Effects of Methyl Jasmonate and Putrescine on Tryptanthrin and Indirubin Production in in vitro Cultures of Isatis demiriziana Mısırdalı

Abstract

Tryptantrin and indirubin are pharmacologically active compounds used in treatment of disseases such as cancer and Alzheimer's. In this study, we investigated the influences of different concentrations of methyl jasmonate (MeJa) and putrescine (Put) on tryptanthrin and indirubin production in leaf explants and development of Isatis demiriziana Mısırdalı grown in vitro. In all media treated with methyl jasmonate, tryptanthrin production in leaves of plantlets showed an increase. The highest increase in tryptanthrin production was observed in solid Murashige-Skoog (MS) medium containing 1.0 mM MeJa (154.026 ± 0.11 µg g-1), about 2.85-fold higher than the control (untreated plantlets) (40.017 ± 0.031 µg g-1). Production of tryptanthrin decreased about 2.56-fold in the leaves of plantlets treated with Put, when compared to control. The highest indirubin production was obtained in the leaves of plantlets grown in the MS medium containing 0.1 mM MeJa (11.274 ± 0.035 µg g-1) but treatments with Put didn't show any positive affect on the indirubin production. Analysis of tryptanthrin and indirubin were performed using high performance liquid chromatography (HPLC).

Anti‐microtubule activity of the traditional Chinese medicine herb Northern Ban Lan (Isatis tinctoria) leads to glucobrassicin

“…To resolve the biological function of a compound it is useful to consider the conditions under which it accumulates. In fact, glucobrassicins in Isatis accumulate in response to jasmonic acid, but also wounding (Galletti et al, 2006; Karakaş, 2019), clearly supporting a role in warding off herbivores. Actually, the name goitrin derives from the ability of these compounds to induce human goiters through the milk from cows that feed on Brassicaceae (Michajlovskij et al, 1970).…”

Effect of Silver Nanoparticles on Production of Indole Alkaloids in Isatis constricta

“…Particularly, I. tinctoria and I. indigotica have been used in modern and traditional medicine for the treatment of hepatitis, viral pneumonia, influenza, snake bites, hemorrhoids and various inflammatory diseases (Chang et al 2012;Hamburger 2002). Diverse bioactive constituents have been reported from methanol extracts of leaves and roots of the Isatis species (Liau et al 2007;Karakas 2019). Alkaloids, which are used in both treatments and source of dyestuff, are evaluated as characteristic compounds of these plants (Bektas et al 2016).…”