Effects of outdoor and indoor cultivation on the polar lipid composition and antioxidant activity of Nannochloropsis oceanica and Nannochloropsis limnetica: A lipidomics perspective

Couto, Daniela; Conde, Tiago; Melo, Tânia; Neves, Bruno Miguel; Costa, Margarida; Cunha, P. A. C.; Guerra, Inês; Correia, Nádia; Silva, Joana T.; Pereira, Hugo; Varela, João; Silva, Joana; Domingues, Rosário; Domingues, Pedro

doi:10.1016/j.algal.2022.102718

Cited by 22 publications

(19 citation statements)

References 69 publications

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“…The fatty acid composition of C. vulgaris lipid extracts was analyzed by gas chromatography coupled to mass spectrometry (GC-MS). Fatty acid methyl esters (FAMEs) solution was prepared using alkaline trans-esterification of 60 μg of lipid extract and the addition of methyl nonadecanoate (internal standard) at 0.96 μg mL −1 , as performed routinely in our laboratory [ 15 , 16 ]. The GC-MS was performed using an Agilent Technologies 8860 GC System (Santa Clara, CA, USA) interfaced with an Agilent 5977B Mass Selective Detector (Agilent, Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Differences and Similarities in Lipid Composition, Nutritional Value, and Bioactive Potential of Four Edible Chlorella vulgaris Strains

Maurício

Couto

Lopes

et al. 2023

Foods

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The microalga Chlorella vulgaris is a popular food ingredient widely used in the industry, with an increasing market size and value. Currently, several edible strains of C. vulgaris with different organoleptic characteristics are commercialized to meet consumer needs. This study aimed to compare the fatty acid (FA) and lipid profile of four commercialized strains of C. vulgaris (C-Auto, C-Hetero, C-Honey, and C-White) using gas- and liquid-chromatography coupled to mass-spectrometry approaches, and to evaluate their antioxidant and anti-inflammatory properties. Results showed that C-Auto had a higher lipid content compared to the other strains and higher levels of omega-3 polyunsaturated FAs (PUFAs). However, the C-Hetero, C-Honey, and C-White strains had higher levels of omega-6 PUFAs. The lipidome signature was also different between strains, as C-Auto had a higher content of polar lipids esterified to omega-3 PUFAs, while C-White had a higher content of phospholipids with omega-6 PUFAs. C-Hetero and C-Honey showed a higher content of triacylglycerols. All extracts showed antioxidant and anti-inflammatory activity, highlighting C-Auto with greater potential. Overall, the four strains of C. vulgaris can be selectively chosen as a source of added-value lipids to be used as ingredients in food and nutraceutical applications for different market needs and nutritional requirements.

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Section: Methodsmentioning

confidence: 99%

Differences and Similarities in Lipid Composition, Nutritional Value, and Bioactive Potential of Four Edible Chlorella vulgaris Strains

Maurício

Couto

Lopes

et al. 2023

Foods

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“…The increased content of arachidonic and eicosapentaenoic acids after the use of N.o. extract may be a direct consequence of their significant content in these microalgaes (almost 10% and 30% for AA and EPA, respectively) [ 35 ]. However, the increase in the free DHA level may be related to the increase in phospholipase A2 activity by the C.a.…”

Section: Discussionmentioning

confidence: 99%

“…and C.a. , as previously described [ 35 ]. A total of 40 μg of the resulting lipid extracts were suspended in an eluent aliquot consisting of 95% mobile phase B (CH 3 CN/CH 3 OH (60:40, v / v ) with 5 mM ammonium acetate) and 5% mobile phase A (H 2 O/CH 3 CN/CH 3 OH (25:50:25, v / v / v ) with 5 mM ammonium acetate) and a mixture of lipid standard solutions (dMPC-0.04 μg, LPC-0.04 μg, dMPE-0.04 μg, dMPG-0.024 μg and Cer(17:0/d18:1)-0.04 μg).…”

Section: Methodsmentioning

confidence: 99%

“…Data collection was performed using the Xcalibur data system (V3.3, Thermo Fisher Scientific, Waltham, MA, USA). Identification of molecular species of polar lipids was based on LC retention time, mass accuracy (<5 ppm) and detailed structural information inferred from MS/MS data [ 35 ]. Supplementary Tables S2 and S3 show the collected data forming the lipid profile of extracts extracted from Nannochloropsis oceanica and Chlorococcum amblystomatis .…”

Section: Methodsmentioning

confidence: 99%

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Comparison of the Regenerative Metabolic Efficiency of Lipid Extracts from Microalgae Nannochloropsis oceanica and Chlorococcum amblystomatis on Fibroblasts

Stasiewicz,

Conde,

Domingues

et al. 2024

Antioxidants

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UVA radiation leads to oxidative stress and inflammation in skin cells. Therefore, the aim of this study was to compare the effect of lipid extracts from microalgae Nannochloropsis oceanica (N.o.) (marine) and Chlorococcum amblystomatis (C.a.) (freshwater) on the redox balance and PUFA metabolism in human skin fibroblasts modified by UVA. Lipid extracts from both types of microalgae introduced into the fibroblast medium after UVA irradiation significantly reduced the level of ROS and enhanced expression of Nrf2, which increased the activity/level of antioxidants (SOD1/2, CAT, GSH, Trx). The reduction in oxidative stress was accompanied by a decrease in the level of 4-HNE, its protein adducts and protein carbonyl groups. Microalgae also reduced the activity of COX1/2, FAAH and MAGL increased by UVA, and as a consequence, the level of lipid mediators (especially after N.o.) decreased, both from the group of endocannabinoids (AEA, 2-AG, PEA) and eicosanoids (PGE2, 15d-PGJ2, TXB2, 15-HETE), acting mainly through receptors related to G protein, the expression of which increases after UVA. This further contributed to the reduction in oxidative stress and pro-inflammatory signaling at NF-κB and TNFα levels. Therefore, it is suggested that lipid extracts from both N.o. and C.a. microalgae can be used to regenerate fibroblast metabolism disturbed by UVA radiation.

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“…Microalgae synthesize fatty acids mainly for esterification into glycerol-based membrane lipids but n-3 PUFAs can also be esterified to polar lipids such as phospholipids and glycolipids, which are more bioavailable [ 34 ]. These polar lipids can be abundant in the biomass of microalgae as Nannochloropsis [ 35 , 36 ] and Tetraselmis [ 37 ]. The Nannochloropsis sp.…”

Section: Nutritional Value Of Tetraselmis and ...mentioning

confidence: 99%

Bioactivity and Digestibility of Microalgae Tetraselmis sp. and Nannochloropsis sp. as Basis of Their Potential as Novel Functional Foods

et al. 2023

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It is estimated that by 2050, the world’s population will exceed 10 billion people, which will lead to a deterioration in global food security. To avoid aggravating this problem, FAO and WHO have recommended dietary changes to reduce the intake of animal calories and increase the consumption of sustainable, nutrient-rich, and calorie-efficient products. Moreover, due to the worldwide rising incidence of non-communicable diseases and the demonstrated impact of diet on the risk of these disorders, the current established food pattern is focused on the consumption of foods that have functionality for health. Among promising sources of functional foods, microalgae are gaining worldwide attention because of their richness in high-value compounds with potential health benefits. However, despite the great opportunities to exploit microalgae in functional food industry, their use remains limited by challenges related to species diversity and variations in cultivation factors, changes in functional composition during extraction procedures, and limited evidence on the safety and bioavailability of microalgae bioactives. The aim of this review is to provide an updated and comprehensive discussion on the nutritional value, biological effects, and digestibility of two microalgae genera, Tetraselmis and Nannochloropsis, as basis of their potential as ingredients for the development of functional foods.

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Effects of outdoor and indoor cultivation on the polar lipid composition and antioxidant activity of Nannochloropsis oceanica and Nannochloropsis limnetica: A lipidomics perspective

Cited by 22 publications

References 69 publications

Differences and Similarities in Lipid Composition, Nutritional Value, and Bioactive Potential of Four Edible Chlorella vulgaris Strains

Differences and Similarities in Lipid Composition, Nutritional Value, and Bioactive Potential of Four Edible Chlorella vulgaris Strains

Comparison of the Regenerative Metabolic Efficiency of Lipid Extracts from Microalgae Nannochloropsis oceanica and Chlorococcum amblystomatis on Fibroblasts

Bioactivity and Digestibility of Microalgae Tetraselmis sp. and Nannochloropsis sp. as Basis of Their Potential as Novel Functional Foods

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