Effects of miRNA-140 on the Growth and Clinical Prognosis of SMMC-7721 Hepatocellular Carcinoma Cell Line

Kong, Cunqing; Chen, Xingcai; Qiu, Guanhua; Liang, Jingchen; Wang, Duo; Liu, Xinyu; Liu, Jun-Jie; Han, Yaoqi; Fan, Xiao

doi:10.1155/2021/6638915

Cited by 7 publications

(3 citation statements)

References 41 publications

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“…With the development of molecular biology, the detection of biomarkers can provide an effective assessment of the prognosis of LC. e expression of miRNA is related to the growth, proliferation, migration, and other processes of tumor cells and affects the occurrence and prognosis of many diseases including tumors [22][23][24]. Many studies are limited to the clinical analysis of patients with SP and LC, while ignoring the importance of clinical diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Study on Serum miR-204 Expression Levels in Patients with Severe Pneumonia and Patients with Primary Bronchial Lung Cancer and Its Diagnostic Value

Zheng¹,

Huang

2021

Evidence-Based Complementary and Alternative Medicine

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Objective. To analyze the expression and clinical significance of miR-204 in the serum of patients with severe pneumonia (SP) and primary bronchial lung cancer (LC). Methods. 65 SP patients and 43 primary bronchial LC patients who were treated in the hospital from January 2017 to December 2018 were randomly selected as the SP group and LC group. At the same time, healthy patients from the physical examination department of the hospital were selected. 65 cases were the control group. QRT-PCR detected serum miR-204 expression and compared the differences between groups. The pathological data of patients were collected, and the relationship between serum miR-204 and the patient’s pathological data was compared; the area under the ROC curve and Kaplan–Meier curve were used to evaluate the diagnostic value of serum miR-204 for the two conditions and to explore the relationship between serum miR-204 and prognosis. Results. The serum miR-204 of the SP group was (0.43 ± 0.09), the serum miR-204 of the LC group was (0.40 ± 0.10), the serum miR-204 of the control group was (1.00 ± 0.09), and the miR-204 level of was significantly higher than that of the control group, and the difference between the groups was statistically significant ( P < 0.05). There was no significant difference in serum miR-204 levels between the SP group and the LC group ( P > 0.05). Serum miR-204 levels in SP patients with cumulative organs ≥3 were higher than those with cumulative organs <3, and the difference was statistically significant ( P < 0.001). In the LC group, in patients with stage III to IV and low and undifferentiated patients, the level of miR-204 was higher than that of stage I∼II and high and moderately differentiated patients, and the difference was statistically significant ( P < 0.001). The level of miR-204 in the two groups of patients (0.89 ± 0.10, 0.83 ± 0.13) who died of illness was significantly higher than that of the surviving patients (1.00 ± 0.11, 1.00 ± 0.10), and the difference was statistically significant ( P < 0.05); the survival rate of patients with high expression of miR-204 was higher than that of patients with low expression. The AUC of serum miR-204 level to SP and LC was 0.766 and 0.818, respectively. Conclusion. The level of miR-204 in the serum of SP patients and patients with primary bronchial LC was significantly lower than that of healthy people, and patients who died were lower than those who survived; the miR-204 in serum has a good diagnostic value for SP and LC and is related to the survival and prognosis of patients.

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Section: Discussionmentioning

confidence: 99%

Study on Serum miR-204 Expression Levels in Patients with Severe Pneumonia and Patients with Primary Bronchial Lung Cancer and Its Diagnostic Value

Zheng¹,

Huang

2021

Evidence-Based Complementary and Alternative Medicine

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“…Various researches indicated that abnormal expression of lncRNAs is highly correlated to the prognosis and metastasis of HCC [ 5 , 6 ]. MicroRNA (miRNA) is a class of endogenous, single-stranded, non-coding RNAs of 19–25 nt length [ 7 ], in which its prominent role is to bind to the 3’ untranslated region of its target mRNA, thereby inhibiting gene expression [ 8 ]. The interactions of mRNA, miRNA, and lncRNA play multiple roles in the development of HCC.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of GSEC/miR-101-3p/SNX16/PAPOLG axis in hepatocellular carcinoma

Zhang

Guo

et al. 2022

PLoS ONE

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Hepatocellular carcinoma (HCC) is one of the most lethal malignancies. A growing number of studies have shown that competitive endogenous RNA (ceRNA) regulatory networks might play important roles during HCC process. The present study aimed to identify a regulatory axis of the ceRNA network associated with the development of HCC. The roles of SNX16 and PAPOLG in HCC were comprehensively analyzed using bioinformatics tools. Subsequently, the “mRNA-miRNA-lncRNA” model was then used to predict the upstream miRNAs and lncRNAs of SNX16 and PAPOLG using the miRNet database, and the miRNAs with low expression and good prognosis in HCC and the lncRNAs with high expression and poor prognosis in HCC were screened by differential expression and survival analysis. Finally, the risk-prognosis models of ceRNA network axes were constructed by univariate and multifactorial Cox proportional risk analysis, and the immune correlations of ceRNA network axes were analyzed using the TIMER and GEPIA database. In this study, the relevant ceRNA network axis GSEC/miR-101-3p/SNX16/PAPOLG with HCC prognosis was constructed, in which GSEC, SNX16, and PAPOLG were highly expressed in HCC with poor prognosis, while miR-101-3p was lowly expressed in HCC with good prognosis. The risk-prognosis model predicted AUC of 0.691, 0.623, and 0.626 for patient survival at 1, 3, and 5 years, respectively. Immuno-infiltration analysis suggested that the GSEC/miR-101-3p/SNX16/PAPOLG axis might affect macrophage polarization. The GSEC/miR-101-3p/SNX16/PAPOLG axis of the ceRNA network axis might be an important factor associated with HCC prognosis and immune infiltration.

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“…HCC cell lines are powerful tools. Until now, ten human HCC cell lines have been established, and every cell line has its own characteristics and offers convenience for various experiments [16][17][18][19]. Stability, homogeneity and straightforward culture are important parameters permanently in cell lines.…”

Section: Medicinal Plantmentioning

confidence: 99%

Evaluation of Anti Proliferative Potential of Solanum virginianum Human Hepatocellular Carcinoma Cell Line

Selvapriya

Arivarasu

Subramanian

et al. 2021

JPRI

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Introduction: Solanum virginianum commonly known as wild eggplant or nightshade plant, is a prickly herb found in most of the parts of Asia and Australia of the world. It is used by the local people as folk medicines in treating throat infections and other inflammatory problems. Various phytoconstituents have been found, the major constituent is alkaloid. It has a vital role in various traditional as well as medicinal uses for curing internal and external physiological disorders. To determine the evaluation of anti proliferation potential of Solanum virginianum human hepatocellular carcinoma cell line. Materials and Methods: The effect of solanum virginianum hepG2 cell viability was measured by MTT assay. Briefly, the cells (1 × 104 cells per mL) were seeded in a 96 well microtiter plate (100 per well) with replications. Treatment was conducted for 24 hr with different concentrations (50, 100, 150, 200, 250, 300 μM) of solanum virginianum. Cell morphological changes were observed in phase contrast microscopy. Results: Cells were treated with solanum virginianum at higher concentration (50, 75, 100, 200, 300 and 400 μM) for 24 h, and cell viability was evaluated by MTT assay. Data are shown as means ± SD (n = 3). * compared with the control-blank group, p < 0.001. Conclusion: From the results, the extracts were cytotoxic to the hepG2 cells at (60μg/mL) concentration and incubation period. However more research is needed to understand the mechanisms of cytotoxicity of the plants.

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Effects of miRNA-140 on the Growth and Clinical Prognosis of SMMC-7721 Hepatocellular Carcinoma Cell Line

Cited by 7 publications

References 41 publications

Study on Serum miR-204 Expression Levels in Patients with Severe Pneumonia and Patients with Primary Bronchial Lung Cancer and Its Diagnostic Value

Study on Serum miR-204 Expression Levels in Patients with Severe Pneumonia and Patients with Primary Bronchial Lung Cancer and Its Diagnostic Value

Comprehensive analysis of GSEC/miR-101-3p/SNX16/PAPOLG axis in hepatocellular carcinoma

Evaluation of Anti Proliferative Potential of Solanum virginianum Human Hepatocellular Carcinoma Cell Line

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