Effects of n-butanol on barley microspore embryogenesis

Effect of n-butanol and cold pretreatment on the cytoskeleton and the ultrastructure of maize microspores when cultured in vitro

“…As reported in barley microspore culture, n-butanol may act in a genotype-and pretreatmentdependent manner (Castillo et al 2014). In these experiments, n-butanol increased the number of embryos and green plants in low-responding cultivars, but not in the studied, medium and high responding cultivars.…”

“…While long exposures to n-butanol effectively slows or even blocks growth and development in Arabidopsis seedlings (Gardiner et al 2003;Motes et al 2005), shorter treatments with similar concentration resulted in elevated androgenic induction in wheat (Soriano et al 2008;Broughton 2011), maize (Földesiné Füredi et al 2011) and barley (Castillo et al 2014) microspore and anther cultures. As reported in barley microspore culture, n-butanol may act in a genotype-and pretreatmentdependent manner (Castillo et al 2014).…”

The effect of copper on plant regeneration in barley microspore culture

“…It is well documented that in barley anther culture additional Cu 2+ supplementation during both the pretreatment and the induction phase of androgenesis improves GP regeneration (Jacquard et al 2009). The IMC, despite relying on essentially the same process as AC, may react differently to the same compounds added into the media (Ritala et al 2001;Wojnarowiez et al 2002, Castillo et al 2014. This is the main reason why it appeared reasonable to verify whether the positive reaction to increased Cu 2+ in the medium observed in AC can also be repeated in IMC.…”

Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)

“…Indeed, an unprecedentedly large number of beneficial alleles should be present within the paircross parents, since they originate from a recurrent selection programme to improve androgenicity (Niels Roulund, personal communication; Andersen et al 1997). Further increases in production efficiency and yield may be achieved by utilizing recent novel understanding of microspore embryogenesis processes (Żur et al 2014;Fábián et al 2015;Sinha and Eudes 2015) and progress in optimization of (pre-)culture conditions in other cereal species such as barley, wheat or triticale (Castillo et al 2014;Würschum et al 2015;Echá-varri and Cistué 2016). Genotypes with a uniquely high androgenic capacity could be selected in the present study (Table 1) and can now be used for the introgression of this trait into recalcitrant germplasm (Halberg et al 1990;Madsen et al 1995).…”