Production of pullulan by Aureobasidium pullulans from Asian palm kernel: A novel substrate

Sugumaran, K.R.; Gowthami, E.; Swathi, B.; Elakkiya, S.; Srivastava, Supriya; Ravikumar, R.; Gowdhaman, D.; Venkatachalam, P.

doi:10.1016/j.carbpol.2012.09.062

Cited by 75 publications

(17 citation statements)

References 45 publications

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“…The peaks appearing at 3400 cm −1 and 2930 cm −1 were attributed to OH stretching and CH stretching, respectively. Further characteristic signals obtained at 1368, 1155, and 1080 cm −1 were attributed to C–OH bending, C–O–C stretching, and C–O stretching, respectively 27 . Other features of the biopolymer were also found from the spectra, including O–C–O stretch (1656 cm −1 ), and C–O–C stretch (1155 cm −1 ), as reported previously 28 .…”

Section: Resultssupporting

confidence: 83%

Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose

Chang

et al. 2017

Brazilian Journal of Microbiology

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Pullulan is a natural exopolysaccharide with many useful characteristics. However, pullulan is more costly than other exopolysaccharides, which limits its effective application. The purpose of this study was to adopt a novel mixed-sugar strategy for maximizing pullulan production, mainly using potato starch hydrolysate as a low-cost substrate for liquid-state fermentation by Aureobasidium pullulans. Based on fermentation kinetics evaluation of pullulan production by A. pullulans 201253, the pullulan production rate of A. pullulans with mixtures of potato starch hydrolysate and sucrose (potato starch hydrolysate:sucrose = 80:20) was 0.212 h−1, which was significantly higher than those of potato starch hydrolysate alone (0.146 h−1) and mixtures of potato starch hydrolysate, glucose, and fructose (potato starch hydrolysate:glucose:fructose = 80:10:10, 0.166 h−1) with 100 g L−1 total carbon source. The results suggest that mixtures of potato starch hydrolysate and sucrose could promote pullulan synthesis and possibly that a small amount of sucrose stimulated the enzyme responsible for pullulan synthesis and promoted effective potato starch hydrolysate conversion effectively. Thus, mixed sugars in potato starch hydrolysate and sucrose fermentation might be a promising alternative for the economical production of pullulan.

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Section: Resultssupporting

confidence: 83%

Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose

Chang

et al. 2017

Brazilian Journal of Microbiology

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“…The absorption band in the FT-IR spectrum (Additional file 1 : Figure S1a) at 3394 cm −1 and 2928 cm −1 were characteristic of O-H stretching and C-H stretching, respectively. The characteristic signals appeared at 1637, 1420, 1152 and 1020 cm −1 were due to O-C-O stretching, C-O-H bending, C-O-C stretching and C-O stretching, respectively [ 25 ]. Furthermore, the signals arrived in the 1 H-NMR spectrum (Additional file 1 : Figure S1b) between 4 and 5.5 ppm infers proton carrying carbon atoms attached to an electronegative atom, signals at 1.0 ppm was due to 6-deoxy-D-altrose present in the pullulan [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

“…The characteristic signals appeared at 1637, 1420, 1152 and 1020 cm −1 were due to O-C-O stretching, C-O-H bending, C-O-C stretching and C-O stretching, respectively [ 25 ]. Furthermore, the signals arrived in the 1 H-NMR spectrum (Additional file 1 : Figure S1b) between 4 and 5.5 ppm infers proton carrying carbon atoms attached to an electronegative atom, signals at 1.0 ppm was due to 6-deoxy-D-altrose present in the pullulan [ 25 ]. 13 C-NMR for the purified exopolysaccharide was shown in Additional file 1 : Figure S1c, the anomeric carbon region shows three signals corresponding to α -(1 → 4) (101.8, 101.1 ppm) and α -(1 → 6) (99.2 ppm) linkages, splitting of C-6 (60.7, 61.3 ppm) and C-4 (80.4, 81.2 ppm) were due to C-1 of (1 → 4) linked glucose unit, the signal resonance at 67.4 ppm corresponds to C-6 of the 1,6-linked α -D-glucose [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…FT-IR was performed on a Nicolet 560 FT-IR spectrometer (Thermo, USA) and the sample was blended with KBr pellet. The 1 H and 13 C-NMR spectra were recorded using a Bruker AVANCE AV-400 spectrophotometer (Bruker Biospin Corp., Billerica, MA), 10 mg of the purified exopolysaccharide was dissolved in 0.5 mL DMSO-d 6 solvent and TMS was used as an internal standard for proton NMR [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

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Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

Xia¹,

Xu²,

Liu³

et al. 2017

BMC Biotechnol

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Backgroundpoly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-scale production. To overcome this problem, PMA production from Jerusalem artichoke tubers was successfully performed. Additionally, a valuable exopolysaccharide, pullulan, was co-produced with PMA by Aureobasidum pullulans HA-4D.ResultsThe Jerusalem artichoke medium for PMA and pullulan co-production contained only 100 g/L hydrolysate sugar, 30 g/L CaCO3 and 1 g/L NaNO3. Compared with the glucose medium, the Jerusalem artichoke medium resulted in a higher PMA concentration (114.4 g/L) and a lower pullulan concentration (14.3 g/L) in a 5 L bioreactor. Meanwhile, the activity of pyruvate carboxylase and malate dehydrogenas was significantly increased, while the activity of α-phosphoglucose mutase, UDP-glucose pyrophosphorylase and glucosyltransferase was not affected. To assay the economic-feasibility, large-scale production in a 1 t fermentor was performed, yielding 117.5 g/L PMA and 15.2 g/L pullulan.ConclusionsIn this study, an economical co-production system for PMA and pullulan from Jerusalem artichoke was developed. The medium for PMA and pullulan co-production was significantly simplified when Jerusalem artichoke tubers were used. With the simplified medium, PMA production was obviously stimulated, which would be associated with the improved activity of pyruvate carboxylase and malate dehydrogenas.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-017-0340-y) contains supplementary material, which is available to authorized users.

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“…To survive at 25 °C, it is likely that some basic nutrients have to be available. Some strains of A. pullulans have been reported to have higher temperature requirements, for example 30 °C was preferred for strains of A. pullulans isolates from India [43]. Y126 was isolated in the UK where the climate is colder, so this isolate may be less well adapted to high temperatures.…”

Section: Mortality Of Antagonists B91 and Y126mentioning

confidence: 99%

Identification and Characterisation of New Microbial Antagonists for Biocontrol of Monilinia laxa, the Causal Agent of Brown Rot on Stone Fruit

Rungjindamai

Jeffries

2013

Agronomy

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Abstract:Monilinia laxa is the causal agent of brown rot disease on stone fruits, and also causes blossom wilt and twig canker. The common practice used to manage this disease is through fungicide treatments. However the demand to reduce fungicide inputs has been increasing and there is a growing number of reports of M. laxa strains that are resistant to fungicides. There is an urgent need to search for an alternative strategy to control the disease. This study focused on the isolation and characterisation of biological control agents (BCAs) using indigenous isolates isolated from cherries and plums collected within the UK. A total of 192 isolates were screened against two strains of M. laxa in a series of in vitro dual culture tests. From this in vitro screen, 12 isolates were selected for a subsequent in vivo screen on detached fruits, which then narrowed these isolates down to two potential BCAs. These two strains were identified as Bacillus amyloliquefaciens/subtilis (isolate B91) and Aureobasidium pullulans (isolate Y126). The capability of these two potential BCAs to grow and survive at a range of temperatures likely to be experienced under field and storage conditions was studied in order to gain knowledge for product formulation and field application. Bacillus sp. B91 was shown to be a mesophilic bacterium that could grow at 10-25 °C but suffered significant mortality at 0 and 5 °C, while A. pullulans Y126 was both mesophilic and psychrotolerant as it grew between 0-25 °C with the optimum at 20 °C. When all nutrients were removed, Y126 was able to survive for several weeks in all test temperatures (0-25 °C) but showed significant OPEN ACCESSAgronomy 2013, 3 686 mortality at 25 °C. The capability of B91 to survive at 20 and 25 °C was higher than at low temperatures (0-15 °C). In addition, the modes of action of the potential BCAs were studied. B91 was shown to produce soluble and volatile organic compounds that inhibited M. laxa, while A. pullulans Y126 did not produce inhibitory compounds, but appeared to inhibit the pathogen via competition for nutrients. This study shows that microbial antagonists against M. laxa can be found from indigenous sources and that they are capable of preventing brown rot disease in controlled conditions, thus demonstrating a potential to be developed into commercial products.

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Production of pullulan by Aureobasidium pullulans from Asian palm kernel: A novel substrate

Cited by 75 publications

References 45 publications

Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose

Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose

Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

Identification and Characterisation of New Microbial Antagonists for Biocontrol of Monilinia laxa, the Causal Agent of Brown Rot on Stone Fruit

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