Effects of norfloxacin on nitrate reduction and dynamic denitrifying enzymes activities in groundwater

Chen, Linpeng; Huang, Fujian; Zhang, Chong; Zhang, Jia; Liu, Fei; Guan, Xiangyu

doi:10.1016/j.envpol.2021.116492

Cited by 41 publications

(9 citation statements)

References 47 publications

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“…The denitrifying enzyme activities are closely linked to substrate concentrations ( 30 ). Dynamic changes in substrate concentrations lead to variation in denitrifying enzyme activities, and the sensitivities of denitrifying enzymes activities to environmental conditions are different with different substrate concentrations ( 31 ). It has been shown that nitrate reduction might be the rate-limiting step in the denitrification process ( 32 ), so it was confirmed that high florfenicol concentrations hindered the water denitrification process and inhibited nitrate reductase activity in our study.…”

Section: Discussionmentioning

confidence: 99%

Effect of florfenicol on nirS-type denitrifying communities structure of water in an aquatic microcosm model

et al. 2023

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Florfenicol is used worldwide for its low side effects and strong bactericidal effect. Florfenicol is physicochemically stable and can persist in natural water bodies and affect water denitrification. Indoor aquatic microcosm models were constructed and water samples were collected at different florfenicol concentrations (0.1, 1, 10, and 100 mg/L) on days 0, 7, 30, and 60 to extract the microbial genome DNA and determine the water properties. qPCR and amplicon sequencing were used to study the dynamic changes of nirS gene and nirS-type denitrifying communities structure, diversity and abundance, respectively. The results showed that higher florfenicol concentrations caused accumulation of nitrate and ammonium nitrogen in water. Florfenicol stress caused orders of magnitude changes in nirS gene abundance, showing a trend of increasing first and then decreasing. 100 mg/L florfenicol addition led to a sustained increase of nirS gene abundance in water bodies. The florfenicol addition altered denitrifying community structure and suppressed the richness and diversity index of denitrifying bacteria in water body. Over time, the richness and diversity index gradually recovered. Proteobacteria was always the dominant denitrifying phylum in water. The relative abundance of Pseudomonas and beta proteobacterium showed obvious positive correlation with nirS gene abundance and were the dominant genera under florfenicol stress. Our study provided a scientific basis for the rational use of florfenicol in aquaculture to maintain a healthy and stable microecological environment, and also provided a preliminary understanding of the response characteristics of water denitrifying microorganisms to florfenicol exposure.

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Section: Discussionmentioning

confidence: 99%

Effect of florfenicol on nirS-type denitrifying communities structure of water in an aquatic microcosm model

et al. 2023

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“…At the beginning of the tests, DO is present in the test suspension and there is an air headspace in the test bottles. Thus, in order to stimulate the establishment of anoxic conditions, purging with argon or helium is proposed in the literature (Ilhan et al 2011;Hou et al 2015;Chen et al 2021). We compared the development of the DO concentrations and the denitrification rates in samples purged with argon, with initial DO concentrations of 0.5 mg L -1 , to those without purging.…”

Section: Evaluation Of the Sba And Lsa Optimizationmentioning

confidence: 99%

“…While lab-scaled denitrifying bioreactors enable a good insight into the processes under near-natural flowing conditions (Gottschall et al 2016;Tseng et al 2020;Lepine et al 2021), their construction and maintenance are time-consuming, costly, and need sufficient space often not available in commercial laboratories. Laboratory batch experiments provide an effective, low-effort, and low-cost alternative for studying denitrification in bioreactors (Ilhan et al 2011;Chen et al 2021). However, so far, no consistent and standardized method exists which enables comparisons and generalizations of results from different xenobiotics studies.…”

Section: Introductionmentioning

confidence: 99%

Denitrification assays for testing effects of xenobiotics on aquatic denitrification and their degradation in aquatic environments

Pániková

Weigelhofer

Bílková

et al. 2023

Preprint

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We developed, tested, and optimized two laboratory denitrification assays for both managers and scientists to assess the effects of xenobiotics on the denitrification process over 7 days (short batch assay, SBA) and 28 days (long semi-continuous assay, LSA). The assays facilitate (1) measuring the efficiency of the nitrate removal under the influence of xenobiotics, (2) determining the removal of the tested xenobiotic via adsorption or biotic decomposition, and (3) testing influencing parameters for optimizing the denitrification process. The adsorption of the xenobiotic was assessed by inhibiting all biological processes through the addition of HgCl2. Our tests demonstrate that the ratio of the initial nitrate concentration to the amount of bioavailable organic matter provided is essential to avoid organic carbon or nitrate limitation. While pH < 7 resulted in decreased denitrification, pH > 8 led to nitrite accumulation, indicating incomplete denitrification. Over durations of more than a week, weekly replenishments of the nitrate and the HgCl2, and weekly purging with argon gas to reduce oxygen concentrations are needed. The assays provide information about the accumulation of xenobiotics in the bioreactors necessary for an environmentally friendly treatment of the bioreactor fillings and provide insight into their potential to remove pesticides from polluted water resources.

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“…Antibiotic drug residues are released to the environment through WWTP and can affect the efficiency of the microbial communities essential to CW function. Antibiotics inhibit some types of bacterial growth [ 166 ], impact denitrifying bacteria [ 167 , 168 ] and potentially alter the denitrification rate [ 169 ]. Remediation of wastewater containing a high percentage of nitrate utilizes biological denitrification, where CW plays a significant role [ 170 ].…”

Section: Review Findingsmentioning

confidence: 99%

Performance Efficiency of Conventional Treatment Plants and Constructed Wetlands towards Reduction of Antibiotic Resistance

Hazra

Durso

2022

Antibiotics

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Domestic and industrial wastewater discharges harbor rich bacterial communities, including both pathogenic and commensal organisms that are antibiotic-resistant (AR). AR pathogens pose a potential threat to human and animal health. In wastewater treatment plants (WWTP), bacteria encounter environments suitable for horizontal gene transfer, providing an opportunity for bacterial cells to acquire new antibiotic-resistant genes. With many entry points to environmental components, especially water and soil, WWTPs are considered a critical control point for antibiotic resistance. The primary and secondary units of conventional WWTPs are not designed for the reduction of resistant microbes. Constructed wetlands (CWs) are viable wastewater treatment options with the potential for mitigating AR bacteria, their genes, pathogens, and general pollutants. Encouraging performance for the removal of AR (2–4 logs) has highlighted the applicability of CW on fields. Their low cost of construction, operation and maintenance makes them well suited for applications across the globe, especially in developing and low-income countries. The present review highlights a better understanding of the performance efficiency of conventional treatment plants and CWs for the elimination/reduction of AR from wastewater. They are viable alternatives that can be used for secondary/tertiary treatment or effluent polishing in combination with WWTP or in a decentralized manner.

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Effects of norfloxacin on nitrate reduction and dynamic denitrifying enzymes activities in groundwater

Cited by 41 publications

References 47 publications

Effect of florfenicol on nirS-type denitrifying communities structure of water in an aquatic microcosm model

Effect of florfenicol on nirS-type denitrifying communities structure of water in an aquatic microcosm model

Denitrification assays for testing effects of xenobiotics on aquatic denitrification and their degradation in aquatic environments

Performance Efficiency of Conventional Treatment Plants and Constructed Wetlands towards Reduction of Antibiotic Resistance

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