Effect of Kisspeptin on in vitro Maturation of Buffalo Oocytes

Rajesh, Kolipaka; Swathi, B.; Kumari, G. Aruna; Shanmugam, M.

doi:10.18805/ijar.b-3884

Cited by 3 publications

(3 citation statements)

References 21 publications

(25 reference statements)

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“…In cattle, the addition of KISS1 during IVC and IVF increased the proportion of blastocysts relative to gonadotropins [ 198 ]. Similar beneficial effects of KISS1 on IVF oocyte maturation and developmental competence have been reported in buffalo [ 199 ] and pigs [ 132 , 200 ]. The addition of KISS1 to cultured pig COCs improved oocyte maturation and increased the blastocyst formation rate [ 132 ].…”

Section: Application Of Kiss1 In Assisted Reproduction In Livestocksupporting

confidence: 76%

Peripheral action of kisspeptin at reproductive tissues—role in ovarian function and embryo implantation and relevance to assisted reproductive technology in livestock: a review

D’Occhio

Campanile

Baruselli

2020

Biology of Reproduction

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Kisspeptin (KISS1) is encoded by the KISS1 gene and was initially found to be a repressor of metastasis. Natural mutations in the KISS1 receptor gene (KISS1R) were subsequently shown be associated with idiopathic hypothalamic hypogonadism and impaired puberty. This led to interest in the role of KISS1 in reproduction. It was established that KISS1 had a fundamental role in the control of GnRH secretion. KISS1 neurons have receptors for leptin and estrogen receptor α (ERα) which places KISS1 at the gateway of metabolic (leptin) and gonadal (ERα) regulation of GnRH secretion. More recently, KISS1 has been shown to act at peripheral reproductive tissues. KISS1 and KISS1R genes are expressed in follicles (granulosa, theca, oocyte), trophoblast and uterus. KISS1 and KISS1R proteins are found in the same tissues. KISS1 appears to have autocrine and paracrine actions in follicle and oocyte maturation, trophoblast development, and implantation and placentation. In some studies, KISS1 was beneficial to in vitro oocyte maturation and blastocyst development. The next phase of KISS1 research will explore potential benefits on embryo survival and pregnancy. This will likely involve longer-term KISS1 treatments during proestrus, early embryo development, trophoblast attachment, and implantation and pregnancy. A deeper understanding of the direct action of KISS1 at reproductive tissues could help to achieve the next step change in embryo survival and improvement in the efficiency of assisted reproductive technology.

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Section: Application Of Kiss1 In Assisted Reproduction In Livestocksupporting

confidence: 76%

Peripheral action of kisspeptin at reproductive tissues—role in ovarian function and embryo implantation and relevance to assisted reproductive technology in livestock: a review

D’Occhio

Campanile

Baruselli

2020

Biology of Reproduction

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“…The Kp effect on buffalo oocyte maturation was first reported in India and standardized concentration with 10 µg/ml and achieved optimum oocyte maturation (73.21%), increasing concentration decreased the maturation (68.77%). In the case of first polar body extrusion also Kp was positively correlated (Rajesh et al, 2018) [29] . The Kp has autocrine action and local interaction with hormones and modulates the FSH, LH, and E2 receptors on oocytes.…”

Section: Buffalomentioning

confidence: 99%

Role of Kisspeptin on the reproductive system of livestock and pet animals

Rajendar¹,

Bera²,

Shah³

et al. 2023

Int. J. Vet. Sci. Anim. Husbandry

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The livestock species economically enhance the quality of life of farmers and industries and reproduction is the only way to progress in the next generation. Kisspeptin (Kp) is a neuropeptide that acts on the reproductive system and is associated with reproductive disorders in mammalians. The different forms of Kp are present but only kp-14 are more prominent to use due to their short length. The Kp is act with the orphan G-protein coupled receptor 54 (GPR54) ligands and activates the hypothalamic-pituitary-gonadal axis (HPG) to release GnRH and reproductive hormones. Even the Kp is a reproductive neuropeptide, it's limited to a few species to conduct the research and needs to perform more research on various species. The Kp may act powerful supplement in animal reproductive techniques and this review focuses on the livestock and pet animal reproductive system regulated by Kp.

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“…Supplementation of the IVM media with gonadotropins and estradiol has been found to be essential for acquisition of developmental capacity of oocytes in cattle (Brackett et al 1989). Supplementation of the IVM media (Rajesh et al 2020) with FCS (Totey et al 1993) or estrus cow serum (Madan et al 1994), follicular fluid (Das et al 1996) has also been found to be necessary for achieving high maturation rates for cattle and buffalo oocytes. Supplementing the culture media with growth factors stimulated embryo development beyond the 8-cell stage (Thibodeaux et al 1993;Yang et al 1993;Borah and Biswas, 2020).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Developmental Competence of Immature Oocytes Supplemented with Growth Factors in Culture Media

Umdor

Karunakaran

Mandal

et al. 2021

IJAR

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Background: In vitro embryo production is a valuable tool for understanding early mammalian development, therapeutic applications, excellent source for research in the field of developmental biology and production of valuable animals. The purpose of this study is to improve the production of in vitro cattle embryos using fibroblast and platelet derived growth factor as media supplement. Methods: Ovaries were collected from local abattoir in 0.9% saline (30-35°C) supplemented with antibiotics. Cumulus oocyte complexes were aspirated, washed 5-6 times and placed in maturation media supplemented with growth factors and cultured in 5% CO2 incubator at 38.5°C with maximum humidity. After 24 h oocytes were co-incubated with in vitro capacitated sperms for fertilization for 15-18 h and then presumptive zygotes were cultured for embryo development. Cleavage was observed after 40-42 h and embryos were co-cultured with oviductal cells for 7-9 days. Result: The highest cleavage and blastocyst formation rates were 55.93 ± 4.75, 57.06 ± 4.78, 51.24 ± 4.12 and 3.26 ±1.53, 2.42 ± 1.02, 2.70 ± 1.17 in FGF (1ng ml-1), PDGF (10 ng ml-1) and in combination of FGF and PDGF (1ng ml-1 each) respectively. It can be concluded that PDGF (10 ng ml-1) enhanced cleavage rate and FGF (1ng ml-1) enhanced blastocyst formation rate.

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Effect of Kisspeptin on in vitro Maturation of Buffalo Oocytes

Cited by 3 publications

References 21 publications

Peripheral action of kisspeptin at reproductive tissues—role in ovarian function and embryo implantation and relevance to assisted reproductive technology in livestock: a review

Peripheral action of kisspeptin at reproductive tissues—role in ovarian function and embryo implantation and relevance to assisted reproductive technology in livestock: a review

Role of Kisspeptin on the reproductive system of livestock and pet animals

Enhancement of Developmental Competence of Immature Oocytes Supplemented with Growth Factors in Culture Media

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