Effects of mint, thyme, and curcumin extract nanoformulations on the sperm quality, apoptosis, chromatin decondensation, enzyme activity, and oxidative status of cryopreserved goat semen

Ismail, Aya A.; Abdel-Khalek, A.; Khalil, Wael A.; Yousif, Ahmed I.; Saadeldin, Islam M.; Abomughaid, Mosleh M.; El-Harairy, M.

doi:10.1016/j.cryobiol.2020.09.002

Cited by 37 publications

(28 citation statements)

References 65 publications

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“…This emphasizes the importance of supplementing the freezing media with added antioxidants that would minimize the negative effect of ROS and maintain the quality of postthawed sperm (13). In this regard, different types of supplements are added to the freezing media as protective agents; however, antioxidants of natural origin are of special interest in this concern (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

A Polyphenol-Rich Extract From Entada abyssinica Reduces Oxidative Damage in Cryopreserved Ram Semen

Sobeh

Hassan

Hassan³

et al. 2020

Front. Vet. Sci.

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The Splinter bean, Entada abyssinica, is widely used in folk medicine. In the current work, we profiled the secondary metabolites from E. abyssinica bark extract using LC-MS and investigated its effect on cryopreserved ram semen. Twenty-eight compounds, including tannins and gallic acid derivatives that prevailed in the extract, were tentatively identified. Results showed that the quality of the post-thawed semen showed a significant improvement when the extract was added to the extender at a concentration of 375 μg/mL. The progressive motility and plasma membrane integrity of sperm cells were significantly increased in the post-thawed semen; however, the total antioxidant capacity (TAC) was insignificantly increased. A significant decrease in the concentration of hydrogen peroxide was detected as well. No significant changes were observed in activities of lactate dehydrogenase (LDH), alanine aminotransaminase (ALT), and aspartate transaminase (AST) within the treated samples. Intact sperm percentage was significantly increased, while apoptotic and necrotic sperm percentages were reduced significantly. Molecular docking of some individual components from the extract revealed their potential to interfere with the apoptosis cascade in which Bcl-2 is involved. In conclusion, Entada abyssinica appears to be useful for cryopreservation presumably owing to its polyphenol content that has potent antioxidant capacity scavenging reactive oxygen species (ROS), enhancing the endogenous antioxidant system and inhibiting lipid peroxidation.

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Section: Introductionmentioning

confidence: 99%

A Polyphenol-Rich Extract From Entada abyssinica Reduces Oxidative Damage in Cryopreserved Ram Semen

Sobeh

Hassan

Hassan³

et al. 2020

Front. Vet. Sci.

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Section: Discussionmentioning

confidence: 99%

“…Moreover, higher percentages of sperm with intact membranes were observed in the quercetin-supplemented sperm samples than in the control samples. The greater membrane integrity of quercetin-supplemented sperm is an indicator of preserved sperm structure and ultimately the enhanced survival rate of sperm(Ismail et al 2020).Sperm are very sensitive to oxidative stress owing to their limited oxidative defense mechanisms (Alahmar 2019,Sabeti et al 2016). The sensitivity to oxidative stress is further ampli ed by the dilution of semen antioxidantive enzymes during semen dilution process and excessive ROS generation during post-thaw (Bansal &Bilaspuri 2010).…”

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confidence: 99%

Quercetin Improves the Apoptotic Index and Oxidative Stress in Post-Thaw Dog Sperm

Bang

Qamar

Tanga

et al. 2021

Preprint

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This study was conducted to investigate if quercetin (QRN) may ameliorate apoptosis and oxidative stress in post-thaw dog sperm. Herein, we evaluated the post-thaw apoptosis and oxidative stress after treatment with QRN (control, 25, 50, and 100 µM) in freezing of dog semen. The oxidative stress index was significantly affected (p<0.05) between the various concentrations of QRN and the control (17.56 ± 1.02, 7.54 ± 0.48, 5.66 ±0.80, and 10.41 ± 0.69), respectively. The apoptosis index was 9.1 ± 1.34, 6.66 ± 0.58, 6.77 ± 0.66, and 5.38 ± 0.86 in the control, and 25, 50, and 100 µM QRN treatment groups, respectively (p< 0.05). The effects of ameliorated cryo-induced damage by QRN on post-thaw sperm quality were also observed through improved structural and functional tests. Sperm treated with 50 µM QRN showed significantly higher motility (51.8 ± 2.1% vs. 43.1 ± 1.4%, P < 0.05), survival rates (46.9 ± 0.7% vs. 43.9 ± 0.4%, P < 0.05), and mucus penetration than control group, respectively. Results demonstrate that supplementing freezing buffer with 50 µM QRN reduced oxidative damage and improved the quality of post-thaw dog sperm.

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“…The citrate–egg yolk extender containing SOD and butylated hydroxytoluene reduce the production of MDA in bovine semen post‐cryopreservation (Asadpour et al., 2012). Fresh bull semen supplemented with curcumin significantly increases GSH content post‐thawing (Shokry et al., 2020), its nanoformulations potentiate sperm function, decrease sperm apoptosis and chromatin decondensation of post‐thawed buck semen (Ismail et al., 2020). Further, the high content of antioxidants, flavonoids, vitamins, organic acids, pollen grains and honey protects bull semen (El‐Sheshtawy et al., 2014; El‐Sheshtawy et al., 2014).…”

Section: Antioxidant Status and Counteracting Oxidative Stressmentioning

confidence: 99%

Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview

et al. 2021

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Sperm cryopreservation is an important adjunct to assisted reproduction techniques (ART) for improving the reproductive efficiency of dairy cattle and buffaloes. Improved understanding of mechanisms and challenges of bovine semen cryopreservation is vital for artificial insemination on a commercial basis. Although cryopreservation of bovine spermatozoa is widely practiced and advanced beyond that of other species, there are still major gaps in the knowledge and technology. Upon cryopreservation, disruption of spermatozoal plasma membrane configuration due to alterations in metabolic pathways, enzymes and antioxidants activity add to lower efficiency with loss of sperm longevity and fertilising ability. Therefore, the effective amalgamation of cryo‐variables like ambient temperature, cooling and thawing rates, nucleation temperature, type and concentration of the cryoprotectant, seminal plasma composition, free radicals and antioxidant status are required to optimise cryopreservation. Novel strategies like supplementation of cholesterol‐loaded cyclodextrins (CLC), nanovesicles, osteopontin, antioxidants, etc., in an extender and recent techniques like nano‐purification and modified packaging have to be optimised to ameliorate the cryodamage. This article is intended to describe the basic facts about the sperm cryopreservation process in bovine and the associated biochemical, biophysical, ultra‐structural, molecular and functional alterations.

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Effects of mint, thyme, and curcumin extract nanoformulations on the sperm quality, apoptosis, chromatin decondensation, enzyme activity, and oxidative status of cryopreserved goat semen

Cited by 37 publications

References 65 publications

A Polyphenol-Rich Extract From Entada abyssinica Reduces Oxidative Damage in Cryopreserved Ram Semen

A Polyphenol-Rich Extract From Entada abyssinica Reduces Oxidative Damage in Cryopreserved Ram Semen

Quercetin Improves the Apoptotic Index and Oxidative Stress in Post-Thaw Dog Sperm

Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview

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