Effects of peroxisome proliferators and/or hypothyroidism on xenobiotic-metabolizing enzymes in rat testis

Mehrotra, Kalika; Morgenstern, Ralf; Lundqvist, Gerd; Becedas, Luisa; Ahlberg, Margot Bengtsson; Georgellis, Antonis

doi:10.1016/s0009-2797(97)00020-3

Cited by 21 publications

(9 citation statements)

References 50 publications

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“…8 Despite the limited studies have been conducted, ASA was shown to cause a dramatic reduction in CAT activity in a study related to the effect of ASA on antioxidant enzyme activities in the testes of rats. 45 In the current study, in the testicular tissue, we found that ASA induced a significant increase in MDA levels, indicating oxidative stress on lipid layers, and significantly decrease in CAT levels. Although melatonin given in order to ameliorate ASA toxicity reduced MDA levels, the reduction was not statistically significant.…”

Section: Discussionsupporting

confidence: 59%

The effect of melatonin on acetylsalicylic acid-induced kidney and testis damage

et al. 2013

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The aim of this study was to evaluate the acute effect of high-dose acetylsalicylic acid (ASA) on kidney and testis, and the potential protective and therapeutic effects of melatonin on ASA-related pathology. A total of 40 rats were randomly divided into the following 5 groups ( n = 8): group 1: control, not given any drug; group 2: only 200 mg/kg ASA was given; group 3: 5 mg/kg melatonin was given 45 min before administering 200 mg/kg ASA; group 4: 5 mg/kg melatonin was given 45 min after administering 200 mg/kg ASA; and group 5: only 5 mg/kg melatonin was given. The histopathological changes and the biochemical findings; such as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), reduced glutathione (GSH), and blood urea nitrogen (BUN) as well as serum creatinine (Cr) levels were evaluated. ASA significantly increased MDA levels in both kidney and testis, whereas it significantly decreased the values of SOD, CAT, GPX, and GSH in kidney and CAT levels in testis. Melatonin significantly decreased MDA levels in kidney and ameliorated it in testis, whereas it caused elevation in the levels of antioxidants. BUN and Cr levels were higher after ASA, whereas these levels were diminished after melatonin administration. The improvement obtained by melatonin on ASA-induced histological alterations was more prominent when it was used after ASA in kidney and before ASA in testis. In this study, we demonstrated the beneficial effect of melatonin on high-dose ASA-related pathology of kidney and testis for the first time.

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Section: Discussionsupporting

confidence: 59%

The effect of melatonin on acetylsalicylic acid-induced kidney and testis damage

et al. 2013

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“…The effect of dietary broccoli is low in rat testes compared with the activities of phase I enzyme activities in liver and intestine (Vang et al 2000, submitted). Induction of the described testicular enzyme activities in a parallel experiment (11) shows that the present testes-model works well.…”

Section: Resultssupporting

confidence: 57%

Effects of dietary broccoli on rat testicular xenobiotic metabolizing enzymes

Vang

Mehrota

Georgellis

et al. 1999

Eur. J. Drug Metab. Pharmacokinet.

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The effects of vegetables on the activities of various metabolizing enzymes in liver and intestine have been studied intensively, whereas studies on effects on testicular metabolizing enzymes are lacking. The present report is the first describing the effects of dietary broccoli on the activities of a number of xenobiotic metabolizing enzymes from rat testes. Groups of male Wistar rats were fed a semisynthetic diet with 10% (w/w) freeze-dried broccoli for 1 week. Different broccoli samples with varying content of glucosinolates were used. Dietary broccoli significantly increased the activities of two testicular phase II enzymes--glutathione S-transferase (1.6-fold) and UDP-glucuronosyl transferase (1.8-fold). The activities of these enzymes differed significantly depending on the conditions during cultivation of the broccoli, because of differences in the content of glucosinolates and other secondary plant metabolites. The levels of two glutathione S-transferase subunits, rGSTM2 and rGSTA, were determined using Western blotting analysis and the levels of both subunits were reduced in animals fed broccoli grown at low S-fertilizer level. Broccoli did not statistically significantly modulate the activities of the phase I enzymes, epoxide hydrolase or NAD(P)H quinone-oxidoreductase, or the phase II enzyme p-sulphotransferase, or the anti-oxidative enzymes catalase and total glutathione peroxidase in rat testes. In general, dietary broccoli affects phase I and phase II enzyme levels in rat testes much less than found in liver, however, two rat testicular phase II xenobiotic metabolizing enzymes were induced.

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“…The glutathione adducts produced in the detoxification process have an increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted. Recent studies have shown that several phthalates caused a significant decrease in the GST activity in rats and hamsters (57)(58)(59). This inhibitory effect of phthalates in the GST activity permits the modulation of the biological response and toxicity of chemicals that are detoxified or metabolized via GST.…”

Section: Fig 4 Representative 2-de Cy Dye-stained Protein Gels Withmentioning

confidence: 99%

Identification of Proteomic Signatures of Exposure to Marine Pollutants in Mussels (Mytilus edulis)

Apraiz

Cristóbal

2006

Molecular & Cellular Proteomics

163

121

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Bivalves and especially mussels are very good indicators of marine and estuarine pollution, and so they have been widely used in biomonitoring programs all around the world. However, traditional single parameter biomarkers face the problem of high sensitivity to biotic and abiotic factors. In our study, digestive gland peroxisome-enriched fractions of Mytilus edulis (L., 1758) were analyzed by DIGE and MS. We identified several proteomic signatures associated with the exposure to several marine pollutants (diallyl phthalate, PBDE-47, and bisphenol-A). Animals collected from North Atlantic Sea were exposed to the contaminants independently under controlled laboratory conditions. One hundred and eleven spots showed a significant increase or decrease in protein abundance in the two-dimensional electrophoresis maps from the groups exposed to pollutants. We obtained a unique protein expression signature of exposure to each of those chemical compounds. Moreover a set of proteins composed a proteomic signature in common to the three independent exposures. It is remarkable that the principal component analysis of these spots showed a discernible separation between groups, and so did the hierarchical clustering into four classes. The 14 proteins identified by MS participate in ␣-and ␤-oxidation pathways, xenobiotic and amino acid metabolism, cell signaling, oxyradical metabolism, peroxisomal assembly, respiration, and the cytoskeleton. Our results suggest that proteomic signatures could become a valuable tool to monitor the presence of pollutants in field experiments where a mixture of pollutants is often present. Further studies on the identified proteins could provide crucial information to understand possible mechanisms of toxicity of single xenobiotics or mixtures of them in marine ecosystems.

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Effects of peroxisome proliferators and/or hypothyroidism on xenobiotic-metabolizing enzymes in rat testis

Cited by 21 publications

References 50 publications

The effect of melatonin on acetylsalicylic acid-induced kidney and testis damage

The effect of melatonin on acetylsalicylic acid-induced kidney and testis damage

Effects of dietary broccoli on rat testicular xenobiotic metabolizing enzymes

Identification of Proteomic Signatures of Exposure to Marine Pollutants in Mussels (Mytilus edulis)

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