Effects of nanoplastic exposure on the immunity and metabolism of red crayfish (Cherax quadricarinatus) based on high-throughput sequencing

Cheng, Huitao; Dai, Yuantang; Ruan, Xinhe; Duan, Xuzhuo; Zhang, Chunli; Li, Lihua; Huang, Fengqi; Shan, Jinhong; Liang, Kaishan; Jia, Xianze; Wang, Qing; Zhao, Hongbin

doi:10.1016/j.ecoenv.2022.114114

Cited by 15 publications

(4 citation statements)

References 59 publications

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“…Transcriptome analysis is widely used in the study of molecular level in biology along with the development of sequencing technologies. In recent years, transcriptome analysis has gradually been applied in the toxicology research of NPs on aquatic organisms, such as B. plicatilis , D. pulex , Isognomon alatus , Mytilus coruscus , Cherax quadricarinatus , C. robusta , D. rerio , Oreochromis mossambicus , I. punetaus ( Liu et al, 2021a ; Pang et al, 2021 ; Arini et al, 2022 ; Yu et al, 2022b ; Cheng et al, 2022 ; Jiang et al, 2022 ; Shin and Jeong, 2022 ; Eliso et al, 2023 ; Qi et al, 2023 ). To research the effect of NPs on the molecular level of S. esculenta larvae, we applied RNA-Seq technology in this research.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of oxidative stress mechanisms induced by acute nanoplastic exposure in Sepia esculenta larvae

Liu

Yang

2023

Front. Physiol.

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Nanoplastics (NPs), as a new type of pollutant with a size small than 1 μm, are ubiquitous and harmful to organisms. There has been an increasing amount of research concerning the effects of NPs on organisms over recent years, especially on aquatic animals. However, there is a limited study on the impact of NPs on mollusk cephalopods. In this research, Sepia esculenta, belonging to Cephalopoda, Coleoidea, Sepioidea, was selected to explore the effects caused by NPs exposure. The S. esculenta larvae were exposed to polystyrene NPs (PS-NPs) with diameter 50 nm (100 mg/L) for 4 h. The detection of oxidative stress biomarkers displayed an obvious increase in SOD (superoxide dismutase) activity and MDA (malondialdehyde) level. Then, RNA-Seq was performed to explore the oxidative stress response at mRNA level. The transcriptome analysis demonstrated that the expression of 2,570 genes was affected by PS-NPs. Besides, the signaling pathways of ribosome, ribosome biogenesis in eukaryotes, proteasome, and MAPK were enriched. This study not only provides novel references for understanding the mechanisms of oxidative stress response induced by NPs, but also reminds us to follow with interest the influence of acute exposure to NPs.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of oxidative stress mechanisms induced by acute nanoplastic exposure in Sepia esculenta larvae

Liu

Yang

2023

Front. Physiol.

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“…Further, significant differences were found in the composition of the gut microbiome after infection with a new-emerging viral pathogen, namely the Decapod iridescent virus 1 (DIV1) [ 111 ]. Interestingly, the effects of nanoplastics on C. quadricarinatus led to significant changes in gut microbiota, including a decrease in abundance of Bacteroidetes, Actinobacteria, and Firmicutes [ 115 ]. Other necessary studied parameters in assessing their effect on the intestinal microbiota of crayfish are supplementation with trace elements (manganese, silica, and phosphorus and two different biological filters, i.e., Gravel, Bio-Ball).…”

Section: Main Factors Affecting Crayfish Microbiota Abundances and Co...mentioning

confidence: 99%

“…Data for Figures A and B obtained from Ref. [ 115 ]. C. cainii and C. quadricarinatus photos retrieved from Refs.…”

Section: Figurementioning

confidence: 99%

Probiotics, Prebiotics, and Synbiotics Utilization in Crayfish Aquaculture and Factors Affecting Gut Microbiota

et al. 2023

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Aquaculture is affected by numerous factors that may cause various health threats that have to be controlled by the most environmentally friendly approaches. In this context, prebiotics, probiotics, and synbiotics are frequently incorporated into organisms’ feeding rations to ameliorate the health status of the host’s intestine, enhancing its functionality and physiological performance, and to confront increasing antimicrobial resistance. The first step in this direction is the understanding of the complex microbiome system of the organism in order to administer the optimal supplement, in the best concentration, and in the correct way. In the present review, pre-, pro-, and synbiotics as aquaculture additives, together with the factors affecting gut microbiome in crayfish, are discussed, combined with their future prospective outcomes. Probiotics constitute non-pathogenic bacteria, mainly focused on organisms’ energy production and efficient immune response; prebiotics constitute fiber indigestible by the host organism, which promote the preferred gastrointestinal tract microorganisms’ growth and activity towards the optimum balance between the gastrointestinal and immune system’s microbiota; whereas synbiotics constitute their combination as a blend. Among pro-, pre-, and synbiotics’ multiple benefits are boosted immunity, increased resistance towards pathogens, and overall welfare promotion. Furthermore, we reviewed the intestinal microbiota abundance and composition, which are found to be influenced by a plethora of factors, including the organism’s developmental stage, infection by pathogens, diet, environmental conditions, culture methods, and exposure to toxins. Intestinal microbial communities in crayfish exhibit high plasticity, with infections leading to reduced diversity and abundance. The addition of synbiotic supplementation seems to provide better results than probiotics and prebiotics separately; however, there are still conflicting results regarding the optimal concentration.

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“…Electronic copy available at: https://ssrn.com/abstract=4455299 P r e p r i n t n o t p e e r r e v i e w e d Pont et al, 2016). Antioxidant and xenobiotic responses have been described upon exposure to PS NP, such as the up-regulation of glutathione S-transferase, in aquatic invertebrates like Daphnia pulex (Liu et al, 2021), and the crayfish Cherax quadricarinatus (Cheng et al, 2022). Interestingly, translation and antioxidant transcript expression has been altered at 5 and 50 µg/ml respectively, similar to what was reported for the swamp crayfish, Procambarus clarki, upon PS NP orally administered (Capanni et al, 2021).…”

Section: " the Expression Of Two Genes (For Transcripts Trinity_dn246...mentioning

confidence: 99%

Transcriptomic Responses of Antarctic Clam Laternula Elliptica to Nanoparticles, at Single and Combined Exposures: Revealing Ecologically Relevant Biomarkers

Rondon,

Valdés,

Cosseau

et al. 2023

Preprint

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Effects of nanoplastic exposure on the immunity and metabolism of red crayfish (Cherax quadricarinatus) based on high-throughput sequencing

Cited by 15 publications

References 59 publications

Transcriptomic analysis of oxidative stress mechanisms induced by acute nanoplastic exposure in Sepia esculenta larvae

Transcriptomic analysis of oxidative stress mechanisms induced by acute nanoplastic exposure in Sepia esculenta larvae

Probiotics, Prebiotics, and Synbiotics Utilization in Crayfish Aquaculture and Factors Affecting Gut Microbiota

Transcriptomic Responses of Antarctic Clam Laternula Elliptica to Nanoparticles, at Single and Combined Exposures: Revealing Ecologically Relevant Biomarkers

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