The experiment was carried out during 2015 and 2016 seasons on 20 years old Picual olive trees and grown in a sandy soil at experimental Farm of Faculty of Environmental Agricultural Sciences, Arish University, North Sinai Governorate, Egypt, to examine the effects of aqueous extracts of Athir (Artemisia monosperma), Moringa (Moringa oleifera) and Kabbar (Capparis spinosa) at four concentrations (0, 5, 15, and 25%) with Protamine (the commercial form of amino acids mixture) at 1.5 % on growth, leaf nutrient contents, productivity, fruit quality and oil properties of "Picual" olive trees. Fresh leaves were collected, washed with tap water, chopped and pounded, soaked in distilled water and filtered to prepare extracts at 5,15 and 25%. Plant extracts were sprayed three times at 70% full-bloom, after fruit set, and a month later. A control experiment with distilled water was also set up. Treated olive trees were arranged as a factorial experiment in a randomized complete block design with three replicates, each replicate was represented by two trees. The obtained results indicated that, most plant extracts with amino acid treatments significantly increases vegetative growth (shoot length, number of leaves, leaf area, leaf pigment contents, leaf chemical constituents), fruit yield, fruit physical and chemical properties, as well as oil production compared with the control. Treatments with A. monosperma or M. oleifera at 25% with/out 1.5% amino acid (Protamine) were the most effective ones compared with the other treatments.