Effects of one-day reperfusion after transient forebrain ischemia on circulatory system in the rat

Abstract

Abstract. Although ischemia/reperfusion injury remains incompletely understood, it appears that reactive oxygen species produced mainly during postischemic recirculation play a critical role. The present study examined the impact of forebrain ischemia and subsequent one-day reperfusion on several blood parameters. We determined glutamate concentration in whole blood, measured Cu/Zn-and Mn-SOD (superoxide dismutase) activity in blood cells as well as plasma, and investigated the prevalence of single and double strand breaks of lymphocyte DNA. The results of our experiment showed that the concentration of glutamic acid in whole blood was increased by about 25%. Antioxidant activity of total SOD and Cu/Zn-SOD was reduced in blood cells and plasma. Mn-SOD activity in blood cells was not affected by ischemic insult and one-day reperfusion, but we detected its significantly lower activity in samples of plasma. We observed a weakly reduced level of double and a significantly elevated level of single strand breaks of lymphocyte DNA.In conclusion, one day of recovery after the ischemic attack failed to return peripheral circulatory system to physiological conditions. Reduced antioxidant capacity in the blood and an elevated level of excitotoxic amino acid glutamate may cause lymphocyte DNA damage, and probably contribute to insufficient postischemic recovery of brain tissue.

Blood cells serve as a source of factor‐inducing rapid ischemic tolerance in brain

“…As a first in the present experiment, we tested the hypothesis that production of the factor‐inducing tolerance of neuronal cells to ischemia depends on protein synthesis. Our previous results confirmed the rapid response of sensitive brain tissue to nutrients and oxygen insufficiency in the systemic circulation (Kravcukova et al ., , ). We observed that global as well as focal brain ischemia led to dramatic changes in several blood parameters, such as the activity of SOD enzymes and glutamate level in early postischemic reperfusion.…”

Remote Ischaemic Preconditioning Accelerates Brain to Blood Glutamate Efflux via EAATs-mediated Transport

“…Brain tissue excitotoxicity reaches beyond the border of the central nervous system. In the blood, the early stage of post-ischaemic reperfusion reflects an increase in blood glutamate level, ultimately gaining in oxidative stress and affecting the enzyme antioxidant defence [ 5 , 6 ].…”

Brain-derived neurotrophic factor blood levels in two models of transient brain ischemia in rats

“…In our previous work we observed changes of some biochemical parameters in blood in model of global (Kravcukova et al 2009(Kravcukova et al , 2010 as well as focal ischemia (unpublished data) that are clearly expressed mainly in early period of postischemic recirculation. Based on this we decided to study ischemia impact on blood BDNF during blood supply restriction and early reperfusion and examine its effect on whole blood, plasma and blood cells.…”