Effects of Modifiers on the Optical Properties of Oxidized Cholesterol Model Membranes

Abstract

Brewster angle reflections from oxidized cholesterol membranes are described in terms of uniaxial crystal model. The refractive indices perpendicular and parallel to the membrane are 1.515 and 1.555, respectively. A multilayer model was also considered; however, under the approximations used, both models are equivalent and cannot be distinguished. Egg albumin and hexadecyltrimethylammonium bromide altered the refractive indices while 2,4-dinitrophenol and valinomycin addition did not produce a detectable change.

Optical properties of ovalbumin in 0.130–2.50 μm spectral region

“…A number of researchers investigated the optical properties of ovalbumin 3, 6–11. Many of these studies concentrated on microwave measurements 3, 6–8.…”

“…Many of these studies concentrated on microwave measurements 3, 6–8. Measurements in the UV‐visible– near‐IR region of the electromagnetic spectrum determined the real part ( n ) of the complex refractive index at a small number of discrete wavelengths 9–11. There were no attempts to obtain accurate values of n and k for ovalbumin throughout the UV‐visible–near‐IR region.…”