Effects of ozone (O3) therapy on cisplatin-induced ototoxicity in rats

Abstract

The aim of this study is to investigate the effect of rectal ozone and intratympanic ozone therapy on cisplatin-induced ototoxicity in rats. Eighteen female Wistar albino rats were included in our study. External auditory canal and tympanic membrane examinations were normal in all rats. The rats were randomly divided into three groups. Initially, all the rats were tested with distortion product otoacoustic emissions (DPOAE), and emissions were measured normally. All rats were injected with 5-mg/kg/day cisplatin for 3 days intraperitoneally. Ototoxicy had developed in all rats, as confirmed with DPOAE after 1 week. Rectal and intratympanic ozone therapy group was Group 1. No treatment was administered for the rats in Group 2 as the control group. The rats in Group 3 were treated with rectal ozone. All the rats were tested with DPOAE under general anesthesia, and all were sacrificed for pathological examination 1 week after ozone administration. Their cochleas were removed. The outer hair cell damage and stria vascularis damage were examined. In the statistical analysis conducted, a statistically significant difference between Group 1 and Group 2 was observed in all frequencies according to the DPOAE test. In addition, between Group 2 and Group 3, a statistically significant difference was observed in the DPOAE test. However, a statistically significant difference was not observed between Group 1 and Group 3 according to the DPOAE test. According to histopathological scoring, the outer hair cell damage score was statistically significantly high in Group 2 compared with Group 1. In addition, the outer hair cell damage score was also statistically significantly high in Group 2 compared with Group 3. Outer hair cell damage scores were low in Group 1 and Group 3, but there was no statistically significant difference between these groups. There was no statistically significant difference between the groups in terms of stria vascularis damage score examinations. Systemic ozone gas therapy is effective in the treatment of cell damage in cisplatin-induced ototoxicity. The intratympanic administration of ozone gas does not have any additional advantage over the rectal administration.

Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders

“… Histopathological scoring: decreased stria vascularis damage, decreased inner–outer hair cell damage. ( Koçak et al, 2016 ) Postconditioning : 30 μg/ml, intravenous, daily administration for 14 days, at the same time with noise exposure. Reduction: malondialdehyde (MDA), % mitochondrial swelling, mitochondrial membrane potential (MMP), Glutathione disulfide (GSSG), cytochrome c (Brain, cochlear).…”

Modulation of Oxidative Stress by Ozone Therapy in the Prevention and Treatment of Chemotherapy-Induced Toxicity: Review and Prospects

“…Finally, in 2016, Kocak et al [52] published a different experimental work, evaluating the effect of O 3 /O 2 in the management of already established cisplatin-induced ototoxicity. Rats were treated with intratympanic and rectal ozone one session/days for 7 days.…”

Modulation by Ozone Therapy of Oxidative Stress in Chemotherapy-Induced Peripheral Neuropathy: The Background for a Randomized Clinical Trial

“…Ototoxicity was significantly lower (p < 0.05) in ozone groups than the control group, with (1) partial recovery of audition and lower distortion of product otoacoustic emissions, and (2) lower histopathological damage in the outer hair cell of the inner ears. However, the addition of intratympanic insufflation did not provide further benefit when compared with rectal insufflation of ozone alone [79].…”