of Venenum bufonis in A549 human lung cancer cells

Park¹,

Shin²,

Lee³

et al. 2011

International Journal of Oncology

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In the present study, the effects of the whole skin of Venenum bufonis on apoptotic and anti-invasive activity in A549 human lung cancer cells were investigated. Treatment with extract of the whole skin of V. bufonis (SVB) resulted in a significant decrease in cell growth of A549 cells, depending on dosage, which was associated with apoptosis induction, as proved by chromatin condensation and accumulation of apoptotic fraction. SVB treatment induced expression of death receptor-related proteins, such as death receptor 4, which further triggered activation of caspase-8 and cleavage of Bid. In addition, the increase in apoptosis by SVB treatment was correlated with dysfunction of mitochondria, activation of caspase-9 and -3, downregulation of IAP family proteins, such as XIAP, cIAP-1 and cIAP-2, and concomitant degradation of activated caspase-3-specific target proteins, such as poly (ADP-ribose) polymerase and β-catenin proteins. However, z-DEVD-fmk, a caspase-3-specific inhibitor, blocked SVB-induced apoptosis and increased the survival rate of SVB-treated cells, indicating that activation of caspase-3 plays a key role in SVB-induced apoptosis. In addition, within concentrations that were not cytotoxic to A549 cells, SVB induced marked inhibition of cell motility and invasiveness. Activities of matrix metalloproteinase (MMP)-2 and MMP-9 in AGS cells were dose-dependently inhibited by treatment with SVB, and this was also correlated with a decrease in expression of their mRNA and proteins, and upregulation of tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 mRNA expression. Further studies are needed; however, the results indicated that SVB induces apoptosis of A549 cells through a signaling cascade of death receptor-mediated extrinsic as well as mitochondria-mediated intrinsic caspase pathways. Our data also demonstrated that MMPs are critical targets of SVB-induced anti-invasiveness in A549 cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Apoptotic and anti-metastatic effects of the whole skin of Venenum bufonis in A549 human lung cancer cells

Park¹,

Shin²,

Lee³

et al. 2011

International Journal of Oncology

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“…They have been reported to show significant activities in inducing the apoptosis against endometriosis and bladder carcinoma [6,7], inhibiting the proliferation of prostate cancer cell lines [8], the colchicine-resistant primary liver carcinoma cell line PLC/PRF/5 [9] and exhibiting antimicrobial activity against Staphylococcus aureus and Escherichia coli [10]. Meanwhile, as cardioactive steroids, bufadienolides are also observed to possess the adverse effects such as contribution to cardiomyopathy [11], impairment of renal tubular sodium-calcium exchange efficiency [12], and inhibition of testosterone secretion in male rat [13], etc. Therefore, the detection and quantification of bufadienolides concentrations in plasma are very important for drug monitoring on preclinical studies and further investigations.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination and pharmacokinetics of five bufadienolides in rat plasma after oral administration of Chansu extract by SPE-HPLC method

Liang

Liu

Qin

et al. 2008

Journal of Pharmaceutical and Biomedical Analysis

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“…It was found that the administration of ginsenoside-Rb, Chansu extracts or bufalin to male rats diminished the LH response to GnRH (Tsai et al, 2003;Wang et al, 1998;Wang et al, 1997). It was not known whether LH secretion by AP was altered by AHM.…”

Section: Discussionmentioning

confidence: 99%

Effect of adlay (Coix lachryma‐jobi L. var. ma‐yuen Stapf.) hull extracts on testosterone release from rat Leydig cells

Hsia

Tseng

Wang

et al. 2008

Phytotherapy Research

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Adlay has been used as a traditional Chinese medicine for the treatment of many diseases. However, few studies have reported the effects of adlay seeds on the endocrine system. In the present study, the effects of methanol extracts of adlay hull (AHM) on testosterone synthesis were studied. Rat Leydig cells were incubated with different reagents including human chorionic gonadotropin, 8-bromo-adenosine-3',5'-cyclic monophosphate, forskolin, A23187, progesterone and androstenedione in the presence or absence of AHM. The rat anterior pituitary (AP) gland was treated with gonadotropin-releasing hormone (GnRH) in vitro in the presence or absence of AHM, and the concentrations of luteinizing hormone (LH) in the media were measured. AHM decreased testosterone release via the inhibition of (1) the PKA and PKC signal transduction pathways, (2) 17beta-HSD enzyme activity in rat Leydig cells, and (3) in vitro GnRH-induced LH secretion.

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Effects of methanol extract of chansu on hypothalamic-pituitary-testis function in rats

Cited by 11 publications

References 20 publications

Apoptotic and anti-metastatic effects of the whole skin of Venenum bufonis in A549 human lung cancer cells

Apoptotic and anti-metastatic effects of the whole skin of Venenum bufonis in A549 human lung cancer cells

Simultaneous determination and pharmacokinetics of five bufadienolides in rat plasma after oral administration of Chansu extract by SPE-HPLC method

Effect of adlay (Coix lachryma‐jobi L. var. ma‐yuen Stapf.) hull extracts on testosterone release from rat Leydig cells

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