Effects of norspermidine on <i>Pseudomonas aeruginosa</i> biofilm formation and eradication

Q, Lin; She, Pengfei; Wang, Yangxia; Liu, Fengxia; Zhang, Di; Chen, Lihua; Luo, Zhen; Xu, Huan; Qi, Yong; Wu, Yong

doi:10.1002/mbo3.338

Cited by 70 publications

(48 citation statements)

References 53 publications

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“…The oligonucleotide primers used to amplify the QS‐related genes ( lasR , rhlR , mvfR , and pqsC/D ) and EPS‐related genes ( pslA , pelA , and alg44 ) were referred to Qu et al. () and Kim, Park, and Lee (), respectively.…”

Section: Methodsmentioning

confidence: 99%

Meloxicam inhibits biofilm formation and enhances antimicrobial agents efficacy by Pseudomonas aeruginosa

et al. 2017

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Microbial biofilms are communities of surface‐adhered cells enclosed in a matrix of extracellular polymeric substances. Bacterial cells in biofilm are 10~1,000‐fold more resistant to antimicrobials than the planktonic cells. Burgeoning antibiotic resistance in Pseudomonas aeruginosa biofilm has necessitated the development of antimicrobial agents. Here, we have investigated the antibiofilm effect of meloxicam against P. aeruginosa PAO1 and its potential mechanisms. Further, we have explored whether meloxicam could enhance the susceptibility of bacterial biofilms to treatment with conventional antimicrobials. Here, we found that meloxicam could significantly inhibit PAO1 biofilm formation in a dose‐dependent manner at the concentration without influence on planktonic cell growth. Meloxicam could also significantly inhibit the motilities, production of extracellular matrix, and expression of quorum sensing‐related genes and virulence factors of PAO1. Furthermore, synergistic interaction was observed when meloxicam combined with tetracycline, gentamicin, tobramycin, ciprofloxacin, ceftriaxone, ofloxacin, norfloxacin, ceftazidime, and DNase at subminimal inhibitory concentrations against PAO1 bioiflm. Collectively, our study lays the foundation for further investigation of repurposing meloxicam as a topical antibiofilm agent to treat P. aeruginosa biofilm‐related infections.

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Section: Methodsmentioning

confidence: 99%

Meloxicam inhibits biofilm formation and enhances antimicrobial agents efficacy by Pseudomonas aeruginosa

et al. 2017

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“…This led to reduced attachment to the surface. A smaller number of cells attached to the surface resulted in limited biofilm production and easier eradication (Qu et al 2016). Biofilm formation can be also prevented by applying the analogues of AHL molecules that inhibit QS communication.…”

Section: Mechanisms Of Qs Inhibition-qqmentioning

confidence: 99%

Prevention of biofilm formation by quorum quenching

Paluch

Rewak‐Soroczyńska

Jędrusik

et al. 2020

Appl Microbiol Biotechnol

304

151

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Quorum sensing (QS) is a mechanism that enables microbial communication. It is based on the constant secretion of signaling molecules to the environment. The main role of QS is the regulation of vital processes in the cell such as virulence factor production or biofilm formation. Due to still growing bacterial resistance to antibiotics that have been overused, it is necessary to search for alternative antimicrobial therapies. One of them is quorum quenching (QQ) that disrupts microbial communication. QQ-driving molecules can decrease or even completely inhibit the production of virulence factors (including biofilm formation). There are few QQ strategies that comprise the use of the structural analogues of QS receptor autoinductors (AI). They may be found in nature or be designed and synthesized via chemical engineering. Many of the characterized QQ molecules are enzymes with the ability to degrade signaling molecules. They can also impede cellular signaling cascades. There are different techniques used for testing QS/QQ, including chromatography-mass spectroscopy, bioluminescence, chemiluminescence, fluorescence, electrochemistry, and colorimetry. They all enable qualitative and quantitative measurements of QS/QQ molecules. This article gathers the information about the mechanisms of QS and QQ, and their effect on microbial biofilm formation. Basic methods used to study QS/QQ, as well as the medical and biotechnological applications of QQ, are also described. Basis research methods are also described as well as medical and biotechnological application.

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“…Since the field first picked up in 1994, there has been an exponential increase in publication output in the field leading up to 2018, with particularly dramatic increases seen over the past 3–4 years. This is due to the pronounced ability of P. aeruginosa to induce biofilm‐related disease that is causing significant and ongoing concern in the clinical setting, which has spurred increasing research effort in developing strategies that target their control and eradication (Qu et al, 2016). In our study, significant bibliographic coupling was found for publications arising from 820 institutions over 57 countries worldwide, among the almost ten thousand publications in the field that have appeared since 1994.…”

Section: Discussionmentioning

confidence: 99%

Global trends of Pseudomonas aeruginosa biofilm research in the past two decades: A bibliometric study

Zhu

Reng

et al. 2020

MicrobiologyOpen

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Pseudomonas aeruginosa biofilm formation is a primary cause of chronic infections. This has been a highly active area of research over the past two decades due to causing high mortality risks in immunocompromised patients. This study evaluates global trends in the dynamic and rapidly evolving field of P. aeruginosa biofilm research through bibliometric and visualized analyses. Publications from 1994 to 2018 on P. aeruginosa biofilm research were retrieved from Web of Science, Scopus, and PubMed, and their bibliometric data were systematically studied. The VOSviewer software was used to conduct global analyses of bibliographic coupling, coauthorship, cocitation, and co‐occurrence. A total of 9,527 publications were included in this study. The overall number of publications and research interest in the field displayed a strongly rising trend. The USA made the greatest contributions to the field, with the highest h‐index and number of citations compared with other countries, while Denmark had the highest average citation per publication. The Journal of Bacteriology had the highest number of publications in the field, while the University of Copenhagen was the institution with the highest contribution influence. Co‐occurrence network maps revealed that the most prominent topics in P. aeruginosa biofilm research were mechanistic studies, in vitro/in vivo studies, and biofilm formation studies. Pseudomonas aeruginosa biofilms constitute a dynamic research area in microbiology with increasing global research interest. Future studies will likely focus on investigating the mechanisms of biofilm formation to solve infection‐associated clinical problems.

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Effects of norspermidine on Pseudomonas aeruginosa biofilm formation and eradication

Cited by 70 publications

References 53 publications

Meloxicam inhibits biofilm formation and enhances antimicrobial agents efficacy by Pseudomonas aeruginosa

Meloxicam inhibits biofilm formation and enhances antimicrobial agents efficacy by Pseudomonas aeruginosa

Prevention of biofilm formation by quorum quenching

Global trends of Pseudomonas aeruginosa biofilm research in the past two decades: A bibliometric study

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