Effects of multicycle gonadotropin-releasing hormone antagonist protocols on oxidative stress of follicular fluid and ovarian granulosa cells

Ma, Yucong; Zhao, Zhiming; Hao, Guimin; Cui, Na; Fan, Yanli; Cao, Yucong; Tan, Zhanwang; Geng, Jingran; Liu, Fan; Du, Hongyin; Gao, Bu-Lang

doi:10.1007/s13577-021-00545-9

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…Shapiro et al (2001) observed that pregnancy and embryo implantation rates decreased significantly after repeated COH. Our previous clinical studies indicated that repeated COH results in oxidative stress and changes the follicle microenvironment, possibly causing poor IVF outcomes in patients (Ma et al 2021). In the present study, we also found that oxidative stress affects ovarian and follicular morphology, the PB1 discharge rate, and the pregnancy rate in mice (Fig.…”

Section: Discussionsupporting

confidence: 79%

-Acetylcysteine improves oocyte quality through modulating the Nrf2 signaling pathway to ameliorate oxidative stress caused by repeated controlled ovarian hyperstimulation

Liu

Guan

et al. 2022

Reprod. Fertil. Dev.

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Context N-acetyl-cysteine (NAC) is a potent antioxidant that can be used for many gynecological diseases such as polycystic ovary syndrome and endometriosis. Controlled ovarian hyperstimulation (COH) is a critical step in infertility treatment. Our previous clinical studies have shown that repeated COH led to oxidative stress in follicle fluid and ovarian granulosa cells. Aims In this study, we investigated whether NAC could inhibit oxidative stress in mice caused by repeated COH and improve the mitochondrial function of oocytes. Methods Female Institute of Cancer Research (ICR) mice were randomly assigned into three groups: normal group, model (repeated COH) group, NAC group. We examined the morphology, number and quality of mitochondria. The mechanism of regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) by NAC to ameliorate oxidative stress was also investigated. Key results Repeated COH caused oxidative damage in ovaries and oocytes and decreased oocyte quality, while NAC prevented oxidative damage and increased oocyte mitochondrial function. In in vitro experiments, it was verified that NAC can promote the nuclear translocation of Nrf2, which transcriptionally activates the expression of superoxide dismutase and glutathione peroxidase, which removed excessive reactive oxygen species that causes mitochondria damage. Conclusions The results suggest that NAC raises mitochondrial function in oocytes and improves oocyte quality through decreasing oxidative stress in mice with repeated COH. The underlying mechanism is related to the regulation of the Nrf2 signaling pathway. Implication This study provides a meaningful foundation for the future clinical application of NAC during repeated COH.

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Section: Discussionsupporting

confidence: 79%

-Acetylcysteine improves oocyte quality through modulating the Nrf2 signaling pathway to ameliorate oxidative stress caused by repeated controlled ovarian hyperstimulation

Liu

Guan

et al. 2022

Reprod. Fertil. Dev.

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“…Higher markers of OS damage and lower antioxidant enzymes in the ovaries could be observed in cycles 3–5 when compared to cycles 1–2. This was accompanied by lower quality embryo rates, implantation rates and clinical pregnancy rates [ 135 ].…”

Section: Factors Related To Os That Lead To Ovarian Agingmentioning

confidence: 99%

The role of oxidative stress in ovarian aging: a review

et al. 2022

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Ovarian aging refers to the process by which ovarian function declines until eventual failure. The pathogenesis of ovarian aging is complex and diverse; oxidative stress (OS) is considered to be a key factor. This review focuses on the fact that OS status accelerates the ovarian aging process by promoting apoptosis, inflammation, mitochondrial damage, telomere shortening and biomacromolecular damage. Current evidence suggests that aging, smoking, high-sugar diets, pressure, superovulation, chemotherapeutic agents and industrial pollutants can be factors that accelerate ovarian aging by exacerbating OS status. In addition, we review the role of nuclear factor E2-related factor 2 (Nrf2), Sirtuin (Sirt), mitogen-activated protein kinase (MAPK), protein kinase B (AKT), Forkhead box O (FoxO) and Klotho signaling pathways during the process of ovarian aging. We also explore the role of antioxidant therapies such as melatonin, vitamins, stem cell therapies, antioxidant monomers and Traditional Chinese Medicine (TCM), and investigate the roles of these supplements with respect to the reduction of OS and the improvement of ovarian function. This review provides a rationale for antioxidant therapy to improve ovarian aging.

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“…MDA is an indirect indicator of oxidative damage in human body. 39 As one of the messengers affecting sperm function, low levels of ROS can regulate sperm function, promote sperm capacitation, and regulate sperm maturation during the process from testis to egg. 40 that the ability of sperm to bind to zona pellucida could be enhanced by low levels of ROS.…”

Section: Discussionmentioning

confidence: 99%

“…SOD is an important antioxidant enzyme in the body, which can scavenge excessive free radicals released in the body and reduce the damage of cell membrane. MDA is an indirect indicator of oxidative damage in human body 39 . As one of the messengers affecting sperm function, low levels of ROS can regulate sperm function, promote sperm capacitation, and regulate sperm maturation during the process from testis to egg 40 .…”

Section: Discussionmentioning

confidence: 99%

The role of stimulator of interferon genes‐mediated AMPK/mTOR/P70S6K autophagy pathway in cyfluthrin‐induced testicular injury

Zhao

Xie

et al. 2022

Environmental Toxicology

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Cyfluthrin is widely used in the field of sanitary pest control by its wide insecticidal spectrum, high efficiency and low toxicity, low residue, and good biodegradability. But, as a double‐edged sword, a large amount of cyfluthrin remains are still in the environment. The residual cyfluthrin is absorbed into the food chain through vegetation and then poses a risk to soil organisms and human health. Several studies have suggested that cyfluthrin is one of the main factors causing testicular damage, but the mechanism remains unclear. In this study, we established in vivo and in vitro models of testicular injury in rats and GC‐2 cells exposed to cyfluthrin to explore whether stimulator of interferon genes (STING) gene mediates the regulation of AMPK/mTOR/p70S6K autophagy pathway, which lays a foundation for further study of the mechanism of testicular injury induced by cyfluthrin. The results showed that the activity of super oxide dismutase in testis decreased and the activity of malonic dialdehyde increased with the increase of concentration in vivo and in vitro. At the same time, the levels of mitochondrial damage and inflammation in the testis also increased, which further activated autophagy. In this process, the increased level of inflammation is related to the increased expression of STING gene, and AMPK/mTOR/p70S6K autophagy pathway is also involved. To sum up, cyfluthrin has certain reproductive toxicity, and long‐term exposure can induce testicular cell damage. STING gene can participate in cyfluthrin‐induced testicular injury through AMPK/mTOR/P70S6K autophagy pathway.

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Effects of multicycle gonadotropin-releasing hormone antagonist protocols on oxidative stress of follicular fluid and ovarian granulosa cells

Cited by 6 publications

References 42 publications

-Acetylcysteine improves oocyte quality through modulating the Nrf2 signaling pathway to ameliorate oxidative stress caused by repeated controlled ovarian hyperstimulation

-Acetylcysteine improves oocyte quality through modulating the Nrf2 signaling pathway to ameliorate oxidative stress caused by repeated controlled ovarian hyperstimulation

The role of oxidative stress in ovarian aging: a review

The role of stimulator of interferon genes‐mediated AMPK/mTOR/P70S6K autophagy pathway in cyfluthrin‐induced testicular injury

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