Effects of pH and dissolved oxygen on cellulose production by Acetobacter xylinum BRC5 in agitated culture

Hwang, Jung Wook; Yang, Yan; Hwang, Jae Kwan; Pyun, Yu‐Ryang; Kim, Yu Sam

doi:10.1016/s1389-1723(99)80199-6

Cited by 158 publications

(66 citation statements)

References 9 publications

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“…The addition of more than 5% of sugar cause the plasmolysis in these bacterial cells, thus decreasing the formation of cellulose and also resulting in low yield. Table 1 indicates that the optimum pH in this study was pH 5, in accordance with the literature that the optimum pH for A. xylinum growth was pH 5.5 [17]. The thickness of the bacterial cellulose film obtained at pH 5 is 0.072 cm ( Table 1).…”

Section: Yieldsupporting

confidence: 87%

Preliminary study on biosynthesis and characterization of bacteria cellulose films from coconut water

Indrianingsih¹,

Rosyida²,

Jatmiko³

et al. 2017

IOP Conf. Ser.: Earth Environ. Sci.

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Section: Yieldsupporting

confidence: 87%

Preliminary study on biosynthesis and characterization of bacteria cellulose films from coconut water

Indrianingsih¹,

Rosyida²,

Jatmiko³

et al. 2017

IOP Conf. Ser.: Earth Environ. Sci.

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“…For example, Acetobacter xylinum BRC5 (15,21) and IFO 13693 (41) oxidize up to 80 and 72% of the glucose supplied, respectively. This effect has been also observed in G. xylinus (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Influence of Nutritional Factors on the Nature, Yield, and Composition of Exopolysaccharides Produced by Gluconacetobacter xylinus I-2281

Kornmann

Duboc

Marison

et al. 2003

Appl Environ Microbiol

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The influence of substrate composition on the yield, nature, and composition of exopolysaccharides (EPS) produced by the food-grade strain Gluconacetobacter xylinus I-2281 was investigated during controlled cultivations on mixed substrates containing acetate and either glucose, sucrose, or fructose. Enzymatic activity analysis and acid hydrolysis revealed that two EPS, gluconacetan and levan, were produced by G. xylinus. In contrast to other acetic acid strains, no exocellulose formation has been measured. Considerable differences in metabolite yields have been observed with regard to the carbohydrate source. It was shown that glucose was inadequate for EPS production since most of this substrate (0.84 C-mol/C-mol) was oxidized into gluconic acid, 2-ketogluconic acid, and 5-ketogluconic acid. In contrast, sucrose and fructose supported a 0.35 C-mol/C-mol gluconacetan yield. In addition, growing G. xylinus on sucrose produced a 0.07 C-mol/C-mol levan yield. The composition of EPS remained unchanged during the course of the fermentations. Levan sucrase activity was found to be mainly membrane associated. In addition to levan production, an analysis of levan sucrase's activity also explained the formation of glucose oxides during fermentation on sucrose through the release of glucose. The biosynthetic pathway of gluconacetan synthesis has also been explored. Although the activity of key enzymes showed large differences to be a function of the carbon source, the ratio of their activities remained similar from one carbon source to another and corresponded to the ratio of precursor needs as deduced from the gluconacetan composition.

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“…These results are consistent with those of Keshk and Sameshima (2005). This rapid drop in pH is caused by the known conversion of glucose to gluconic acid (Hwang et al 1999). Sucrose induces a similar decrease of the pH, although this effect is less pronounced because it dissociates into glucose and fructose, so the glucose available to convert into gluconic acid is just half the amount.…”

Section: Carbon Source Selectionmentioning

confidence: 99%

The Effect of Carbon and Nitrogen Sources on Bacterial Cellulose Production and Properties from Gluconacetobacter sucrofermentans CECT 7291 Focused on its use in Degraded Paper Restoration

Santos¹,

Carbajo²,

Villar³

2013

BioResources

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Bacterial cellulose (BC) synthesized by Gluconacetobacter sucrofermentans has a high degree of crystallinity, durability, great resistance, and biocompatibility. This study evaluates the effect that carbon and nitrogen sources, present in HS (Hestrin-Schramm) culture medium, have on BC from G. sucrofermentans CECT 7291 used to restore damaged documents. The initial pH of the medium and the temperature were set for all assays, which were conducted in static conditions. The cellulose layers were obtained at four time points. The growth media were characterized at each time point (pH and carbon source consumption). Cellulose layers were washed, pressed, dried, and characterized by determining their pH, dry weight, and optical and mechanical properties. The best combination of carbon and nitrogen sources proved to be fructose plus yeast extract-corn steep liquor, with or without ethanol, which provided a good balance between BC production and carbon source consumption, and generated a resistant and homogeneous cellulose layer.

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Effects of pH and dissolved oxygen on cellulose production by Acetobacter xylinum BRC5 in agitated culture

Cited by 158 publications

References 9 publications

Preliminary study on biosynthesis and characterization of bacteria cellulose films from coconut water

Preliminary study on biosynthesis and characterization of bacteria cellulose films from coconut water

Influence of Nutritional Factors on the Nature, Yield, and Composition of Exopolysaccharides Produced by Gluconacetobacter xylinus I-2281

The Effect of Carbon and Nitrogen Sources on Bacterial Cellulose Production and Properties from Gluconacetobacter sucrofermentans CECT 7291 Focused on its use in Degraded Paper Restoration

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