Effects of methylmercuric chloride in the presence of cyclic AMP-stimulating agents on glioma and neuroblastoma cells in culture

Prasad, Kedar N.; Nobles, Eileen; Spuhler, Karen

doi:10.1016/0013-9351(79)90059-8

Cited by 16 publications

(2 citation statements)

References 16 publications

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“…In the preceding article, we discussed literature reports describing MeHg-induced elevations of both cyclic AMP and Ca2+ in neuroblastoma and synapto-soma1 systems, respectively (Prasad et al, 1979;Komulainen and Bondy, 1987). Activation of second messenger systems such as these would produce increased phosphorylation of a large subset of protein substrates for Ca2+/calmodulin-dependent protein kinase, protein kinase C, and/or cyclic AMP-dependent protein kinase.…”

Section: Discussionmentioning

confidence: 99%

Altered Patterns of Protein Phosphorylation and Synthesis Caused by Methyl Mercury in Cerebellar Granule Cell Culture

Sarafian

Verity

1990

Journal of Neurochemistry

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In the preceding report we demonstrated a dose-dependent increase in 32P-phosphoprotein labeling after 24-h exposure of cultured cerebellar granule neurons to methyl mercury (MeHg), a response that was not observed in glial cultures. In the present study we have examined 32P-labeled phosphoproteins by two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. At concentrations of 0.5 and 1 microM, which were not extensively cytotoxic, MeHg enhanced phosphorylation of numerous acidic proteins, particularly a cluster of proteins with Mr approximately 28,000 and pI approximately 5.7-5.9 (pp 28/5.7-5.9) and a protein with Mr approximately 58,000 and pI approximately 5.6. The pp28 cluster displayed considerable two-dimensional pattern variability from one experiment to the next, suggesting susceptibility to subtle structural modifications. Time course studies revealed that increased 32P phospholabeling of pp28/5.7-5.9 was detectable after 12-h exposure to 3 microM MeHg and reached values of 300-500% of control by 24 h. These studies also showed that among the 21 proteins analyzed by two-dimensional densitometry, 32P phospholabeling of four proteins increased by 20-50% and of two proteins decreased by 20-50% after 24-h treatment. However, exposure to 10 microM MeHg produced stimulation of pp28/5.7-5.9 32P phospholabeling within 2 h. Under these conditions a relatively high stimulation (sevenfold) of pp28/5.7 phospholabeling occurred, while pp28/5.9 32P phospholabeling was only moderately (5-20%) enhanced. 35S and 32P double-label analysis of cells treated with 0, 0.5, and 1 microM MeHg indicated specific stimulation of 32P phospholabeling of these proteins without increased polypeptide synthesis.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Altered Patterns of Protein Phosphorylation and Synthesis Caused by Methyl Mercury in Cerebellar Granule Cell Culture

Sarafian

Verity

1990

Journal of Neurochemistry

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“…In vitro concentrations of 0.3 µM-1.0 µM CH 3 HgCI produce disturbances in high affinity uptake of certain putative neurotransmitters. Previous shortterm studies have shown [9][10][11][12] that these concentrations of CH 3 HgCl inhibit cell division and increase the intracellular level of cyclic AMP in glioma and NB cells. Long-term treatment of glioma and NB cells with lower concentrations of CH 3 HgCI (0.05-0.2 µM) produces marked alteration in gene expression as evidenced by changes in the amount and phosphorylation of specific proteins [12,13], and reduces the sensitivity of adenylate cyclase to prostaglandin E 1 in glioma cells, but not in NB cells [11].…”

Section: Treatmentmentioning

confidence: 99%

Effects of methylmercuric chloride on high affinity uptake of certain putative neurotransmitters in neuroblastoma and glioma cell cultures

1979

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SUMMARYThe effect of methylmercuric chloride (CH 3 HgCl) on high affinity uptake of certain putative neurotransmitters on mouse neuroblastoma (NBE-) and rat glioma (C-6) cells in culture 3 days after treatment was studied. The energydependent accumulation of radioactive choline was decreased in NB cells after treatment with CH 3 HgCl (1 µM), whereas the accumulation of radioactive glycine or glutamate was increased. The uptake of radioactive glycine or glutamate in glioma cells was decreased after treatment with CH 3 HgCl (0.3 µM). High affinity uptake of choline was not demonstrable in glioma cells. Low concentrations of CH 3 HgCl affect the accumulation of glycine and glutamate in glioma and NB cells in an opposite manner.

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Use of Cultures of Neuroblastoma and Glioma as a Model System to Study the Heavy Metal-Induced Neurotoxicity

Prasad

1983

In Vitro Toxicity Testing of Environmental Agents

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Effects of methylmercuric chloride in the presence of cyclic AMP-stimulating agents on glioma and neuroblastoma cells in culture

Cited by 16 publications

References 16 publications

Altered Patterns of Protein Phosphorylation and Synthesis Caused by Methyl Mercury in Cerebellar Granule Cell Culture

Altered Patterns of Protein Phosphorylation and Synthesis Caused by Methyl Mercury in Cerebellar Granule Cell Culture

Effects of methylmercuric chloride on high affinity uptake of certain putative neurotransmitters in neuroblastoma and glioma cell cultures

Use of Cultures of Neuroblastoma and Glioma as a Model System to Study the Heavy Metal-Induced Neurotoxicity

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