Effects of microcystin-LR on the metal bioaccumulation and toxicity in Chlamydomonas reinhardtii

Wang, Ningxin; Zhang, Xueying; Wu, Jun; Xiao, Lin; Yin, Ying; Miao, Ai-Jun; Ji, Rong; Yang, Liuyan

doi:10.1016/j.watres.2011.10.035

Cited by 39 publications

(12 citation statements)

References 40 publications

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“…3b). The [Cd 2+ ] F -based EC50 thus obtained was 0.35±0.03 mg/l which was similar to 0.46 mg/l observed in our previous study [31] and was within the range of values (0.03–2.41 mg/l) reported in the literature [32], [33]. As the cell growth was differently inhibited at similar [Cd 2+ ] T but various concentrations of TiO 2 -ENs, only the dose-related responses of the first toxicity test without any addition of TiO 2 -ENs were simulated with the Logistic model in Fig.…”

Section: Resultssupporting

confidence: 90%

Cd2+ Toxicity to a Green Alga Chlamydomonas reinhardtii as Influenced by Its Adsorption on TiO2 Engineered Nanoparticles

2012

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In the present study, Cd2+ adsorption on polyacrylate-coated TiO2 engineered nanoparticles (TiO2-ENs) and its effect on the bioavailability as well as toxicity of Cd2+ to a green alga Chlamydomonas reinhardtii were investigated. TiO2-ENs could be well dispersed in the experimental medium and their pHpzc is approximately 2. There was a quick adsorption of Cd2+ on TiO2-ENs and a steady state was reached within 30 min. A pseudo-first order kinetics was found for the time-related changes in the amount of Cd2+ complexed with TiO2-ENs. At equilibrium, Cd2+ adsorption followed the Langmuir isotherm with the maximum binding capacity 31.9, 177.1, and 242.2 mg/g when the TiO2-EN concentration was 1, 10, and 100 mg/l, respectively. On the other hand, Cd2+ toxicity was alleviated in the presence of TiO2-ENs. Algal growth was less suppressed in treatments with comparable total Cd2+ concentration but more TiO2-ENs. However, such toxicity difference disappeared and all the data points could be fitted to a single Logistic dose-response curve when cell growth inhibition was plotted against the free Cd2+ concentration. No detectable amount of TiO2-ENs was found to be associated with the algal cells. Therefore, TiO2-ENs could reduce the free Cd2+ concentration in the toxicity media, which further lowered its bioavailability and toxicity to C. reinhardtii.

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Section: Resultssupporting

confidence: 90%

Cd2+ Toxicity to a Green Alga Chlamydomonas reinhardtii as Influenced by Its Adsorption on TiO2 Engineered Nanoparticles

2012

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“…The hypothesis was supported by the study of Fujii et al [ 8 ], where the uptake of both ferric and ferrous iron by M. aeruginosa PCC 7806 remains unchanged when the MC synthesis gene mcyH in this cyanobacterium is inactivated. Our previous study also manifests that 1 mg/L MC does not have any considerable roles in the bioavailability of various metals (Cd, Cu, Zn, and Cr) to the green alga C. reinhardtii [ 4 ].…”

Section: Resultsmentioning

confidence: 94%

“…Then an aliquot of 0.5 mL from each replicate was taken and acidified with ultrapure concentrated HNO 3 (final concentration, 3.5% w/v). Thereafter, its [Cd] T was measured by a graphite furnace atomic absorption spectrophotometer (GFAAS, Thermo Fisher Scientific Inc., Waltham, MA, USA) [ 4 ]. Polypropylene beakers and polycarbonate bottles were used throughout the experiment to minimize Cd loss onto the container wall.…”

Section: Methodsmentioning

confidence: 99%

“…Putative roles include metal complexation, quorum sensing, predator avoidance, and inter-cellular interaction [ 2 , 3 ]. In our previous study, MC was found not to influence the speciation of four metal ions (Cd, Cr, Cu, and Zn) in the experimental medium [ 4 ]. Under this condition, the bioavailability and toxicity of these metals to the freshwater green alga Chlamydomonas reinhardtii are still in accordance with the conventional free ion activity model (FIAM) [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Cadmium Toxicity to Microcystis aeruginosa PCC 7806 and Its Microcystin-Lacking Mutant

Bin

Miao

et al. 2015

PLoS ONE

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The adverse effects of microcystin (MC) produced by cyanobacteria have drawn considerable attention from the public. Yet it remains unclear whether MC confers any benefits to the cyanobacteria themselves. One suggested function of MC is complexation, which may influence the bioaccumulation and toxicity of trace metals. To test this hypothesis, we examined Cd toxicity to wild-type Microcystis aeruginosa PCC 7806 (WT) and its MC-lacking mutant (MT) under nutrient-enriched (+NP), phosphorus-limited (-P), and nitrogen-limited (-N) conditions. The accumulation of Cd and the biochemical parameters associated with its detoxification [total phosphorus (TP), inorganic polyphosphate (Poly-P), and glutathione (GSH) in the cells as well as intra- and extra-cellular carbohydrates] were quantified. Although the –P cyanobacteria accumulated less Cd than their +NP and –N counterparts, the different nutrient-conditioned cyanobacteria were similarly inhibited by similar free ion concentration of Cd in the medium ([Cd2+]F). Such good toxicity predictability of [Cd2+]F was ascribed to the synchronous decrease in the intracellular concentrations of Cd and TP. Nevertheless, Cd toxicity was still determined by the intracellular Cd to phosphorus ratio (Cd/P), in accordance with what has been reported in the literature. On the other hand, the concentrations of TP, Poly-P, and carbohydrates went up, but GSH concentration dropped down with the enhancement of [Cd2+]F, indicating their association with Cd detoxification. Although the inactivation of MC peptide synthetase gene had some nutrient and Cd concentration dependent effects on the parameters above, both cyanobacterial strains showed the same Cd accumulation ability and displayed similar Cd sensitivity. These results suggest that MC cannot affect metal toxicity either by regulating metal accumulation or by altering the detoxification ability of the cyanobacteria. Other possible functions of MC need to be further investigated.

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“…Heavy metal ions in industrial wastewater pose a potential threat to the living environment and human health due to their accumulation and enrichment in vivo 1 . Electrochemical approaches are especially effective for heavy metal removal but require a power supply 2 3 .…”

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confidence: 99%

Assembly of coupled redox fuel cells using copper as electron acceptors to generate power and its in-situ retrieval

Zhang

et al. 2016

Sci Rep

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Energy extraction from waste has attracted much interest nowadays. Herein, a coupled redox fuel cell (CRFC) device using heavy metals, such as copper, as an electron acceptor is assembled to testify the recoveries of both electricity and the precious metal without energy consumption. In this study, a NaBH4-Cu(II) CRFC was employed as an example to retrieve copper from a dilute solution with self-electricity production. The properties of the CRFC have been characterized, and the open circuit voltage was 1.65 V with a maximum power density of 7.2 W m−2 at an initial Cu2+ concentration of 1,600 mg L−1 in the catholyte. 99.9% of the 400 mg L−1 copper was harvested after operation for 24 h, and the product formed on the cathode was identified as elemental copper. The CRFC demonstrated that useful chemicals were recovered and the electricity contained in the chemicals was produced in a self-powered retrieval process.

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Effects of microcystin-LR on the metal bioaccumulation and toxicity in Chlamydomonas reinhardtii

Cited by 39 publications

References 40 publications

Cd2+ Toxicity to a Green Alga Chlamydomonas reinhardtii as Influenced by Its Adsorption on TiO2 Engineered Nanoparticles

Cd2+ Toxicity to a Green Alga Chlamydomonas reinhardtii as Influenced by Its Adsorption on TiO2 Engineered Nanoparticles

Cadmium Toxicity to Microcystis aeruginosa PCC 7806 and Its Microcystin-Lacking Mutant

Assembly of coupled redox fuel cells using copper as electron acceptors to generate power and its in-situ retrieval

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