Effects of on-farm supplemental feeding of probiotic &lt;i&gt;Bacillus subtilis&lt;/i&gt; on milk production in lactating dairy cows under tropical conditions

Choonkham, Watcharapong; Schonewille, J.T.; Bernard, J.K.; Suriyasathaporn, Witaya

doi:10.22358/jafs/127692/2020

Cited by 9 publications

(9 citation statements)

References 27 publications

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“…Consequently, we can assume that the inclusion of multicomponent synbiotic “Kormomix® Rumin” had no impact on milk productivity parameters compared with the control group. Our results come in the opposite direction from those obtained by ( 27 ), who reported that milk yield increased in response to supplementation of B. subtilis . They mentioned that cows fed 2 × 10 11 CFU of B. subtilis daily produced 1.7 kg more milk than the control group.…”

Section: Discussioncontrasting

confidence: 99%

“…Thus, we conclude that the inclusion of multicomponent synbiotic “Kormomix® Rumin” in diets of Russian Holstein dairy cows, in general, did not deteriorate the blood characteristics. Our results match those obtained by ( 27 ); they mentioned that hematological and biochemical parameters did not vary between dairy cows fed different treatments of B. subtilis .…”

Section: Discussionsupporting

confidence: 91%

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Impact of Inclusion of Multicomponent Synbiotic Russian Holstein Dairy Cow's Rations on Milk Yield, Rumen Fermentation, and Some Blood Biochemical Parameters

et al. 2022

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The purpose of this study was to appraise the effect of the inclusion of multicomponent synbiotic “Kormomix® Rumin” in feeding lactating Holstein cows on milk productivity, indicators of rumen fermentation metabolism, and some hematological and biochemical parameters of the blood. For this study, 40 highly productive Russian Holstein cows were selected according to their productivity, physiological condition, live weight, and age. They were divided into four groups (10 heads/each). All animals received the basal total mixed ration (TMR), which was balanced and corresponded to the nutritional requirements for cows during the milking period with a milk yield of 36 kg/daily. The first group (control) fed basal (TMR) only while the 2nd, 3rd, and 4th group fed the basal (TMR) supplemented with a multicomponent synbiotic “Kormomix® Rumin” in amounts 25, 50, and 75 g/head/day, respectively, which was administered manually and individually after morning feeding daily and mixing carefully together with the concentrates directly after calving until 120 DIM. Milk, ruminal fluid, and blood samples were collected for studying the studied parameters. The highest values in all studied milk parameters were recorded in the 2nd experimental group but the differences were not significant. The inclusion of “Kormomix® Rumin” increased significantly the synthesis of volatile fatty acids in the 2nd experimental group (9.38 vs. 7.04 mmol/100 ml) in the control group. The level of serum α-Amylase (total) decreased significantly in the 2nd experimental group compared with other groups. The urea level recorded the lowest value in the control group, while the urea/creatinine ratio recorded the lowest value in the 4th group and the differences were significant when compared with the 2nd group. Accordingly, the inclusion of synbiotic “Kormomix® Rumin” in the diets of lactating cows has no impact on milk production. Whereas, it improves the intensity of rumen fermentation, which contributes to more efficient utilization of feed without any harmful effects on blood traits. Moreover, the recommended dose for use in their diets is 25 g/head/day.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 91%

Impact of Inclusion of Multicomponent Synbiotic Russian Holstein Dairy Cow's Rations on Milk Yield, Rumen Fermentation, and Some Blood Biochemical Parameters

et al. 2022

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“…Bacillus -based DFMs are already in use in several monogastric animal species, with positive impacts on nutrient absorption and in immune response ascribed to the alteration of the microbial ecology in the digestive tract [ 17 , 18 ]. B. subtilis -based DFM have also been applied to dairy cattle, in which they consistently exerted a positive influence on ruminal fermentation, growth, lactation performance, and milk composition [ 19 , 20 , 21 , 22 , 23 , 24 ]. Yet, the bacterial functions that are at the basis of such beneficial effect still await elucidation [ 9 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

An In Vitro Study on the Role of Cellulases and Xylanases of Bacillus subtilis in Dairy Cattle Nutrition

Bontà,

Battelli,

Rama

et al. 2024

Microorganisms

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The administration of Bacilli to dairy cows exerts beneficial effects on dry matter intake, lactation performance, and milk composition, but the rationale behind their efficacy is still poorly understood. In this work, we sought to establish whether cellulases and xylanases, among the enzymes secreted by B. subtilis, are involved in the positive effect exerted by Bacilli on ruminal performance. We took advantage of two isogenic B. subtilis strains, only differing in the secretion levels of those two enzymes. A multi-factorial study was conducted in which eight feed ingredients were treated in vitro, using ruminal fluid from cannulated cows, with cultures of the two strains conveniently grown in a growth medium based on inexpensive waste. Feed degradability and gas production were assessed. Fiber degradability was 10% higher (p < 0.001) in feeds treated with the enzyme-overexpressing strain than in the untreated control, while the non-overexpressing strain provided a 5% increase. The benefit of the fibrolytic enzymes was maximal for maize silage, the most recalcitrant feed. Gas production also correlated with the amount of enzymes applied (p < 0.05). Our results revealed that B. subtilis cellulases and xylanases effectively contribute to improving forage quality, justifying the use of Bacilli as direct-fed microbials to increase animal productivity.

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“…This species, listed in the Qualified Presumption of Safety (QPS) recommended microorganisms intentionally added to food or feed (EFSA BIOHAZ Panel, 2022, 2023a, 2023b), was shown to colonize the gastrointestinal tract of mammals and poultry, in the form of vegetative cells (spores) or forming biofilms, thereby acting as probiotic (Bernardeau et al, 2017; Lee et al, 2019; Mun at al., 2021). In particular, B. subtilis spores added to dairy cattle feed consistently exerted a positive influence on ruminal fermentation, growth, lactation performance, and milk composition (Sun et al, 2011; Sun et al, 2013; Sun et al, 2016; Souza et al, 2017; Choonkham et al, 2020; Wang et al, 2022; Jia et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

The importance of cellulolytic and xylanolytic activities ofBacillus subtilison dairy cow feed digestibility

Bontà¹,

Battelli

Rama³

et al. 2023

Preprint

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A large body of literature has accumulated on the beneficial impact of the administration ofBacillito dairy cows, particularly on dry matter intake, lactation performances and milk composition. In this work we sought to establish whether the ability ofB. subtilisto secrete cellulase and xylanase enzymes could be involved in the positive effect exerted by these bacteria. Several feed ingredients were treated with twoB. subtilisstrains only differing for the amount of secreted cellulosolytic and xylanolytic enzymes, and feed quality was assessed. We found that in vitro fibre digestibility correlated with the activity of those enzymes. Our results revealed thatB. subtiliscellulases and xylanases can effectively improve forage quality, providing a scientific rationale on the use of Bacilli as forage supplements to improve animal productivity. Moreover, a particular care was taken in designing a sustainable and economically viableBacillus-based additive preparation process.

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Effects of on-farm supplemental feeding of probiotic <i>Bacillus subtilis</i> on milk production in lactating dairy cows under tropical conditions

Cited by 9 publications

References 27 publications

Impact of Inclusion of Multicomponent Synbiotic Russian Holstein Dairy Cow's Rations on Milk Yield, Rumen Fermentation, and Some Blood Biochemical Parameters

Impact of Inclusion of Multicomponent Synbiotic Russian Holstein Dairy Cow's Rations on Milk Yield, Rumen Fermentation, and Some Blood Biochemical Parameters

An In Vitro Study on the Role of Cellulases and Xylanases of Bacillus subtilis in Dairy Cattle Nutrition

The importance of cellulolytic and xylanolytic activities ofBacillus subtilison dairy cow feed digestibility

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