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IntroductionSemen cryopreservation is one of the most effective and feasible method for preservation of genetic resources in avian species. This method enables storage of a very large number of samples in a relatively short time and is non-invasive for both donors and recipients. A cost comparison study pertaining to preservation of avian genetic resources had shown that cryopreservation of semen to be approximately ninety percent less expensive than the total cost of maintaining live birds [1]. The high vitellus content of megalecithal egg in avian species makes cryopreservation of oocyte and embryo difficult and the low efficacy of reconstitution and high cost associated with blastodermal or primordial germ cell preservation makes these approaches prohibitive [2]. Many freezing methods with slow and rapid freezing procedures, using variety of cryoprotective agents such as Glycerol, Dimethylacetamide (DMA), Dimethyl sulphoxide (DMSO), N-methylacetamide (MA), Sucrose, Trehalose and Polyvinyl pyrrolidone (PVP) and different types of sperm packaging methods (pellets in plastic/glass ampoules and French straws) have been explored to cryopreserve rooster sperm [3][4][5][6][7][8][9]. Gramapriya is a multi-coloured layer type chicken variety developed at ICAR-Directorate of Poultry Research, Hyderabad, for free range farming in rural and tribal areas and for rural backyard rearing.PD6 developed from multicoloured broiler population has been selected for shank length for six generations and is used as male line, and PD3 used for production of coloured germplasm for free range farming is used as female line to produce rural poultry cross "Gramapriya". The rural and tribal farmers of many states of India are being profited by this variety. Considering the importance of the PD6 (male line)

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To explore the effect of kisspeptin on in vitro maturation of buffalo oocytes under different hormonal combinations. For the present study, Kp@10 µg/ml was selected as optimum by assessing the proportion of Cumulus Cell Expansion (CCE) and extrusion of 1st polar body (PB) from the earlier study conducted. Media containing TCM 199 (supplemented with Gentamicin) and oocytes were used as control. Overall, oocytes were allowed to mature in 12 different maturation media viz., Control (T1), Kp at 10 µg/ml (T2), FSH alone (T3), FSH +Kp (T4), LH (T5), LH +Kp (T6), E2 (T7), E2+Kp (T8), FSH+LH+E2 (T9), FSH+LH+E2+Kp (T10), FSH+LH+E2+FCS+BSA (T11) and FSH+LH+E2+FCS+BSA+Kp (T12), incubated at 38.5°C and 5% CO2 under humidified atmosphere for 22 hours. The results showed significantly (P £ 0.5) higher percentages of CCE and PB in groups supplemented with Kp (T4, T6, T8, T10 and T12,) compared to control (T1) and other treatment groups supplemented with individual hormones either alone (T3, T5 and T7) or combination of hormones (T9) and IVM alone (T11). Therefore, it has been clearly demonstrated that addition of Kp (10 µg/ml) to FSH, LH, E2 supplemented media, in various combinations enhances buffalo oocyte maturation rates in vitro.