Effects of paralytic shellfish toxins on the middle intestine of Oncorhynchus mykiss: Glutathione metabolism, oxidative status, lysosomal function and ATP-binding cassette class C (ABCC) proteins activity

Painefilú, Julio C.; Bianchi, Virginia Angélica; Krock, Bernd; Anna, Julieta S. De; Kristoff, Gisela; Luquet, Carlos Marcelo

doi:10.1016/j.ecoenv.2020.111069

Cited by 13 publications

(7 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro and ex vivo experiments in rainbow trout and the Patagonian silverside (Odontesthes hatcheri) suggest that microcystin-LR may act as a substrate and competitive inhibitor of ABCClike proteins in intestinal tissues (Bieczynski et al, 2014(Bieczynski et al, , 2016. Additional ex vivo and in vitro experiments in rainbow trout intestines demonstrated that paralytic shellfish toxin (PST) is capable of inhibiting ABCC-like transporters when absorbed by intestinal epithelial cells (Painefilú et al, 2020). The activity of ABC proteins can also be regulated by endogenous compounds.…”

Section: Molecular Interactions Of Xenobiotics With Mxr Transportersmentioning

confidence: 99%

Interactions of Environmental Chemicals and Natural Products With ABC and SLC Transporters in the Digestive System of Aquatic Organisms

Romersi

Nicklisch

2022

Front. Physiol.

View full text Add to dashboard Cite

An organism’s diet is a major route of exposure to both beneficial nutrients and toxic environmental chemicals and natural products. The uptake of dietary xenobiotics in the intestine is prevented by transporters of the Solute Carrier (SLC) and ATP Binding Cassette (ABC) family. Several environmental chemicals and natural toxins have been identified to induce expression of these defense transporters in fish and aquatic invertebrates, indicating that they are substrates and can be eliminated. However, certain environmental chemicals, termed Transporter-Interfering Chemicals or TICs, have recently been shown to bind to and inhibit fish and mammalian P-glycoprotein (ABCB1), thereby sensitizing cells to toxic chemical accumulation. If and to what extent other xenobiotic defense or nutrient uptake transporters can also be inhibited by dietary TICs is still unknown. To date, most chemical-transporter interaction studies in aquatic organisms have focused on ABC-type transporters, while molecular interactions of xenobiotics with SLC-type transporters are poorly understood. In this perspective, we summarize current advances in the identification, localization, and functional analysis of protective MXR transporters and nutrient uptake systems in the digestive system of fish and aquatic invertebrates. We collate the existing literature data on chemically induced transporter gene expression and summarize the molecular interactions of xenobiotics with these transport systems. Our review emphasizes the need for standardized assays in a broader panel of commercially important fish and seafood species to better evaluate the effects of TIC and other xenobiotic interactions with physiological substrates and MXR transporters across the aquatic ecosystem and predict possible transfer to humans through consumption.

show abstract

Section: Molecular Interactions Of Xenobiotics With Mxr Transportersmentioning

confidence: 99%

Interactions of Environmental Chemicals and Natural Products With ABC and SLC Transporters in the Digestive System of Aquatic Organisms

Romersi

Nicklisch

2022

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…In fish, the studies about the ABCC protective function against toxic substances concern mostly Abcc1 and Abcc2 but also Abcc3 to 5 ( Klobučar et al, 2010 ; Cárcamo et al, 2011 ; Long et al, 2011a , b ; Bieczynski et al, 2014 , 2016 ; Painefilú et al, 2019 , 2020 ; Lu et al, 2021 ; among others). Abcc6 is involved in the regulation of tissue calcification, e.g., scales mineralization ( Parreira et al, 2018 ; Sun et al, 2021 ).…”

Section: Abc Proteinsmentioning

confidence: 99%

“…These toxins inhibit basolateral but not apical Abcc-mediated transport. However, the coexposure with the Abcc inhibitor MK571 does not increase the cytotoxic effects of PST, suggesting that these toxins are not Abcc substrates but non-competitive inhibitors ( Painefilú et al, 2020 ).…”

Section: Toxicological Studiesmentioning

confidence: 99%

Expression and Function of ABC Proteins in Fish Intestine

et al. 2021

Self Cite

View full text Add to dashboard Cite

In fish, the intestine is fundamental for digestion, nutrient absorption, and other functions like osmoregulation, acid-base balance, and excretion of some metabolic products. These functions require a large exchange surface area, which, in turn, favors the absorption of natural and anthropogenic foreign substances (xenobiotics) either dissolved in water or contained in the food. According to their chemical nature, nutrients, ions, and water may cross the intestine epithelium cells’ apical and basolateral membranes by passive diffusion or through a wide array of transport proteins and also through endocytosis and exocytosis. In the same way, xenobiotics can cross this barrier by passive diffusion or taking advantage of proteins that transport physiological substrates. The entry of toxic substances is counterbalanced by an active efflux transport mediated by diverse membrane proteins, including the ATP binding cassette (ABC) proteins. Recent advances in structure, molecular properties, and functional studies have shed light on the importance of these proteins in cellular and organismal homeostasis. There is abundant literature on mammalian ABC proteins, while the studies on ABC functions in fish have mainly focused on the liver and, to a minor degree, on the kidney and other organs. Despite their critical importance in normal physiology and as a barrier to prevent xenobiotics incorporation, fish intestine’s ABC transporters have received much less attention. All the ABC subfamilies are present in the fish intestine, although their functionality is still scarcely studied. For example, there are few studies of ABC-mediated transport made with polarized intestinal preparations. Thus, only a few works discriminate apical from basolateral transport activity. We briefly describe the main functions of each ABC subfamily reported for mammals and other fish organs to help understand their roles in the fish intestine. Our study considers immunohistochemical, histological, biochemical, molecular, physiological, and toxicological aspects of fish intestinal ABC proteins. We focus on the most extensively studied fish ABC proteins (subfamilies ABCB, ABCC, and ABCG), considering their apical or basolateral location and distribution along the intestine. We also discuss the implication of fish intestinal ABC proteins in the transport of physiological substrates and aquatic pollutants, such as pesticides, cyanotoxins, metals, hydrocarbons, and pharmaceutical products.

show abstract

“…In the starfish Pisaster ochraceus, STXs inhibited fertilisation and decreased the ability of the starfish to attach to a substrate [60], with STXs also causing mysid mortality and larval abnormalities in sea urchins [79]. STXs have also elicited negative responses in marine fish, causing neurological symptoms and mortality in multiple species [40,42] and a range of effects on rainbow trout intestinal cells [103]. STXs have also caused altered grazing strategies and reduced reproducibility in some copepods [104,105].…”

Section: Sunstar Physiological Analysismentioning

confidence: 99%

The Common Sunstar Crossaster papposus—A Neurotoxic Starfish

et al. 2021

View full text Add to dashboard Cite

Saxitoxins (STXs) are a family of potent neurotoxins produced naturally by certain species of phytoplankton and cyanobacteria which are extremely toxic to mammalian nervous systems. The accumulation of STXs in bivalve molluscs can significantly impact animal and human health. Recent work conducted in the North Sea highlighted the widespread presence of various saxitoxins in a range of benthic organisms, with the common sunstar (Crossaster papposus) demonstrating high concentrations of saxitoxins. In this study, an extensive sampling program was undertaken across multiple seas surrounding the UK, with 146 starfish and 5 brittlestars of multiple species analysed for STXs. All the common sunstars analysed (n > 70) contained quantifiable levels of STXs, with the total concentrations ranging from 99 to 11,245 µg STX eq/kg. The common sunstars were statistically different in terms of toxin loading to all the other starfish species tested. Two distinct toxic profiles were observed in sunstars, a decarbomylsaxitoxin (dcSTX)-dominant profile which encompassed samples from most of the UK coast and an STX and gonyautoxin2 (GTX2) profile from the North Yorkshire coast of England. Compartmentalisation studies demonstrated that the female gonads exhibited the highest toxin concentrations of all the individual organs tested, with concentrations >40,000 µg STX eq/kg in one sample. All the sunstars, male or female, exhibited the presence of STXs in the skin, digestive glands and gonads. This study highlights that the common sunstar ubiquitously contains STXs, independent of the geographical location around the UK and often at concentrations many times higher than the current regulatory limits for STXs in molluscs; therefore, the common sunstar should be considered toxic hereafter.

show abstract

Effects of paralytic shellfish toxins on the middle intestine of Oncorhynchus mykiss: Glutathione metabolism, oxidative status, lysosomal function and ATP-binding cassette class C (ABCC) proteins activity

Cited by 13 publications

References 78 publications

Interactions of Environmental Chemicals and Natural Products With ABC and SLC Transporters in the Digestive System of Aquatic Organisms

Interactions of Environmental Chemicals and Natural Products With ABC and SLC Transporters in the Digestive System of Aquatic Organisms

Expression and Function of ABC Proteins in Fish Intestine

The Common Sunstar Crossaster papposus—A Neurotoxic Starfish

Contact Info

Product

Resources

About