Effects of oral administration of l-arginine, l-NAME and allopurinol on intestinal ischemia/reperfusion injury in rats

Margaritis, E; Yanni, Amalia E.; Agrogiannis, George; Liarakos, Nikolaos; Pantopoulou, Alkistis; Vlachos, Ioannis S.; Papachristodoulou, A; Korkolopoulou, P.; Patsouris, Efstratios; Kostaki, Maria; Perrea, Despina; Kostakis, Alkiviadis

doi:10.1016/j.lfs.2011.04.009

Cited by 18 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…XOD is considered the most important source of oxygen free radicals (28). Meanwhile, living tissues are endowed with innate antioxidant defense mechanisms, namely antioxidative enzymes.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of endogenous hydrogen sulfide against oxidative stress in gastric ischemia-reperfusion injury

Cui

Liu

Zou

et al. 2012

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Hydrogen sulfide (H2S) is a gaseous signaling molecule, which plays a critical role in a number of physiological and pathological progresses. In order to determine the effect of endogenous H2S on gastric ischemia-reperfusion (GI-R), we evaluated the gastric mucosal damage in rats intraperitoneally injected with DL-propargylglycine (PAG, 50 mg/kg/day) or L-cysteine (L-cys, 50 mg/kg/day) for 7 days before GI-R. GI-R injury was achieved by clamping the celiac artery for 30 min, followed by reperfusion for 60 min. Gastric mucosal damage was macroscopically assessed in the area of injury and deep damage was assessed by histopathological scoring. PAG increased the area of gastric mucosal injury and deep damage compared with that in untreated GI-R rats (P<0.05). While PAG decreased the H2S concentration and cystathionine γ-lyase (CSE) expression in the gastric mucosa, L-cys significantly attenuated the effects of GI-R. Western blot analysis revealed that the increases of malondialdehyde (MDA) and xanthine oxidase (XOD), and decreases of glutathione (GSH), superoxide dismutase (SOD) and the restriction of superoxide (O2−) production in the PAG group were inhibited by L-cys (P<0.05). Endogenous H2S has a protective effect against GI-R in rats by inhibiting oxygen free radical overproduction.

show abstract

“…XOD is considered the most important source of oxygen free radicals (28). Meanwhile, living tissues are endowed with innate antioxidant defense mechanisms, namely antioxidative enzymes.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of endogenous hydrogen sulfide against oxidative stress in gastric ischemia-reperfusion injury

Cui

Liu

Zou

et al. 2012

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…Anti-inflammatory effects of allopurinol decreasing inflammatory enzyme activities (MPO and NAG) have been reported in the ischemia/reperfusion model in rats and in dogs (Margaritis et al, 2011;Peto et al, 2005) and cytokines production (CCL2 and TNF-α levels), the systemic levels of TNF-α were shown to be reduced by preventive administration of allopurinol (300 mg/kg) in patients submitted to cardiac bypass surgery (Gormus et al, 2013). In 2011 Baldwin and colleagues demonstrated that uric acid induced in vitro an increase in the production (mRNA and secreted protein) of chemokine (C-C motif) ligand 2 (CCL2) (Baldwin et al, 2011).…”

Section: Discussionmentioning

confidence: 98%

“…However, more recent data have indicated the use of xanthine oxidase inhibitors to various Microvascular Research 101 (2015) 118-126 forms of ischemia and other types of tissue and vascular injuries, inflammatory diseases, chronic heart failure (Berry and Hare, 2004;Harrison, 2002;Harrison, 2004). Allopurinol and its active metabolite oxypurinol has shown considerable promise in the treatment of these conditions both in experimental animals and in small-scale human clinical trials (Aldaba-Muruato et al, 2013;Khanna and FitzGerald, 2015;Kim et al, 2015;Margaritis et al, 2011;Pacher et al, 2006;Peto et al, 2005). The main mechanisms by which allopurinol exerts its beneficial effects involve decreased generation of superoxide, hydrogen peroxide and uric acid.…”

Section: Introductionmentioning

confidence: 99%

Angiopreventive versus angiopromoting effects of allopurinol in the murine sponge model

Orellano

Almeida

Campos

et al. 2015

Microvascular Research

View full text Add to dashboard Cite

“…Inhibition of XO by the purine analog, allopurinol, protects the intestine from I/R injury. ( 71 – 73 )…”

Section: Oxidative Stressmentioning

confidence: 99%

Microbiome and intestinal ischemia/reperfusion injury

Nadatani

Watanabe

Shimada

et al. 2018

J. Clin. Biochem. Nutr.

View full text Add to dashboard Cite

Intestinal ischemia/reperfusion injury is a severe disease associated with a high mortality. The mechanisms that cause ischemia/reperfusion injury are complex and many factors are involved in the injury formation process; however, the only available treatment is surgical intervention. Recent studies demonstrated that the intestinal microbiome plays a key role in intestinal ischemia/reperfusion injury and there are many factors associated with intestinal bacteria during the formation of the intestinal ischemia/reperfusion injury. Among the Toll-like receptors (TLR), TLR2, TLR4, and their adaptor protein, myeloid differentiation primary-response 88 (MyD88), have been reported to be involved in intestinal ischemia/reperfusion injury. Oxidative stress and nitric oxide are also associated with intestinal bacteria during the formation of the intestinal ischemia/reperfusion injury. This review focuses on our current understanding of the impact of the microbiome, including the roles of the TLRs, oxidative stress, and nitric oxide, on intestinal ischemia/reperfusion injury.

show abstract

Effects of oral administration of l-arginine, l-NAME and allopurinol on intestinal ischemia/reperfusion injury in rats

Cited by 18 publications

References 31 publications

Protective effect of endogenous hydrogen sulfide against oxidative stress in gastric ischemia-reperfusion injury

Protective effect of endogenous hydrogen sulfide against oxidative stress in gastric ischemia-reperfusion injury

Angiopreventive versus angiopromoting effects of allopurinol in the murine sponge model

Microbiome and intestinal ischemia/reperfusion injury

Contact Info

Product

Resources

About