Effects of Norepinephrine and Norepinephrine Agonists and Antagonists on the Melanophores of the Fiddler Crab Uca Pugilator

Fingerman, Milton; Hanumante, M. M.; Fingerman, Sue W.; Reinschmidt, Dana C.

doi:10.2307/1548202

Cited by 39 publications

(4 citation statements)

References 34 publications

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“…Indeed, Fluoxetine has been used previously to test the mechanistic role of neurotransmitters such as serotonin. Injection by fluoxetine into the fiddler crab ( Uca pugilator ) results in a release of PDH and a darkening on the chromatophores (Fingerman et al 1981 ; Hanumante and Fingerman 1981 , 1983 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of the antidepressant fluoxetine on pigment dispersion in chromatophores of the common sand shrimp, Crangon crangon: repeated experiments paint an inconclusive picture

Ford

Feuerhelm

2020

Ecotoxicology

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The effects of antidepressants in the environment are starting to generate considerable interest due to the fact that neurotransmitters influence a range of biological processes. Crypsis is an important behavioural and physiological response in many crustaceans modulated by monoamine and pigment dispersing/concentrating hormones. This study aimed to develop a test methodology and investigate the effects of the selective serotonin reuptake inhibitor (SSRI), fluoxetine, on a chromatophore index and overall carapace ‘darkness’ in the common sand shrimp Crangon crangon. Adult shrimp were exposed for either 1 h, 1 day or 1 week across a range of nominal fluoxetine concentrations (10 ng/L, 100 ng/L and 1000 ng/L) and the chromatophore index or carapace percentage ‘darkness’ was recorded following 30 min on white and black substrates. These experiments were repeated three times using different specimens. Animals became significantly darker (~20%) on darker background and lighter on light backgrounds as one might expect. However, time periods over which the animals were recorded had a significant impact on the colouration suggesting habituation to laboratory conditions. Fluoxetine exposure came up as a significant factor in two of the three trials for the chromatophore index but the results was inconsistent between trials. There was a high degree of correlation between the chromatophore index and the percentage darkness analyses however, there was no significant effects for fluoxetine exposure with the percentage darkness data. We conclude that the effects on antidepressants on colour change remain inconclusive from these experiments and we discuss potential areas to improve the repeatability of the experiments.

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Section: Introductionmentioning

confidence: 99%

Effects of the antidepressant fluoxetine on pigment dispersion in chromatophores of the common sand shrimp, Crangon crangon: repeated experiments paint an inconclusive picture

Ford

Feuerhelm

2020

Ecotoxicology

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“…Staub and Fingerman (1984) provided evidence to support the hypothesis that the inhibition of black pigment dispersion produced by exposure to naphthalene was due to inhibition of norepinephrine release. Perhaps cadmium similarly inhibits norepinephrine release, which because norepinephrine triggers release of both the black pigment-dispersing hormone and the light-adapting hormone (Fingerman et al 1981;Kulkarni and Fingerman 1986), would result in reduced black pigmentdispersing hormone and light-adapting hormone release and hence less dispersion of the pigment in the black chromatophores and less light adaptation of the distal pigment. Norepinephrine was able to produce light-adapting responses in the cadmium exposed crabs (Table 3) which offset at least in part the inhibitory effect of cadmium on the distal pigment.…”

Section: Resultsmentioning

confidence: 99%

“…Exposure of this crab to naphthalene (Staub and Fingerman 1984) or cadmium (Reddy and Fingerman 1995) results in decreased ability to disperse the pigment in their black chromatophores, the exposed crabs becoming paler than the unexposed crabs. Norepinephrine triggers release of both the black pigment-dispersing hormone (Fingerman et al 1981) and the light-adapting hormone (Kulkarni and Fingerman 1986). In view of the facts that (a) these hormones which regulate the black chromatophores and distal pigment are synthesized in and released from the eyestalk neuroendocrine complex (Kulkarni and Fingerman 1991), (b) the black pigment-dispersing hormone and the light-adapting hormone may actually be the same hormone, having two different activities (Riehm and Rao 1982) and (c) release of both the black pigment-dispersing hormone and the light-adapting hormone is triggered by norepinephrine, the present investigation was carried out to determine the effect of cadmium on distal pigment migration in Uca pugilator.…”

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confidence: 99%

Effect of Cadmium Chloride on the Distal Retinal Pigment Cells of the Fiddler Crab, Uca pugilator

Obih

et al. 1997

Bulletin of Environmental Contamination and Toxicology

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“…6-OHDA inhibits the rate of melanin dispersion in fiddler crabs, Uca pugilator, having maximally aggregated melanin initially and transferred from a white background to a black one (Hanumante and Fingerman, 1982b). The fact that of the possible neurotransmitters tested at 8 ^g/dose (including octopamine, NE, dopamine, and epinephrine) by Fingerman et al (1981) only NE induced melanin dispersion in the fiddler crab strongly suggests that the inhibition of melanin dispersion produced by 6-OHDA was due to its destructive action on noradrenergic terminals. This, together with the failure of HA to further reduce significantly the rate of centrifugal melanin migration in crabs pretreated with 6-OHDA (Fig.…”

Section: Discussionmentioning

confidence: 99%

INHIBITORY EFFECT OF HISTAMINE ON THE RELEASE OF MELANIN-DISPERSING HORMONE IN THE FIDDLER CRAB,UCA PUGILATOR

Hanumante¹,

Fingerman²

1982

The Biological Bulletin

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Histamine (HA), a stimulator of H, and H 2 receptors, produced dose-dependent inhibition of the melanin dispersion which normally occurs when fiddler crabs, Uca pugilator, are transferred from a white to a black background. The HA precursor L-histidine, and 4-methyl histamine (4-MeHA), an H 2 receptor agonist, also inhibited melanin dispersion. 2-Methyl histamine (2-MeHA), an H, receptor agonist, enhanced melanin dispersion. The inhibitory effects of HA and 4-MeHA were abolished by the H 2 receptor blocker metiamide but not by blockers of either H, receptors or alpha, adrenoceptors. Melanin-dispersing hormone (MDH) release is accomplished mainly by stimulation of alpha, adrenoceptors with norepinephrine appearing to be the neurotransmitter involved. The H, receptor blockers pyrilamine and SA-97 antagonized 2-MeHA. HA-induced inhibition of melanin dispersion was potentiated by the noradrenergic neuron blocker bretylium and the alpha 2 adrenoceptor agonist B-HT 933. HA did not significantly affect melanin dispersion in crabs pretreated with 6-hydroxydopamine which destroys catecholaminergic neuroterminals. None of these drugs affected the melanophores directly. On the basis of these and previously obtained results it is suggested that H, and H 2 receptors are present on norepinephrine neurons involved in triggering MDH secretion, and administered HA inhibits MDH release by decreasing impulse-mediated noradrenergic neurotransmission through stimulation of H 2 receptors.

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Effects of Norepinephrine and Norepinephrine Agonists and Antagonists on the Melanophores of the Fiddler Crab Uca Pugilator

Cited by 39 publications

References 34 publications

Effects of the antidepressant fluoxetine on pigment dispersion in chromatophores of the common sand shrimp, Crangon crangon: repeated experiments paint an inconclusive picture

Effects of the antidepressant fluoxetine on pigment dispersion in chromatophores of the common sand shrimp, Crangon crangon: repeated experiments paint an inconclusive picture

Effect of Cadmium Chloride on the Distal Retinal Pigment Cells of the Fiddler Crab, Uca pugilator

INHIBITORY EFFECT OF HISTAMINE ON THE RELEASE OF MELANIN-DISPERSING HORMONE IN THE FIDDLER CRAB,UCA PUGILATOR

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