Effects of microalgae diets and stocking density on larval growth, survival and metamorphosis of dwarf surfclam, Mulinia lateralis

Yang, Zujing; Huang, Xiaoting; Wang, Hao; Pan, Haoyuan; Wang, Xinglei; Teng, Mingxuan; Ren, Qiang; Bao, Zhenmin

doi:10.1016/j.aquaculture.2021.736440

Cited by 19 publications

(21 citation statements)

References 36 publications

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“…Previous studies have suggested that, in most cases, it is better to feed mixed microalgae rather than a single species, as they may provide more balanced nutrition (Cheng et al, 2020). At the larvae stage of M. lateralis, the mixed diet of Isochrysis galbana and Platymonas helgolandica as diet significantly improved the larval survival and growth (Yang et al, 2021). Similar studies have been reported in many species, such as Pinna nobilis (Prado et al, 2020) and M. mercenaria (Hassan et al, 2021).…”

Section: Discussionsupporting

confidence: 71%

“…These unique characteristics of M. lateralis have made it an attractive model species for bivalve researches (Calabrese, 1969a;Guo and Allen, 1994;Burgess and Morrison, 2010;Cripe, 2010). Some signs of progress have been made in the physiological ecology, reproduction, and artificial culture of M. lateralis, which laid a solid foundation for further research (Calabrese, 1969b;Shumway, 1983;Shumway et al, 1983;Shumway and Newell, 1984;Yang et al, 2021). While spawning induction and subsequent culture of M. lateralis can be achieved in the laboratory (Calabrese, 1969a;Rhodes et al, 1975), a major challenge impeding production of this species remains the lack of standardized culture protocols.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the optimization of these factors is crucial for a successful and efficient culture system. In our previous study, the rearing conditions for M. lateralis have been developed by optimizing the microalgae diet and stocking density, which greatly promoted the production of high-quality larvae under laboratory conditions (Yang et al, 2021). However, very less is known about the optimal culture conditions for M. lateralis spat, which is a key stage in the transition from juvenile to adult.…”

Section: Introductionmentioning

confidence: 99%

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Optimizing Microalgae Diet, Temperature, and Salinity for Dwarf Surf Clam, Mulinia lateralis, Spat Culture

Yang

Wang

et al. 2022

Front. Mar. Sci.

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Dwarf surf clam, Mulinia lateralis, is widely considered as a model species for bivalves. The development of a standard culture system could greatly promote the production of high-quality individuals, yet information on the culture conditions for M. lateralis spat is still limited. This study aims to determine the suitable microalgae diet, temperature, and salinity for M. lateralis spat culture. The typically fed microalgae species, including Chlorella pyrenoidesa (Cp), Platymonas helgolandica (Ph), Dunaliella salina (Ds), Nitzschia closterium (Nc), and Chaetoceros muelleri (Cm), could be taken up by M. lateralis spat, and their filtration rates on Cp, Nc, and Cm were higher than those on Ds and Ph. For the entire spat culture, all diet trials showed similar survival percentages, while the mono-specific diet Cp exhibited the highest growth rate, suggesting that Cp was the optimal microalgae species for M. lateralis spat. Through simultaneously maximizing the growth and survival of spat, the optimal microalgae concentration and stocking density were 5 × 104 cells ml–1 and 400–600 individuals m–2 for 30–40-day-old spat as well as 1 × 105 cells ml–1 and 400–600 individuals m–2 for 40–50-day-old spat, respectively. In addition, the spat had higher growth rates and survival percentages at the temperature of 20–22°C and salinity of 22–25 ppt. The results of this study provide a basis for further culture of M. lateralis spat, and the optimized conditions will be of great significance for the construction of the standard culture system of M. lateralis.

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Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimizing Microalgae Diet, Temperature, and Salinity for Dwarf Surf Clam, Mulinia lateralis, Spat Culture

Yang

Wang

et al. 2022

Front. Mar. Sci.

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“…In aquaculture, microalgae are considered an essential resource, being one of the most important food sources in the early stages of fish (Reitan et al, 1997; El‐Khodary et al, 2021), molluscs (Maquirang et al, 2020; Yang et al, 2021), crustaceans (Abd Rahman et al, 2018; Turcihan et al, 2021) and echinoderms (Castilla‐Gavilán et al, 2018; Militz et al, 2018), given their nutritional value and ability to synthesize and accumulate large amounts of essential fatty acids (Malcata et al, 2018; Patil et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Early life cycle description and effects of microalgal diets on larval and post‐larval development of the mangrove cockleAnadara tuberculosa(Sowerby, 1833)

Rodríguez-Pesantes

Reyes

Márquez

et al. 2021

Aquaculture Research

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Mangrove ecosystems host a great biodiversity of unique species contributing to overall coastal balance (Rivas & de Dios, 2018).Many edible mollusks species are increasingly being extracted from mangrove habitats without major control of fishing or resource management authorities. Along the Pacific Ocean, there are at least 15 species of the mollusk genus Anadara (Paredes et al., 2016). To our knowledge, the mangrove cockle Anadara tuberculosa is the most important species fished for human consumption (MacKenzie, 2001). Due to its high demand, natural populations have declined in these ecosystems (Pretel Monzón, 2016). The reduction in Anadara

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“…To investigate the first steps of shell formation during early developmental stages, Mulinia lateralis, which is naturally distributed in estuarine benthic and intertidal zones along the Atlantic coast from Canada to the Caribbean Sea (Walker and Tenore, 1984), was selected. M. lateralis is a typical dioecious species with a small size and short generation interval of 2 months, and artificial breeding of M. lateralis in the laboratory is easy to achieve, which provides a promising model species of bivalve studies (Guo and Allen, 1994;Cripe, 2006;Yang et al, 2021). Up to now, neither transcriptomic nor genomic information has been published in M. lateralis.…”

Section: Introductionmentioning

confidence: 99%

Full-length transcriptome analysis provides insights into larval shell formation in Mulinia lateralis

Guo

Zhao

et al. 2023

Front. Mar. Sci.

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Mollusca is the second largest animal phylum and represents one of the most evolutionarily successful animal groups. Mulinia lateralis, a small bivalve, is a promising model organism to facilitate studies of mollusc development. However, because of the lack of published genomic and transcriptomic resources, integrated research on the formation of larval shells in this species, which is a representative developmental process of molluscs and of great importance for larva survival, is hindered. In this study, the blastula, gastrula, trochophore larva, and D-shaped larva of M. lateralis were utilized for generating a comprehensive full-length transcriptome through Pacific BioSciences (PacBio) isoform sequencing (Iso-seq) and Illumina RNA-Seq. A total of 238,919 full-length transcripts with an average length of 3,267 bp and 121,424 annotated genes were obtained. Illumina RNA-Seq data analysis showed that 4,512, 10,637, and 17,829 differentially expressed genes (DEGs) were obtained between the two adjacent developmental stages. Functional annotation and enrichment analysis revealed the specific function of genes in shell biomineralization during different developmental stages. Twelve genes that may be involved in the formation of the larval shell of M. lateralis were identified, including insoluble shell matrix protein-encoding gene 1 (ISMP1), ISMP2, ISMP5, chitin synthase, tyrosinase, chitin-binding protein, collagen and pu14 involved in shell matrix deposition, and carbonic anhydrase, solute carrier family 4 member 8 (slc4a8), EF-hand, and a calmodulin coding gene C-2442 participated in ion transportation. In addition, calcium ion binding function, calcium signaling pathway, and endocrine and other factor-regulated calcium reabsorption pathways were significantly enriched. Weighted gene correlation network analysis (WGCNA) identified two modules related to biomineralization and larval shell formation, and slc4a8 and ring finger protein 41 (rnf41) were key hub genes that may be involved in this process. Moreover, it could be implied that the process of ion transport occurs earlier than the deposition of the shell matrix. This work provided a clear view of the transcriptome for M. lateralis and will be valuable in elucidating the mechanisms of larval shell formation as well as other developmental processes in molluscs.

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Effects of microalgae diets and stocking density on larval growth, survival and metamorphosis of dwarf surfclam, Mulinia lateralis

Cited by 19 publications

References 36 publications

Optimizing Microalgae Diet, Temperature, and Salinity for Dwarf Surf Clam, Mulinia lateralis, Spat Culture

Optimizing Microalgae Diet, Temperature, and Salinity for Dwarf Surf Clam, Mulinia lateralis, Spat Culture

Early life cycle description and effects of microalgal diets on larval and post‐larval development of the mangrove cockleAnadara tuberculosa(Sowerby, 1833)

Full-length transcriptome analysis provides insights into larval shell formation in Mulinia lateralis

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