Effects of neurotrophin receptor‐mediated MAGE homology on proliferation and odontoblastic differentiation of mouse dental pulp cells

Qi, Shengcai; Wu, Qi; Ma, Jie; Li, J.; Chen, F.; Xu, Yuanzhi; Pan, Qiuhui; Wang, R.

doi:10.1111/cpr.12171

Cited by 9 publications

(11 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our experiments consistently demonstrated that high GLU, particularly at the concentrations of 25 and 50 mM, markedly suppressed human DPC proliferation and promoted cell apoptosis. ALP is a marker of odontoblastic differentiation and its activity is enhanced during odontoblastic induction (33,34). We found that high GLU inhibited ALP activity and mineralization in DPCs, suggesting that high GLU inhibited the proliferation and differentiation and induced the apoptosis of DPCs.…”

Section: Discussionmentioning

confidence: 66%

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Lü

Sun

Liu

2017

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Insulin-like growth factor-1 (IGF-1) promotes human dental pulp stem cell proliferation and osteogenic differentiation. However, the effects of IGF-1 on the proliferation, apoptosis and odontoblastic differentiation (mineralization) of dental pulp cells (DPCs) under high glucose (GLU) conditions remain unclear. In this study, isolated primary human DPCs were treated with various concentrations of high GLU. Cell proliferation and apoptosis were determined by Cell Counting Kit-8 and Annexin V-FITC/PI assays, respectively. The cells were cultured in odontoblastic induction medium containing various concentrations of high GLU. Odontoblastic differentiation was determined by alkaline phosphatase (ALP) activity assay. Mineralization formation was evaluated by von Kossa staining. The expression levels of IGF family members were measured by western blot analysis and RT-qPCR during proliferation and differentiation. The cells were then exposed to 25 mM GLU and various concentrations of IGF-1. Cell proliferation, apoptosis, ALP activity, mineralization formation and the levels of mineralization-related proteins were then evaluated. Our results revealed that high GLU significantly inhibited cell proliferation and promoted cell apoptosis. GLU (25 and 50 mM) markedly reduced ALP activity and mineralization on days 7 and 14 after differentiation. The levels of IGF family members were markedly decreased by high GLU during proliferation and differentiation. However, IGF-1 significantly reversed the effects of high GLU on cell proliferation and apoptosis. Additionally, IGF-1 markedly restored the reduction of ALP activity and mineralization induced by high GLU. Our findings thus indicate that IGF-1 attenuates the high GLU-induced inhibition of DPC proliferation, differentiation and mineralization.

show abstract

Section: Discussionmentioning

confidence: 66%

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Lü

Sun

Liu

2017

International Journal of Molecular Medicine

View full text Add to dashboard Cite

show abstract

“…indicated that Mage‐D1‐deficient osteoclasts exhibited a higher expression of canonical NF‐kappaB and resulted in increased osteoclastogenesis but not osteoblastogenesis. Reports indicated that Mage‐D1 showed a negative effect on differential mineralization in differentiated cell lines similar to calvarial osteoblasts and dental pulp cells . Knockdown of Mage‐D1 significantly down‐regulated the potential of differential mineralization (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Reports indicated that Mage-D1 showed a negative effect on differential mineralization in differentiated cell lines similar to calvarial osteoblasts 26 and dental pulp cells. 51 Knockdown of Mage-D1 significantly down-regulated the potential of differential mineralization (Fig. 7a,b,d) in EMSCs, suggesting that the "connection" of p75NTR and Mage-D1 with mineralizationrelated genes was attenuated (Fig.…”

Section: Mage-d1 and Ngf Play Important Roles Of Mineralized Differmentioning

confidence: 96%

See 1 more Smart Citation

p75 neurotrophin receptor regulates differential mineralization of rat ectomesenchymal stem cells

Yang

Wang

et al. 2016

Cell Proliferation

View full text Add to dashboard Cite

show abstract

“…Leukaemia inhibitory factor promotes the proliferation of DPCs, and inhibits the odontoblastic differentiation via the Jak2‐Stat3 signalling pathway. NRAGE regulates the odontoblastic differentiation of hDPCs through NF‐kB signalling pathway . The ERK signalling pathway plays important roles in odontogenic differentiation of hDPSCs and repair after dental pulp injury.…”

Section: Discussionmentioning

confidence: 99%

The effect of delta‐like 1 homologue on the proliferation and odontoblastic differentiation in human dental pulp stem cells

Yan

Yang

et al. 2017

Cell Proliferation

View full text Add to dashboard Cite

show abstract

Effects of neurotrophin receptor‐mediated MAGE homology on proliferation and odontoblastic differentiation of mouse dental pulp cells

Abstract: NRAGE is a potent regulator of proliferation and odontoblastic differentiation of mDPCs, which might be via the NF-κB signalling pathway.

Cited by 9 publications

References 50 publications

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

p75 neurotrophin receptor regulates differential mineralization of rat ectomesenchymal stem cells

The effect of delta‐like 1 homologue on the proliferation and odontoblastic differentiation in human dental pulp stem cells

Contact Info

Product

Resources

About