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Taking Indian Moringa oleifera as the object, using pot experiment, setting different nitrogen application levels under the mowing conditions, the most appropriate nitrogen fertilizer application was determined by analysing the effects of different nitrogen application levels on the dry matter and uptake of nitrogen, phosphorus and kalium. The results showed that after mowing five times, when the application rates of phosphorus and kalium and are both 250mg kg-1, the yield of Moringa oleifera leaves increased significantly with the increase of nitrogen application rate. The highest yield was 595.67 g /tree when the nitrogen application rate is 300 mg kg-1. It is favorable for dry matter accumulation of Moringa oleifera, especially leaf dry matter when the nitrogen application rate is 300mg N / kg, and increases its absorption of phosphorus and potassium. Therefore, in the present experiment condition, when the dosage of nitrogen, phosphorus and potassium in the cultivation of Moringa oleifera is 300 mg kg-1, 250 mg kg-1,250 mg kg-1 respectively, it was beneficial to improve the yield and nutrient uptake of Moringa oleifera.