Effects of oxidants and antioxidants on proliferation of endometrial stromal cells

Foyouzi, Nastaran; Berkkanoğlu, Murat; Arıcı, Aydın; Kwintkiewicz, Jakub; Izquierdo, D. Martínez; Dulęba, Antoni J.

doi:10.1016/j.fertnstert.2004.02.133

Cited by 83 publications

(55 citation statements)

References 15 publications

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“…22 Even if accumulating evidence indicates that oxidative stress is related to the progression of endometriosis, the serum and peritoneal fluid IMA levels of cases at stage 3 and 4 endometriosis were found to be similar in the presented study.…”

Section: Discussioncontrasting

confidence: 54%

Serum and peritoneal fluid levels of ischemia modified albumin in moderate/severe endometriosis

Gök

Taşçı

Dilbaz

et al. 2017

Int J Reprod Contracept Obstet Gynecol

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Background: Recently, the role of oxidative stress in progression of endometriosis has been reported. Ischemia-modified albumin (IMA) is a marker of protein oxidation and very limited number of studies has evaluated the role of IMA in endometriosis. This study was designed to evaluate the serum and peritoneal fluid IMA levels in moderate/severe endometriosis as a marker for oxidative stress.Methods: This study was designed as a prospective controlled clinical trial. The study group consisted of 35 cases who underwent laparoscopy and with a diagnosis of moderate/severe endometriosis. The control group (n=35) was cases without endometriosis that underwent laparoscopy for tubal sterilization. The serum and peritoneal fluid IMA levels were measured spectrophotometrically by colorimetric method with complex of albumin non-binding cobalt and dithioerthreitol.Results: Although the median serum IMA levels in study and control groups were similar (p=0.553), the levels of peritoneal fluid IMA were significantly higher in study group (p=0.044). In endometriosis cases with dysmenorrhea peritoneal fluid IMA levels were much higher than cases without dysmenorrhea (p=0.018).Conclusions: The increased levels of IMA in peritoneal fluid of endometriosis support the possible role of oxidative stress in endometriosis. With this study, peritoneal fluid IMA levels are initially documented in endometriosis cases.

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Section: Discussioncontrasting

confidence: 54%

Serum and peritoneal fluid levels of ischemia modified albumin in moderate/severe endometriosis

Gök

Taşçı

Dilbaz

et al. 2017

Int J Reprod Contracept Obstet Gynecol

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“…Foyouzi et al [1] proved that different reactive oxygen species may modulate the growth of endometrial tissue. Pro-oxidant-antioxidant imbalance may contribute to the development of excessive growth of endometrial stromal cells in endometriosis.…”

Section: Introductionmentioning

confidence: 99%

Disrupted iron metabolism in peritoneal fluid may induce oxidative stress in the peritoneal cavity of women with endometriosis

Polak

Barczyński

Wertel

et al. 2018

Ann Agric Environ Med.

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Introduction and objective. Data on the possible role of peritoneal fluid free radical-mediated oxidative damage in the pathogenesis of endometriosis still remains inconsistent. The aim of the study was to determine iron metabolism markers and their influence on oxidative stress parameters in the peritoneal fluid of women with endometriosis. Materials and method. 110 women with endometriosis and 119 patients with benign ovarian cysts were included in the study. All visible peritoneal fluid was aspirated during laparoscopy from the anterior and posterior cul-de-sacs. under direct vision to avoid blood contamination. Haemoglobin, iron, total oxidative status, and total antioxidant status were measured using standard colourimetric kits. Results. Haemoglobin, iron levels, as well as total oxidative status values were significantly higher, whereas total antioxidant status values were significantly lower in the peritoneal fluid of patients with endometriosis, in comparison to the reference groups. No differences were observed in peritoneal fluid concentrations of all parameters measured in relation to the phase of the menstrual cycle. Conclusions. Peritoneal fluid of women with endometriosis is characterized by disrupted iron metabolism. This is most likely related to an increased number of erythrocytes in the peritoneal cavity of endometriotic women, which leads to a higher concentration of haemoglobin in this environment. Impaired iron homeostasis may have a significant influence on the pathophysiology of peritoneal endometriosis by the direct impact of haemoglobin derivatives and/or formation of the pro-inflammatory and pro-oxidative environment. Peritoneal cavity oxidative stress occurs predominantly in women in advanced stages of the disease.

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“…Induction was performed for 48 hours, and then the cell viability and level of ROS were analyzed. 14 The viable cells were manually counted using a slideimproved Neubauer Hemocytometer under microscope with ×40 magnitude. Afterward, 500 µL of trypsin (1 mg/mL) was added to release the cells from the wells.…”

Section: H 2 O 2 Induction and Viable Cell Countingmentioning

confidence: 99%

Expression is Down-Regulated by Oxidative Stress in Endometriosis and Endometriosis-Associated Ovarian Cancer

2017

Translational Oncogenomics

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Oxidative stress is considered an important factor in the development of endometriosis, including its malignant transformation. Previous studies have found that AT-rich interactive domain 1A (ARID1A), a tumor suppressor gene, is frequently mutated and inactivated in endometriosis-associated ovarian cancer (EAOC), and such a change in this gene is considered an early event in malignant transformation. We observed oxidative stress status by measuring the activity of the antioxidant enzyme manganese superoxide dismutase (MnSOD), malondialdehyde (MDA), and ARID1A gene expression in tissue samples from patients with endometriosis, EAOC, or non-endometriosisassociated ovarian cancer (non-EAOC). We also induced oxidative stress in the cultured cells from patients with primary endometriosis by adding H 2 O 2 and tested for any alteration of ARID1A gene expression based on different H 2 O 2 concentrations. The results showed that MnSOD activity in endometriosis and EAOC was lower than in non-EAOC, but MDA levels were higher. This study also showed that oxidative stress reduced ARID1A gene expression.

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Effects of oxidants and antioxidants on proliferation of endometrial stromal cells

Cited by 83 publications

References 15 publications

Serum and peritoneal fluid levels of ischemia modified albumin in moderate/severe endometriosis

Serum and peritoneal fluid levels of ischemia modified albumin in moderate/severe endometriosis

Disrupted iron metabolism in peritoneal fluid may induce oxidative stress in the peritoneal cavity of women with endometriosis

Expression is Down-Regulated by Oxidative Stress in Endometriosis and Endometriosis-Associated Ovarian Cancer

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