Effects of oestradiol-17 , progesterone or bovine follicular fluid on the plasma concentrations of FSH and LH in ovariectomized Booroola ewes which were homozygous carriers or non-carriers of a fecundity gene

McNatty, Kenneth P.; Hudson, N. L.; Collins, Fraser L.; Fisher, Michael B.; Heath, D. A.; Henderson, Kathryn

doi:10.1530/jrf.0.0870573

Cited by 27 publications

(26 citation statements)

References 8 publications

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“…Previous studies [5,[25][26][27][28][29] have shown that plasma concentrations of FSH at some stages of the estrus cycle are higher in prolific breeds than in nonprolific breeds. Our results confirm this and show that there were fewer oocytes and follicles in the Yunling black goat compared with the Boer goat, suggesting that FSH level is directly associated with litter size.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Expression Levels of Genes Relating to the Ovulation Rate of the Yunling Black Goat1

Cui

Zhao

Cheng

et al. 2009

Biology of Reproduction

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This study investigated the nucleotide sequences and tissue expression levels of genes relating to the ovulation rate in Yunling black goats, a famous Yunnan province, China, local breed with low fecundity. Five genes, FSHB, FSHR, BMP15, BMPR1B, and ESR2, were investigated; the complete cDNA sequences of these genes were 390, 2088, 1185, 1509, and 1585 bp, respectively, and compared with Boer goats (a more fecund breed), the sequence identities were 99%, 99%, 99%, 100%, and 99%, respectively. There were two base differences in FSHB and BMP15, four in FSHR, and three in ESR2. There were fewer follicles and oocytes in Yunling black goats than in Boer goats. Expression levels of FSHB, FSHR, and BMP15 genes in Yunling black goats were lower, and expression levels of BMPR1B and ESR2 genes were higher. Serum FSH content was lower in Yunling black goats, but serum estrogen content was higher. Protein expression levels of FSHR, BMP15, BMPR1B, and ESR2 in ovary and pituitary correlated positively with gene mRNA expression levels. In Yunling black goats, the mRNA expression levels of FSHB, FSHR, and BMP15 positively correlated with litter size, but those of BMPR1B and ESR2 correlated negatively. Together, base changes and variations in mRNA and protein expression levels of genes relating to the ovulation rate result in low fecundity in the Yunling black goat. Reduced BMP15 and FSHR levels may be related to the observed fewer oocytes

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Section: Discussionmentioning

confidence: 99%

Cloning and Expression Levels of Genes Relating to the Ovulation Rate of the Yunling Black Goat1

Cui

Zhao

Cheng

et al. 2009

Biology of Reproduction

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“…Booroola ewes have been reported to have up to 25% higher progesterone concentrations than ordinary Merino ewes during the midluteal phase of the estrous cycle, but this difference was not significant [12] because of the small sample size. In the follicular phase of the estrous cycle induced by prostaglandin F 2␣ , there were no gene-specific differences in concentrations of progesterone [13][14][15]. No differences were found in ovarian secretion rates of immunoreactive inhibins around luteolysis or in daily plasma concentrations throughout the estrous cycle, as measured by the Monash RIA [14].…”

Section: Introductionmentioning

confidence: 91%

“…More recently, plasma inhibin A concentration was determined using a specific two-site ELISA during the follicular and early luteal phases in ewes with an ovarian autotransplant, but no differences were noted between genotypes [13]. FSH and LH concentrations in plasma were not different between genotypes after treatment of ovariectomized ewes with estradiol, progesterone, or bovine follicular fluid, indicating the sensitivity of the hypothalamic-pituitary axis to ovarian hormones are similar for Booroola and ordinary Merino ewes [15].…”

Section: Introductionmentioning

confidence: 99%

Concentrations of Progesterone, Follistatin, and Follicle-Stimulating Hormone in Peripheral Plasma Across the Estrous Cycle and Pregnancy in Merino Ewes That Are Homozygous or Noncarriers of the Booroola Gene1

Xia¹,

O’Shea²,

Murison³

et al. 2003

Biology of Reproduction

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The circulating concentrations of progesterone, FSH, and follistatin across the estrous cycle and gestation were compared in Australian merino sheep that were homozygous for the Booroola gene, FecB, or were noncarriers. The Booroola phenotype is due to a point mutation in the bone morphogenetic protein receptor 1B. Progesterone concentrations began to rise earlier and were higher in the Booroola ewes than in the noncarriers on most days of the luteal phase but not during the follicular phase of the cycle. Follistatin concentrations remained unchanged across the estrous cycle in both groups of ewes, with no differences between genotypes. FSH concentrations were higher in Booroola ewes than in noncarrier ewes on most days of the estrous cycle, with a significantly higher and broader peak of FSH around the time of estrus. Progesterone concentrations were significantly higher in early and midgestation in Booroola ewes but were lower toward the end of gestation than those in noncarriers. FSH declined in both groups across gestation, with lower concentrations of FSH in Booroola ewes during midgestation. Follistatin remained unchanged across gestation in Booroola ewes and noncarrier ewes with a twin pregnancy but declined across gestation in noncarrier ewes with a singleton pregnancy. These results suggest that follistatin concentration is not regulated by the FecB gene during the estrous cycle and pregnancy but is influenced by the number of fetuses. However, the FecB gene appears to positively affect both progesterone and FSH during the estrous cycle and across pregnancy, which suggests that bone morphogenetic proteins play an important role in the regulation of both hormones.

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“…Rats received a single implant of approximately 1.8 cm with an internal diameter of 3.35 mm (Chen et al 2001). Ewes received 2 implants based on previous results with implants containing oestradiol (McNatty et al 1989). Oocytes and follicular debris were removed from the cells using a micro-glass pipette.…”

Section: Collection Of Granulosa Cellsmentioning

confidence: 99%

The role of bone morphogenetic proteins 2, 4, 6 and 7 during ovarian follicular development in sheep: contrast to rat

Juengel¹,

Reader²,

Bibby³

et al. 2006

Reproduction

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The intraovarian roles of BMP family members such as BMP2, 4, 6 and 7 are not well understood, particularly in species with low ovulation rates such as sheep. Therefore, the objectives of these experiments were to determine the expression patterns of mRNAs encoding BMP2, 4, 6 and 7 during ovarian follicular development in sheep, and to determine the effects of these growth factors on ovine granulosa cell functions in vitro. For comparative purposes, the effects of these BMPs were also determined in rat granulosa cells since these factors have been most widely studied in this poly-ovulatory species. As assessed by in situ hybridization, non-atretic ovine follicles expressed mRNA for BMP6 but not 2, 4 or 7. Furthermore, expression of BMP6 was limited to the oocyte of primordial as well as primary, pre-antral and antral follicles. Reverse transcription-PCR of granulosa cell mRNA detected low levels of all the BMPs in some pools of cells. BMP2, 4, 6 and 7 each inhibited progesterone production from ovine granulosa cells without affecting cellular proliferation/survival. Similarly, these BMPs inhibited progesterone production from rat granulosa cells. However, they also stimulated cellular proliferation/survival of the rat granulosa cells highlighting a species-specific difference for these growth factors. In conclusion, in sheep, BMP2, 4, 6 and 7 inhibit granulosa cell differentiation without affecting proliferation. However, as BMP2, 4 and 7 were not detectable by in situ hybridization in any cells of non-atretic ovarian follicles, it seems unlikely that these proteins would have an important intraovarian role in regulating follicular development in sheep. In contrast, localization of BMP6 mRNA in the oocyte suggests that this BMP family member may have a paracrine and/or autocrine role in regulating follicular growth in sheep, as has been shown for two other oocyte derived from members of the transforming growth factor superfamily, BMP15 and growth differentiation factor 9.

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Effects of oestradiol-17 , progesterone or bovine follicular fluid on the plasma concentrations of FSH and LH in ovariectomized Booroola ewes which were homozygous carriers or non-carriers of a fecundity gene

Cited by 27 publications

References 8 publications

Cloning and Expression Levels of Genes Relating to the Ovulation Rate of the Yunling Black Goat1

Cloning and Expression Levels of Genes Relating to the Ovulation Rate of the Yunling Black Goat1

Concentrations of Progesterone, Follistatin, and Follicle-Stimulating Hormone in Peripheral Plasma Across the Estrous Cycle and Pregnancy in Merino Ewes That Are Homozygous or Noncarriers of the Booroola Gene1

The role of bone morphogenetic proteins 2, 4, 6 and 7 during ovarian follicular development in sheep: contrast to rat

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