Effects of natural antimicrobials on bacterial cell hydrophobicity, adhesion, and zeta potential / Vpliv naravnih protimikrobnih snovi na bakterijsko hidrofobnost, adhezijo in zeta potencial

Kurinčič, Marija; Jeršek, Barbara; Klančnik, Anja; Možina, Sonja Smole; Fink, Rok; Dražić, Goran; Raspor, Peter; Bohinc, Klemen

doi:10.1515/aiht-2016-67-2720

Cited by 38 publications

(11 citation statements)

References 31 publications

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“…This problem was observed and discussed in detail in the study of Rysanek et al [38]. However, the biofilm formation can be overcome by proper nanomaterial functionalization, preventing bacterial cell growth [37,55,61]. PAs functionalized with AgNO3 and CHX were also tested.…”

Section: Influence Of Pa Morphology and Functionalization On Their Rementioning

confidence: 99%

Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for Staphylococcus Aureus

et al. 2021

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Although nanomaterials are used in many fields, little is known about the fundamental interactions between nanomaterials and microorganisms. To test antimicrobial properties and retention ability, 13 electrospun polyamide (PA) nanomaterials with different morphology and functionalization with various concentrations of AgNO3 and chlorhexidine (CHX) were analyzed. Staphylococcus aureus CCM 4516 was used to verify the designed nanomaterials’ inhibition and permeability assays. All functionalized PAs suppressed bacterial growth, and the most effective antimicrobial nanomaterial was evaluated to be PA 12% with 4.0 wt% CHX (inhibition zones: 2.9 ± 0.2 mm; log10 suppression: 8.9 ± 0.0; inhibitory rate: 100.0%). Furthermore, the long-term stability of all functionalized PAs was tested. These nanomaterials can be stored at least nine months after their preparation without losing their antibacterial effect. A filtration apparatus was constructed for testing the retention of PAs. All of the PAs effectively retained the filtered bacteria with log10 removal of 3.3–6.8 and a retention rate of 96.7–100.0%. Surface density significantly influenced the retention efficiency of PAs (p ≤ 0.01), while the effect of fiber diameter was not confirmed (p ≥ 0.05). Due to their stability, retention, and antimicrobial properties, they can serve as a model for medical or filtration applications.

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Section: Influence Of Pa Morphology and Functionalization On Their Rementioning

confidence: 99%

Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for Staphylococcus Aureus

et al. 2021

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“…As a negative control, we used 200 µL of sterile TSB added in eight wells of each microtiter plate. The crystal violet assay has already been described [35,36]. After 24 h incubation at 30 • C, the supernatant was removed from each well and the plate was washed three times with 200 µL of distilled water.…”

Section: Crystal Violet Assay For Determination Of Biofilm Formation In Tsbmentioning

confidence: 99%

Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment

et al. 2020

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The study monitored the effect of tetracycline on bacterial biofilm formation and compared biofilm formation by resistant bacterial strains in different phases of the wastewater treatment process in wastewater treatment plant (WWTP). The crystal violet staining method was used to evaluate the biofilm formation. Biofilm-related bacterial properties were characterized by hydrophobicity, autoaggregation and motility tests. The relative abundance of tetracycline resistance genes (tetW, tetM, tetO, tetA and tetB) in wastewaters were subsequently quantified using qPCR. The results show that the isolates from the nitrification tank produce biofilm with up to 10 times greater intensity relative to the isolates from the sedimentation tank. In isolates of Aeromonas sp. from the nitrification tank, increased biofilm production in the occurrence of tetracycline from a concentration of 0.03125 µg/mL was observed. The tetW gene showed the highest relative abundance out of all the tested genes. From the sampling points, its abundance was the highest in the sedimentation tank of the WWTP. Based on these results, it can be assumed that resistant bacteria are able to form a biofilm and sub-inhibitory tetracycline concentrations induce biofilm formation. WWTPs thus represent a reservoir of antibiotic resistance genes and contribute to the spread of resistance in the natural environment.

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“…Antimicrobial peptides (AMPs) are an attractive solution as therapeutic agents, for their capability to kill a broad range of bacteria (including multidrug-resistant strains), fungi, and viruses and reducing the development of drug resistance [3–6]. Natural antimicrobial peptides (i.e., >750 compounds) are considered alternative agents with new active mechanisms of action and a wide diversity of active principles [7]. Bee venom (BV) is a complex mixture of peptides, enzymes, and biogenic amines possessing several pharmaceutical properties, whose main peptidic components are melittin and apamin.…”

Section: Introductionmentioning

confidence: 99%

Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection

Galdiero

Siciliano

Gesuele

et al. 2019

International Journal of Microbiology

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The emerging concern about the increase of antibiotic resistance and associated biofilm has encouraged scientists to look for alternative antibiotics such as antimicrobial peptides (AMPs). This study evaluated the ability of melittin to act as an antibacterial biofilm inhibitor and biofilm remover considering isolates from dairy industry. Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs), minimum biofilm inhibitory concentrations (MBICs), and biofilm removal activities were studied in polymicrobial biofilms produced from isolates. MIC and MBC were set at 1–3 µg/mL and 25–50 µg/mL for Gram-positive and Gram-negative bacteria, respectively. Results demonstrated a good MBIC reaching 85% inhibition ability and a good activity and better penetration in deeper layers against the mixed preformed biofilm, thereby increasing its activity against all isolates also at the lowest tested concentrations. Melittin showed interesting characteristics suggesting its potential to act as an antimicrobial agent for polymicrobial biofilm from dairy industry even in environmental isolates.

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Effects of natural antimicrobials on bacterial cell hydrophobicity, adhesion, and zeta potential / Vpliv naravnih protimikrobnih snovi na bakterijsko hidrofobnost, adhezijo in zeta potencial

Cited by 38 publications

References 31 publications

Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for Staphylococcus Aureus

Benefits of Polyamide Nanofibrous Materials: Antibacterial Activity and Retention Ability for Staphylococcus Aureus

Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment

Melittin Inhibition and Eradication Activity for Resistant Polymicrobial Biofilm Isolated from a Dairy Industry after Disinfection

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