Effects of orthodontic force magnitude on cell apoptosis and RANKL-induced osteoclastogenesis

Kaya, Şennur; Çifter, Muhsin; Çekici, Ali; Olgaç, Vakur; İşsever, Halim; Işık, Göknur

doi:10.1007/s00056-019-00205-6

Cited by 9 publications

(15 citation statements)

References 27 publications

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“…Alveolar bone remodeling under orthodontic force is affected by the relative balance between osteoblastogenesis and osteoclastogenesis. The role of osteoclasts in bone resorption on the pressure side is relatively well known 2,3) ; however, the specific role of osteoblasts has yet to be elucidated. BMSCs are the precursors of osteoblasts and have the potential for self-renewal and multi-directional differentiation; these cells play an important role in bone formation and bone regeneration 28) .…”

Section: Discussionmentioning

confidence: 99%

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Alterations between Autophagy and Apoptosis in Alveolar Bone Mesenchymal Stem Cells under Orthodontic Force and Their Effects on Osteogenesis

Zhu

Wenrui

Zhang

et al. 2022

J. hard tissue biol.

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The aim was to investigate changes between autophagy and apoptosis in alveolar bone mesenchymal stem cells (BMSCs) under orthodontic pressure and the effect on osteogenesis in BMSCs. Twenty-four beagle canine teeth were used as experimental models. The control group received no treatment, while in the experimental groups, the canines were moved distally with 150 g of orthodontic force for 7 days, 14 days and 28 days. Bone remodeling was detected by H&E staining, while autophagy and apoptosis in the alveolar bone were analyzed by immunohistochemistry. 3-MA and Z-VAD-FMK were used to modulate autophagy and apoptosis. Transmission electron microscopy and western blotting were used to analyze autophagy and apoptosis in BMSCs, while their osteogenesis was analyzed by ALP staining, alizarin red staining, and osteogenic gene analysis. Compared to the control group, we observed significant distal movement of the canines in each of the experimental groups, the distance moved as the loading time was extended. In the experimental groups, the alveolar bone on the pressure side was clearly absorbed. On the pressure side, teeth were positive for LC3 after loading for 7 days and 14 days, whereas teeth were positive for caspase-3 after loading for 14 days and 28 days. The number of autophagosomes and the conversion of LC3-II/LC3-I gradually decreased, nevertheless, the number of apoptotic bodies and the expression of caspase-3 increased gradually. ALP activity, mineralized nodule formation, and osteogenic gene expression in BMSCs gradually decreased. Finally, the down-regulation of autophagy inhibited osteogenesis in BMSCs, whereas the restriction of apoptosis led to adverse consequences. Collectively, these data indicate that autophagy of the alveolar bone and BMSCs under orthodontic pressure gradually decreased as the loading time was extended, in contrast, the extent of apoptosis gradually increased; the osteogenic differentiation of BMSCs was positively correlated with autophagy but negatively correlated with apoptosis.

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Section: Discussionmentioning

confidence: 99%

“…Autophagy and apoptosis are both important cytological processes that occur during orthodontic tooth movement and regulate alveolar bone resorption on the pressure side [1][2][3][4] . Studies have shown that osteocyte apoptosis on an orthodontic pressure side is one of the most important mechanisms that lead to bone resorption 4) .…”

Section: Introductionmentioning

confidence: 99%

Alterations between Autophagy and Apoptosis in Alveolar Bone Mesenchymal Stem Cells under Orthodontic Force and Their Effects on Osteogenesis

Zhu

Wenrui

Zhang

et al. 2022

J. hard tissue biol.

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“…It is worth noting that, in terms of diet, a standard diet with feed pellets should be softened to mash. 28,29 The experiments usually last for 14 days.…”

Section: Ratsmentioning

confidence: 99%

Research progress of animal models on orthodontic tooth movement

Xie¹,

Chan²,

Huang³

2022

IJASR

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Orthodontic tooth movement animal models are important carriers for studying orthodontic tooth movement. Experimental animals that can be modeled include: rats, mice, dogs, rabbits, etc. How to select suitable animal models for different scientific problems is very important. Therefore, this paper reviews the types and characteristics of orthodontic tooth movement animal models, and focuses on the similarities and differences of rodent anatomy, modeling methods and considerations. It is found that there is a lack of unified modeling standards for orthodontic tooth movement animal models. Thus appropriate animal models should be selected according to actual needs.

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“…Recently, several histological studies on OTM have demonstrated that each tooth moves in the periodontal space, creating pressure and tension areas as a consequence of mechanical stress. e pressure maintained on the teeth is the fuze of its movement into a different position within the periodontal space, compressing in some areas while stretching it in others [2][3][4][5]. is leads to structural modifications of the periodontal ligament accompanied by alterations of the biochemical environment in the gingival crevicular fluid (GCF) [6].…”

Section: Introductionmentioning

confidence: 99%

Human Interleukin-1β Profile and Self-Reported Pain Monitoring Using Clear Aligners with or without Acceleration Techniques: A Case Report and Investigational Study

Pascoal

Gonçalves

Brandão

et al. 2022

International Journal of Dentistry

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Introduction. There is a growing demand for more aesthetic, comfortable, and faster orthodontic treatments, and clear aligners emerged as a solution to fulfill this need. However, the effectiveness of clear aligners to treat complex malocclusions is yet contentious. The use of acceleration methods could improve the efficacy of clear aligners by stimulating cells' mechanobiology through numerous pathways, but this hypothesis is still poorly explored. Objective. We aimed to monitor the release profile of an inflammatory marker-the interleukin-1β-and to evaluate its relationship with self-reported pain scores with and without the use of acceleration techniques during an orthodontic treatment requiring difficult tooth movements with clear aligners. Case Report. Here, we report a case of a 46-year-old female patient who presented functional and aesthetic complaints. Intraoral examination revealed a diminished overjet and overbite, rotation of teeth 45 and 24, absence of teeth 25, 35, and 36, buccolingual dislocation of tooth 21, a tendency to a Class III malocclusion, and a 2 mm left deviation of the lower midline. This study is divided into three stimulation phases: no stimulation, mechanical vibration stimulation, and photobiomodulation. Interleukin-1β levels in gingival crevicular fluid samples from the pressure side of six selected teeth were evaluated at four time points after the orthodontic treatment onset. Pain monitoring in those teeth was performed using a visual analogue scale at the same time points. Results. Interleukin-1β protein production peaked 24 h after treatment onset. Complex movements were associated with increased self-reported pain. Conclusion. Clear aligners show limitations in solving complex tooth movements, even when combined with acceleration. The development of customized and programmable stimulation microdevices integrated into “smart aligners,” which could be designed to specifically stimulate the direction of movement and stimulation parameters and could constitute a solution to optimize the orthodontic tooth movement with clear aligners.

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Effects of orthodontic force magnitude on cell apoptosis and RANKL-induced osteoclastogenesis

Cited by 9 publications

References 27 publications

Alterations between Autophagy and Apoptosis in Alveolar Bone Mesenchymal Stem Cells under Orthodontic Force and Their Effects on Osteogenesis

Alterations between Autophagy and Apoptosis in Alveolar Bone Mesenchymal Stem Cells under Orthodontic Force and Their Effects on Osteogenesis

Research progress of animal models on orthodontic tooth movement

Human Interleukin-1β Profile and Self-Reported Pain Monitoring Using Clear Aligners with or without Acceleration Techniques: A Case Report and Investigational Study

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