Effects of Mitomycin C on Human Chromosomes

LOCALIZED CHROMOSOME BREAKAGE INDUCED BY MITOMYCIN C IN <i>TRADESCANTIA PALUDOSA</i> AND <i>VICIA FABA</i> ROOT TIPS

“…Similar localization has been observed for MC by Merz (1961) and Rao and Natarajan (1967) in Vicia, as well as by Cohen and Shaw (1964) and Nowell (1964) in human leucocyte cultures.…”

“…MC has been reported on several occasions to cause mitotic inhibition as well as chromosome breakage. The chromosome aberrations induced by MC were also localized in heterochromatic regions in Vicia (Merz 1961) and secondary constrictions in cultured cells of human leucocytes (Cohen and Shaw 1964;Nowell 1964).…”

Genotixicity of Mitocycin C – Cytogenetic Study

“…Cohen MM et al (1964) 7 found that 50% total exchanges induced by mitomycin c were homologus and selective for chromosome 1 and 9 and Morad M (1973) 27 found an over representation of interchange aberrations involving the C-bands of homologous chromosomes especially the secondary constrictions of chromosomes, 1, 9 and 16. Observation of the present study was fully correlated with the findings of Tchun TS et al (1976) 9 who observed that more than half of aberration induced by mitomycin c were interchange breakage and 60 to 70% of which involve the secondary constriction region.…”

“…Cohen MM and Shaw MW (1964) 7 found maximum chromosomal breakages at concentration 1.0 µg/ml of Mitomycin C for 24 hour exposure. 8 used a same concentration and time to treat human leucocytes but failed to produce chromosome breaks.…”

“…Cohen MM et al (1964) 12 found that Chromosome 1, 9 and 16 possessing prominent secondary constrictions, appeared to be focal points of mitomycin c activity. and chromosome 9 was most commonly involved chromosome in chromosomal aberrations.…”