Effects of peroxynitrite and lipopolysaccharide on mitotic activity of C6 glioma cells

Kulahava, Tatsiana; Семенкова, Г. Н.; Kvacheva, Z. B.; Черенкевич, С. Н.; Timoshenko, Alexander V.

doi:10.1016/j.neulet.2006.01.013

Cited by 3 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, Kulahava T.A. et al reported the mitotic effect of 100 µg/ml of LPS dose on C6 glioma cell line, which was approximately 10 fold more than our cytostatic dose 7 . In our study we can conclude that over dose( 4 µg/ml, 8 µg/ml) could be toxic for the cells.In another study that analysedthe effects of LPS on mononuclear phagocytesit has been mentioned both the letal (cytotoxic effects) and the stimulatory effects of LPS 8 .In our study we also observed a mitotic activitywhen 0.25 µg/ml, 0.5 µg/mland 1 µg/ml LPS was added to the cells.…”

Section: Discussioncontrasting

confidence: 66%

Re-evaluation Of LPS Concentrations On The 293T Human Renal Cell Line

Çakıris¹,

Cakar²,

Abacı³

et al. 2016

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi

View full text Add to dashboard Cite

The inflammatory responsiveness of the cells to Lipopolysaccharides (LPS) is commonly used for in vitro experiments. However, the correct dose of the LPS for cell line experiments is elusive. The LPS concentration that gives the maximal response is a critical point of in vitro inflammatory experiments. The aim of this study was to reevaluate the concentration dependent effect of LPS on the 293T human renal cell line. Methods:We evaluated the cell-detachment assay of LPS-stimulated 293T cell line monitored by xCELLigence Real-Time Cell Analyzer (RTCA) system. We applied increasing concentration of LPS followed by Roche xCELLigence Instrument based on the Real-Time Cell Analysis System. Results:Our results demonstrated that the 2, 4 and 8µg of LPS inhibit cell division which diverts cells to a steady-state phase, 1 µg acts as mitogen. Lower concentrations are no effect on cells. Conclusion:These work showed that LPS concentrations had various effects on cell proliferation and need to be estimated for each experiment before carry out the experiments.

show abstract

Section: Discussioncontrasting

confidence: 66%

Re-evaluation Of LPS Concentrations On The 293T Human Renal Cell Line

Çakıris¹,

Cakar²,

Abacı³

et al. 2016

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi

View full text Add to dashboard Cite

show abstract

“…The number of cells in prophase, metaphase, anaphase, and telophase of mitosis was counted in the same manner. 21…”

Section: Assaymentioning

confidence: 99%

“…Cells were counted using Goryaev's chamber by light microscopy method, using microscope Biomed Biooptic B-200 (Biomed-Service, Russia). Proliferation was expressed as proliferative index (number of accumulated cells/number of seeded cells) 21.…”

mentioning

confidence: 99%

2‐Hexadecenal inhibits growth of C6 glioma cells

Семенкова

Kvacheva

Lisovskaya

et al. 2019

Cell Biochemistry & Function

Self Cite

View full text Add to dashboard Cite

2-Hexadecenal (2HD) formation in the organism occurs via irreversible enzymatic degradation of sphingosine-1-phosphate or nonenzymatic γ-, UV-, or HOCl-induced destruction of a number of sphingolipids including S1P. The current research focuses on the study of 2HD effects on C6 glioma cells growth. The results obtained show that 2HD causes a dose-dependent decrease in proliferative and mitotic indices.The change in the mitotic index is due to the redistribution of cells in the different phases of mitosis. These processes are accompanied by cytoskeleton rearrangement and changes in cell morphology, which are expressed in F-actin redistribution, change in the number and type of filopodia and fibrils, leading to cell shape changes, decrease in intercellular contacts and monolayer rarefaction. Cells treatment with 2HD leads to apoptosis induction and signalling pathways modification, including activation of JNK, p38, and ERK1/2 MAPK but not PI3K. The effects observed are not related to the cytotoxicity of 2HD. Significance of the study: 2HD-an unsaturated aldehyde, which level can rise under conditions of oxidative stress as a result of nonenzymatic sphingolipids' destruction. The mechanisms of 2HD action on various cell types have not been sufficiently studied. Therefore, the study on functional role of this aldehyde in different cell types that may be its target is relevant. This study demonstrated that 2HD inhibits growth of C6 glioma cells due to modification of intracellular processes of signal transduction, cytoskeleton rearrangement, change in the mitotic regimen and apoptosis induction.

show abstract

“…Accumulation of these lipid peroxides and their lipid/protein degradation products have been linked to protein aggregation in the nerves and subsequent development of neurodegenerative disorders, such as Parkinson's, 19,20 and glioma. [21][22][23] Furthermore, peroxynitrite-induced lipid oxidation has been linked to the progression of cardiovascular diseases like diabetic cardiomyopathy. 24 As peroxynitrite is emerging to be a key species involved in cellular stress and different forms of pathophysiologies, 13,25,26 developing molecular tools to investigate its impact on biomolecules, including lipids, is of fundamental importance.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct Assessment of Nitrative Stress in Lipid Environments: Applications of a Designer Lipid-Based Biosensor for Peroxynitrite

Gutierrez

Erguven

Stone

et al. 2022

Preprint

View full text Add to dashboard Cite

Peroxynitrite (ONOO−), a redox-active species generated under nitrosative stress conditions, can promote oxidative damage of lipids. Herein, we report the development and multifaceted utilization of a novel, activity-based phospholipid probe, PLP-ONOO–, for imaging lipid environments that are targeted by ONOO− in biomimetic and biological systems. Using PLP-ONOO– and the natural lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, we built synthetic protocell membranes in the form of giant vesicles (GVs). These GVs responded to ONOO− by lighting up and displayed excellent selectivity against other redox-active species, introducing an unprecedented function to biomimetic membrane architectures. Live HeLa cells containing PLP-ONOO– were investigated under cellular nitrosative stress induced by either external administration of peroxynitrite or endogenous stimulation with interferon-γ / lipopolysaccharide / phorbol myristate acetate. Both conditions enhanced the coumarin fluorescence within cells, providing insight into intracellular lipid environments potentially prone to peroxynitrite-mediated oxidative damage. This work offers a chemical reactivity-dependent function for synthetic vesicles and opens a possibility for investigating nitrosative stress at subcellular levels.

show abstract

Effects of peroxynitrite and lipopolysaccharide on mitotic activity of C6 glioma cells

Cited by 3 publications

References 30 publications

Re-evaluation Of LPS Concentrations On The 293T Human Renal Cell Line

Re-evaluation Of LPS Concentrations On The 293T Human Renal Cell Line

2‐Hexadecenal inhibits growth of C6 glioma cells

Direct Assessment of Nitrative Stress in Lipid Environments: Applications of a Designer Lipid-Based Biosensor for Peroxynitrite

Contact Info

Product

Resources

About