Effects of N-Methylacetamide on the Viability, Fertility and Hatchability of Cryopreserved Ogye (Korean Native Black Fowl) Semen

Abstract

The use of methylacetamide (MA) as a cryoprotective agent for freezing Korean Native Black rooster Ogye semen was examined with artificial insemination. The diluted Ogye semen with HS-1 was subjected for 2 step dilution method of cryopreservation in which the final concentration of MA was adjusted to 7.5%. The sperm viability after thawing was reduced from 95.17 ± 0.93% to 55.93 ± 1.38% which was confirmed by live-death analysis based on Fluorescence-Activated Cell Sorting (FACS). The rates of fertilized eggs with fresh or frozen-thawed semen were reduced from 94.98 ± 3.93% to 66.36 ± 8.43% at day 7 with significant difference. However, the hatching rates of experiments at day 21 did not shown difference between 92.64 ± 2.33% and 90.45 ± 8.05% (P<0.05). With these results, the utilization of MA for freezing of Ogye spermatozoa could affect on viability of frozen-thawed semen but not on the fertility of lain eggs and hatchability of fertilized eggs and also provide possible tools of freezing for poultry genetic resource conservation.

Cryopreservation of Rooster Semen Using N-Methylacetamide as Cryoprotective Agent

“…In the present study, sperm viability was similar among the three different concentrations of MA used. The present study findings were in agreement with that of the other reports where there was significant reduction in live sperm after cryopreservation [15][16][17]. A study reported that semen cryopreservation resulted in 34% and 30% sperm viability for 9% and 12% MA concentrations in Korean Oge chicken [15].…”

“…In our study with use of 9%MA there was no fertile eggs. The result is in contrary to other reports where MA was used as cryoprotectant [7,10,[15][16][17]25]. However, the high fertility (>70%) reported in some studies [7,10] could not be replicated in other studies where different breeds of chicken were utilized [15][16][17].…”

Sperm motility and viability of experimental animals using different cryopreservatives

“…A variety of CPVs have been developed and are being utilized in different types of subjects including humans, experimental animals, livestock, companion animals, and others. CPVs are most commonly applied to livestock such as cows (Guthrie et al, 2002;Magnusson et al, 2008;Blässe, et al 2012), swine (Kwon et al, 2004;Choi et al, 2007;Kang et al, 2014), horses (Ball and Vo, 2001;Mantovani et al, 2002), chickens (Choi et al, 2012), and goats (Kim et al, 2006;Seifi-Jamadi et al, 2017).…”