Effects of NaCl Stress on Callus Proliferation and Plant Regeneration from Mature Embryos of Bread Wheat (&lt;i&gt;Triticum aestivum&lt;/i&gt; L.) Cultivars Mahon Demias and Hidhab

Benderradji, Laid; Bouzerzou, H; Djekoun, Abdelhamid; Benmahammed, Amar

doi:10.3329/ptcb.v17i1.1117

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…Nevertheless, the studies about behavior under saline conditions in stylosanthes species referred only to germination (Gonela et al 2004, Silva et al 2014) and plant growth (Lovato and Martins 1997;Gonzá-lez et al 2000;Fuentes et al 2010). In this regard, programs of in vitro selection for tolerance to abiotic stresses could be a useful tool; however, this strategy is dependent on the development of efficient and reliable callus induction and plant regeneration systems (Benderradji et al 2007). Following this orientation, callus cultures are commonly used as an in vitro technique for biochemical and physiological studies of the response to salt and water stress at cellular level (Gandonou et al 2006;Liu et al 2006;Kaviani 2008).…”

Section: Introductionmentioning

confidence: 99%

Monitoring of cell wall modifications during callogenesis in Stylosanthes guianensis (Fabaceae) under salt stress conditions

et al. 2015

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Callogenesis was induced from Stylosanthes guianensis (Aubl.) Sw. cv. CIAT-184 hypocotyl explants cultured in the presence of different concentrations of NaCl. Whereas calluses formed at 50 mM NaCl did not show significant changes, concentrations in the range of 100-250 mM NaCl influenced callus formation and survival. The concentrations which reduced the callus formation and survival by 50 % with respect to the controls (I 50 ) were 190 and 225 mM NaCl, respectively. Callus growth was also affected being 160 mM NaCl the concentration that reduced the fresh weight gain in 50 %, in comparison to the control. The cell walls of calluses formed at higher NaCl concentrations showed a lower cellulose content, which was accompanied by an increase in protein, phenolics, and esters, as revealed by Fourier transform infrared spectroscopy. These overall cell wall modifications were accompanied by the formation of cell wall appositions enriched with homogalacturonan and rhamnogalacturonan components, and also with an increase of arabinogalactan proteins in cell walls. These results evidence the ability of cells to adjust the cell wall composition under salt stress conditions, as part of the strategy to sustain their growth in such environment.

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Section: Introductionmentioning

confidence: 99%

Monitoring of cell wall modifications during callogenesis in Stylosanthes guianensis (Fabaceae) under salt stress conditions

et al. 2015

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“…In vitro technology, especially plant tissue culture offers many unconventional techniques for crop improvement (Dennis Thomas and Sreejesh, 2004). In vitro culture of plant cells and tissue has attracted considerable interest over the recent years because it provides the means to study the physiological and genetic processes of plants in addition of offering the potential to assist in breeding improved cultivars by increasing the genetic variability (Benderradji et al, 2007). According to Larkin and Scowcroft (1981) it is now generally accepted that plant cell culture itself generates genetic variability increasing cell mutation frequency.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Selection of Foeniculum vulgare for Salt Tolerance

KHORAMI

Safarnejad

2011

Not Sci Biol

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In vitro selection of Foeniculum vulgare for salt tolerance was undertaken by the use of somaclonal variation. In this idea, explants of root, hypocotyl and cotyledon of sterilized seedling were transferred to callus and regeneration media with concentrations of 0, 50, 100 and 150 mM of NaCl. After 4 weeks, calli induction, regeneration frequency and calli fresh and dry weights, in both control and stress conditions, were measured. The results showed that salinity caused a significant decrease in the callus induction and shoot regeneration of fennel. However, in the presence of 100 and 150 mM NaCl, the highest frequency of callus induction in hypocotyl and cotyledon explants was recorded on the media supplemented with 1 mg l-1 IAA (Indol-3-Acetic Acid) plus 1 mg l-1 2,4-D (2, 4- dichlorophenoxyacetic acid) and 2 mg l-1 kinetin. Among different growth regulator treatments, the combination of 2 mg l-1 NAA (Naphtaleneacetic acid) and 0.5 mg l-1 kinetin was found to be the most effective for shoot regeneration under stress condition. The highest dose of NaCl (150 mM) inhibited callus induction and shoot regeneration compared to control with 41% and 96% respectively. The calli fresh and dry weights of all explants were decreased with the increas of NaCl concentration. The highest and the lowest of dry and fresh weight of calli were observed in 0 and 150 mM respectively.

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“…Similarly, NaCl (0, 250 and 500 mM) were used to create salinity effect on 10-d-old seedlings of wheat (Mutlu et al 2009). Two wheat cultivars, Mahon Demias and Hidhab exhibited appreciable callus induction but differed significantly in the capacity of calli proliferation and regeneration under salinity stress (Benderradji et al 2007). In vitro studies on salt tolerance were also carried out with two indica rice cultivars, CSR27 (salt tolerant) and HBC19 (salt sensitive).…”

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confidence: 99%

Introduction of OsglyII gene into Oryza sativa for increasing salinity tolerance

Wani¹,

Gosal²

2011

Biologia plant.

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Mature seed-derived embryogenic calli of indica rice (Oryza sativa L. cv. PAU201) were induced on semisolid Murashige and Skoog medium supplemented with 2.5 mg dm -3 2,4-dichlorophenoxyacetic acid + 0.5 mg dm -3 kinetin + 560 mg dm -3 proline + 30 g dm -3 sucrose + 8 g dm -3 agar. Using OsglyII gene, out of 3180 calli bombarded, 32 plants were regenerated on medium containing hygromycin (30 mg dm -3 ). Histochemical GUS assay of the hygromycin selected calli revealed GUS expression in 50 % calli. Among the regenerants, 46.87 % were GUS positive. PCR analysis confirmed the presence of the transgene of 1 kb in 60 % of independent plants. Further, these plants have been grown to maturity in glasshouse. In vitro screening for salt tolerance showed increase in fresh mass of OsglyII putative transgenic calli (185.4 mg) as compared to control calli (84.2 mg) on 90 mM NaCl after 15 d. When exposed to 150 mM NaCl, OsglyII putative transgenic plantlets showed normal growth while the non-transgenic control plantlets turned yellow and finally did not survive.

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Effects of NaCl Stress on Callus Proliferation and Plant Regeneration from Mature Embryos of Bread Wheat (<i>Triticum aestivum</i> L.) Cultivars Mahon Demias and Hidhab

Cited by 5 publications

References 22 publications

Monitoring of cell wall modifications during callogenesis in Stylosanthes guianensis (Fabaceae) under salt stress conditions

Monitoring of cell wall modifications during callogenesis in Stylosanthes guianensis (Fabaceae) under salt stress conditions

In Vitro Selection of Foeniculum vulgare for Salt Tolerance

Introduction of OsglyII gene into Oryza sativa for increasing salinity tolerance

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