Effects of Osteogenic Induction on Mesenchymal Cells from Fetal and Maternal Parts of Human Placenta

Takahashi, Kenji; Igura, Koichi; Zhang, Xiaohong; Mitsuru, Ayako; Takahashi, Tsuneo

doi:10.3727/000000004783983918

Cited by 16 publications

(8 citation statements)

References 13 publications

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“…In support of this hypothesis, recent reports have described the identification of pluripotent or multipotent stem cells from human placenta cord blood or amniotic fluid [6–11]. Pluripotent stem cells were identified in cord blood [7], whereas multipotent mesenchymal stem cells were detected in various placental tissues [6, 9, 10]. Mesenchymal stem cells have also been isolated from amniotic fluid [11, 12].…”

Section: Introductionmentioning

confidence: 99%

Stem Cell Characteristics of Amniotic Epithelial Cells

et al. 2005

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Section: Introductionmentioning

confidence: 99%

Stem Cell Characteristics of Amniotic Epithelial Cells

et al. 2005

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“…Tissue resident progenitors that give rise to connective tissue cells in vitro have been isolated from a variety of tissues including bone marrow (BM) [1], [2], [3], [4], fat [5], [6], muscle [7], [8], placenta [9], umbilical cord [10], [11], [12], [13], [14] and fetal liver [15]. These cells are generally thought to be resident in the perivascular compartment of these tissues [16], [17], [18], [19], [20]; and are commonly called “mesenchymal stem cells” (MSCs) [21].…”

Section: Introductionmentioning

confidence: 99%

Human Mesenchymal Stem Cells Self-Renew and Differentiate According to a Deterministic Hierarchy

et al. 2009

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BackgroundMesenchymal progenitor cells (MPCs) have been isolated from a variety of connective tissues, and are commonly called “mesenchymal stem cells” (MSCs). A stem cell is defined as having robust clonal self-renewal and multilineage differentiation potential. Accordingly, the term “MSC” has been criticised, as there is little data demonstrating self-renewal of definitive single-cell-derived (SCD) clonal populations from a mesenchymal cell source.Methodology/Principal FindingsHere we show that a tractable MPC population, human umbilical cord perivascular cells (HUCPVCs), was capable of multilineage differentiation in vitro and, more importantly, contributed to rapid connective tissue healing in vivo by producing bone, cartilage and fibrous stroma. Furthermore, HUCPVCs exhibit a high clonogenic frequency, allowing us to isolate definitive SCD parent and daughter clones from mixed gender suspensions as determined by Y-chromosome fluorescent in situ hybridization.Conclusions/SignificanceAnalysis of the multilineage differentiation capacity of SCD parent clones and daughter clones enabled us to formulate a new hierarchical schema for MSC self-renewal and differentiation in which a self-renewing multipotent MSC gives rise to more restricted self-renewing progenitors that gradually lose differentiation potential until a state of complete restriction to the fibroblast is reached.

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“…It has become evident that various parts of human placenta that are normally discarded after delivery constitute valuable sources of maternal and fetal cells that exhibit stem cell-like plasticity (9,14,16,26). Particular attention has been directed to the amnion layer of the deflected part of the fetal membranes as a source of stem/progenitor cells of fetal origin.…”

Section: Introductionmentioning

confidence: 99%

Comparative Characterization of Cultured Human Term Amnion Epithelial and Mesenchymal Stromal Cells for Application in Cell Therapy

et al. 2008

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Emerging evidence suggests human amnion tissue as a valuable source of two distinct types of pluripotent cells, amnion epithelial cells (hAECs) and mesenchymal stromal cells (hAMSCs), for applications in cell replacement therapy. For some approaches, it may be necessary to culture and differentiate these cells before they can be transplanted. No systematic attempt has been yet made to determine the quantity and quality of amnion cells after isolation and culture. We looked at amnion cell isolates from 27 term placentas. Following our optimized protocol, primary yields were 6.3 x 10(6) hAECs and 1.7 x 10(6) hAMSCs per gram amnion. All 27 cases gave vital cultures of hAMSCs, while one third of cases (9 of 27) failed to give adherent cultures of hAECs. Primary cultures contained significantly more proliferating than apoptotic cells (hAECs: 16.4% vs. 4.0%; hAMSCs: 9.5% vs. 2.4%). Neither hAECs nor hAMSCs were clonogenic. They showed slow proliferation that almost stopped beyond passage 5. Microscopic follow-up revealed that hAEC morphology gradually changed towards mesenchymal phenotype over several passages. Flow cytometric characterization of primary cultures showed expression of mesenchymal progenitor markers CD73, CD90, CD105, and CD166, as well as the embryonic stem cell markers SSEA-3 and -4 on both amnion cell types. These profiles were grossly maintained in secondary cultures. Reverse transcriptase-PCR analysis exhibited transcripts of Oct-3/4 and stem cell factor in primary and secondary cultures of all cases, but no telomerase reverse transcriptase. Immunocytochemistry confirmed translation into Oct-3/4 protein in part of hAEC cultures, but not in hAMSCs. Further, both amnion cell types stained for CD90 and SSEA-4. Osteogenic induction studies with amnion cells from four cases showed significantly stronger differentiation of hAECs than hAMSCs; this capacity to differentiate greatly varied between cases. In conclusion, hAECs and hAMSCs in culture exhibit and maintain a similar marker profile of mesenchymal progenitors. hAECs were found as a less reliable source than hAMSCs and altered morphology during subculture.

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Effects of Osteogenic Induction on Mesenchymal Cells from Fetal and Maternal Parts of Human Placenta

Cited by 16 publications

References 13 publications

Stem Cell Characteristics of Amniotic Epithelial Cells

Stem Cell Characteristics of Amniotic Epithelial Cells

Human Mesenchymal Stem Cells Self-Renew and Differentiate According to a Deterministic Hierarchy

Comparative Characterization of Cultured Human Term Amnion Epithelial and Mesenchymal Stromal Cells for Application in Cell Therapy

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