The effect of copper on plant regeneration in barley microspore culture

Makowska, Katarzyna; Oleszczuk, Sylwia; Zimny, J.

doi:10.17221/82/2016-cjgpb

Cited by 21 publications

(16 citation statements)

References 31 publications

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“…AGPs have not been noted as involved in plastid development so the absence of an effect on albinism was not unexpected. This also provides further evidence that the development of embryos converting into the plants and chloroplast biogenesis during androgenesis are two independent processes (Chaudhary et al 2003;Makowska et al 2017).…”

Section: Discussionmentioning

confidence: 56%

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Arabinogalactan proteins improve plant regeneration in barley (Hordeum vulgare L.) anther culture

Makowska

Kaluzniak

Oleszczuk

et al. 2017

Plant Cell Tiss Organ Cult

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Section: Discussionmentioning

confidence: 56%

“…Both have high androgenic potential but with a rather low level of plant regeneration and high proportion of APs (Makowska et al 2017). Seeds were germinated on moistened filter paper in Petri dishes for 5 days at 4 °C and then for 7 days at 16 °C in the dark.…”

Section: Plant Materialsmentioning

confidence: 99%

Arabinogalactan proteins improve plant regeneration in barley (Hordeum vulgare L.) anther culture

Makowska

Kaluzniak

Oleszczuk

et al. 2017

Plant Cell Tiss Organ Cult

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“…The caryopses were sterilized by soaking in 70% ethanol for 1 min, followed by stirring in 10% sodium hypochlorite for 20 min. The sterilized caryopses were rinsed several times with sterile water ( Makowska et al, 2017 ) and used for immature zygotic embryo extraction. The extracted immature zygotic embryos were placed in Petri dishes (60 × 15 mm) containing solid N6L medium comprising macro- and micronutrients ( Chu, 1978 ) and supplemented with 2 mg l –1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 mg l –1 6-naphthalene acetic acid (NAA), and 0.5 mg l –1 kinetin.…”

Section: Methodsmentioning

confidence: 99%

Copper Ions Induce DNA Sequence Variation in Zygotic Embryo Culture-Derived Barley Regenerants

2021

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In vitro tissue culture could be exploited to study cellular mechanisms that induce sequence variation. Altering the metal ion composition of tissue culture medium affects biochemical pathways involved in tissue culture-induced variation. Copper ions are involved in the mitochondrial respiratory chain and Yang cycle. Copper ions may participate in oxidative mutations, which may contribute to DNA sequence variation. Silver ions compete with copper ions to bind to the complex IV subunit of the respiratory chain, thus affecting the Yang cycle and DNA methylation. The mechanisms underlying somaclonal variation are unknown. In this study, we evaluated embryo-derived barley regenerants obtained from a single double-haploid plant via embryo culture under varying copper and silver ion concentrations and different durations of in vitro culture. Morphological variation among regenerants and the donor plant was not evaluated. Methylation-sensitive Amplified Fragment Length Polymorphism analysis of DNA samples showed DNA methylation pattern variation in CG and CHG (H = A, C, or T) sequence contexts. Furthermore, modification of in vitro culture conditions explained DNA sequence variation, demethylation, and de novo methylation in the CHG context, as indicated by analysis of variance. Linear regression indicated that DNA sequence variation was related to de novo DNA methylation in the CHG context. Mediation analysis showed the role of copper ions as a mediator of sequence variation in the CHG context. No other contexts showed a significant sequence variation in mediation analysis. Silver ions did not act as a mediator between any methylation contexts and sequence variation. Thus, incorporating copper ions in the induction medium should be treated with caution.

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“…As explants cultured using both protocols were incubated in the same types of culture vessels and the same tissue culture chamber, our evidence of the WU protocol being more promotive of albino regeneration suggested that medium composition played a role in this response. Previous studies have shown that inclusion of casein hydrolysate reduced albinism during in vitro regeneration [66], while the use of maltose as a carbohydrate source [64], TDZ as a cytokinin source [72] and copper supplements [73] promoted albinism. While the WU protocol was more supportive of overall plantlet regeneration than LG, this protocol contained almost 8-fold higher levels of copper sulfate in both CIM and RM formulations, as well as the use of maltose in CIM, and TDZ in RM.…”

Section: Albino Regenerationmentioning

confidence: 99%

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

Flinn

Dale

Disharoon

et al. 2020

Plants

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Sorghum has been considered a recalcitrant plant in vitro and suffers from a lack of regeneration protocols that function broadly and efficiently across a range of genotypes. This study was initiated to identify differential genotype-in vitro protocol responses across a range of bioenergy sorghum parental lines and the common grain sorghum genotype Tx430 in order to characterize response profiles for use in future genetic studies. Two different in vitro protocols, LG and WU, were used for comparisons. Distinct genotype-protocol responses were observed, and the WU protocol performed significantly better for plantlet regeneration. Most bioenergy genotypes performed as well, if not better than Tx430, with Rio and PI329311 as the top regenerating lines. Genotypes displayed protocol-dependent, differential phenolic exudation responses, as indicated by medium browning. During the callus induction phase, genotypes prone to medium browning exhibited a response on WU medium which was either equal or greater than on LG medium. Genotype- and protocol-dependent albino plantlet regeneration was also noted, with three of the bioenergy genotypes showing albino plantlet regeneration. Grassl, Rio and Pink Kafir were susceptible to albino plantlet regeneration, with the response strongly associated with the WU protocol. These bioenergy parental genotypes, and their differential responses under two in vitro protocols, provide tools to further explore and assess the role of genetic loci, candidate genes, and allelic variants in the regulation of in vitro responsiveness in sorghum.

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The effect of copper on plant regeneration in barley microspore culture

Cited by 21 publications

References 31 publications

Arabinogalactan proteins improve plant regeneration in barley (Hordeum vulgare L.) anther culture

Arabinogalactan proteins improve plant regeneration in barley (Hordeum vulgare L.) anther culture

Copper Ions Induce DNA Sequence Variation in Zygotic Embryo Culture-Derived Barley Regenerants

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

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