Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol

Abstract

The principal objective of this study was to evaluate the kinetics of dihydroxyacetone production by Gluconobacter frateurii CGMCC 5397 under different oxygen volumetric mass transfer coefficient (kLa) conditions in submerged bioreactors using biodiesel-derived crude glycerol as the carbon source. kLa is a key fermentation parameter for the production of dihydroxyacetone. Cultivations were conducted in baffled- and unbaffled-flask cultures (the kLa values were 24.32 h−1 and 52.05 h−1, respectively) and fed-batch cultures (the kLa values were held at 18.21 h−1, 46.03 h−1, and 82.14 h−1) to achieve high dihydroxyacetone concentration and productivity. The results showed that a high kLa could dramatically increase dihydroxyacetone concentrations and productivities. The baffled-flask culture (with a kLa of 52.05 h−1) favored glycerol utilization and dihydroxyacetone production, and a dihydroxyacetone concentration as high as 131.16 g/L was achieved. When the kLa was set to 82.14 h−1 in the fed-batch culture, the dihydroxyacetone concentration, productivity and yield were 175.44 g/L, 7.96 g/L/h and 0.89 g/g, respectively, all of which were significantly higher than those in previous studies and will benefit dihydroxyacetone industrial production.

Low-cost biotransformation of glycerol to 1,3-dihydroxyacetone throughGluconobacter frateuriiin medium with inorganic salts only

“…1d) were obtained in the media containing 30Á00 g l À1 of glycerol, 3Á10 g l À1 of (NH 4 ) 2 SO 4 , 1Á67 g l À1 of K 2 HPO 4 , 1Á31 g l À1 of KH 2 PO 4 , 6Á00 g l À1 of CaCO 3 , and 0Á38 g l À1 of MgSO 4 Á7H 2 O. Note that the DHA-Prod obtained in a simple flask batch culture of this study may be further enhanced by a fed-batch bioreactor system developed in other studies (Hu et al 2011;Liu et al 2013;Zheng et al 2016).…”

“…Gluconobacter frateurii has also been used for producing DHA in a culture medium containing glycerol and supplemented with yeast extract and CaCO 3 . The yield obtained through this method was around 73-89% (Liu et al 2013;Zheng et al 2016).…”

“…The biotransformations of glycerol into DHA have been performed using a few species of acetic acid bacteria including Acetobacter (Virtanen and Nordlund 1933;Nabe et al 1979) and Gluconobacter (Flickinger and Perlman 1979;Ohrem and Voß 1996;Hekmat et al 2007;Hu et al 2010Hu et al , 2011Hu et al , 2012Hu et al , 2017Ma et al 2010;Liu et al 2013;Zheng et al 2016;Zhou et al 2016). The culture media containing glycerol as a carbon source and yeast extract as supplemental nutrients were used for culturing Acetobacter suboxydans (Virtanen and Nordlund 1933), Acetobacter xylinum (Nabe et al 1979) and Gluconobacter oxydans (Hu et al 2010(Hu et al , 2011(Hu et al , 2012(Hu et al , 2017Ma et al 2010;Zhou et al 2016).…”

Biorefinery for Glycerol Rich Biodiesel Industry Waste

“…Gluconobacter strains are extensively used to produce DHA via the incomplete oxidation of glycerol with the help of glycerol dehydrogenases. The efficiency of DHA yield in fed-batch culture by Gluconobacter frateurii was enhanced by high oxygen transfer coefficient of 82.14/ h to 0.89 g/g [97].…”

Kinetic Modeling of Dihydroxyacetone Production from Glycerol by Gluconobacter oxydans ATCC 621 Resting Cells: Effect of Fluid Dynamics Conditions

“…The most widely employed microorganism to produce DHA from glycerol as raw material is Gluconobacter oxydans, although there are several different works using other microorganisms [5][6][7][8][9][10][11][12][13][14][15][16]. G. oxydans is a Gram-negative aerobic bacterium, whose respiratory metabolism allows it to partially oxidize different organic compounds [17].…”