Effects of nitrogen supplementation on yeast (Candida utilis) biomass production by using pineapple (Ananas comosus) waste extracted medium

Abstract

Pineapple waste medium was used to cultivate yeast, Candida utilis. It served as the sole carbon and energy source for the yeast growth. However, pineapple waste media contain very little nitrogen (0.003-0.015% w/v). Various nitrogen sources were incorporate and their effects on biomass, yield and productivity were studied. Significant (p<0.05) increment on biomass production was observed when nitrogen supplement (commercial yeast extract, peptone, ammonium dihydrogen phosphate, ammonium sulphate and potassium nitrate) was added into fermentation medium. Commercial yeast extract, Maxarome ® which increased 55.2% of biomass production at 0.09% (w/v) nitrogen content, is the most suitable among the selected organic source. On the other hand, ammonium dihydrogen phosphate at 0.09% (w/v) nitrogen content is comparable inorganic source which enhanced 53.7% of production. Total nitrogen content of each treatment at 0.05% (w/v) showed that nitrogen supplied was not fully utilized as substrate limitation in the fermentation medium.

Production ofCandidaBiomass from Hydrolysed Agricultural Biowaste

“…cell density of 2.73 and 2.76 for wheat bran and rice husk, respectively is reached at the end of the cultivation, while for the oats hydrolysate -it is 2.48. Rosma et al investigated similar processes of continuous adaptation of the cells and slow accumulation of biomass in the initial period of the cultivation of C. utilis in citrus waste (15). it is seen that with the two kinds of yeasts at the end of the process, a cultural liquid with high cell density is obtained.…”

“…Candida sp. is one of the most widely used organisms in the ScP production (1,2,15,16,20,21). over the years efforts have been made to use agricultural residues for production of microbial biomass.…”

“…over the years efforts have been made to use agricultural residues for production of microbial biomass. there have been several studies about production of microbial biomass from industrial and agricultural wastes such as yeast biomass from rice straw, rice bran (3,17,19) hydrolysate salad oil manufacturing wastewater (20), pineapple cannery effluent (15), cabbage (5), ram horn hydrolysate (12).…”

Bioprocess Development for High Cell Mass Production of the Probiotic Yeast-Kluyveromyces lactis

“…Nevertheless, there are uncertainties estimating the optimal concentration of yeast extract required to allow maximal cell growth. Rosma and Cheong [30] suggested that the initial yeast extract concentration ranges from 0 to 10 g.L -1 . The purpose of this study is to investigate the effect of different concentrations of yeast extract on cell biomass of K. lactis.…”

Avicelase Enzyme from Sawdust: Isolation, Production and Optimization

“…Type of substrate, enzyme assay technique or reagent used may also contributed to the contradict results. According to Rosma and Cheong [11] have found that for the fermentation medium with 0.01% (w/v) nitrogen content, potassium nitrate, KNO 3 showed the highest yield among the other inorganic nitrogen sources which are NH 4 H 2 PO 4 and (NH 4 ) 2 SO 4 . According to Kubisi and others [12], potassium nitrate is a naturally occurring mineral source of nitrogen, but only several yeasts are able to assimilate nitrate as a nitrogen source.…”