Preliminary study on biosynthesis and characterization of bacteria cellulose films from coconut water

Indrianingsih, Anastasia Wheni; Rosyida, Vita Taufika; Jatmiko, Tri Hadi; Prasetyo, Dwi Joko; Poeloengasih, Crescentiana Dewi; Apriyana, Wuri; Nisa, Karimatun; Nurhayati, Septi; Hernawan,; Darsih, Cici; Pratiwi, Darmayanti; Suwanto, Andri; Ratih, Dwi

doi:10.1088/1755-1315/101/1/012010

Cited by 16 publications

(7 citation statements)

References 15 publications

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“…The ball burst test was carried out with Instron tensile testing machine equipped with the original test unit (Fig. 1) according to ISO 9073-5:2008 standard [23].…”

Section: Methodsmentioning

confidence: 99%

Influence of Drying Temperature on Tensile and Bursting Strength of Bacterial Cellulose Biofilm

Sederavičiūtė¹,

Domskienė²,

Baltiņa³

2019

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The article presents an experimental study of mechanical properties of cellulose biofilm produced by bacterial fermentation process. Naturally derived biomaterial has great current and potential applications therefore the conditions of material preparation as well as control and prediction of mechanical properties is still a relevant issue. Bacterial cellulose was obtained as a secondary product from Kombucha drink. Presented technique for material preparation and drying is particularly simple and easy to access. The influence of drying temperature (25 °C, 50 °C and 75 °C) on the sample size (thickness and planar dimensions) and mechanical properties (tensile and bursting strength) of cellulose biofilm has been evaluated. It was estimated that during drying biofilm specimens lost up to 92 % of weight and up to 87 % of thickness therefore planar specimen dimensions varied insignificantly. The study showed that the drying temperature is important for optimum strength properties of bacterial cellulose biofilm. The maximum tensile strength (27.91 MPa) was recorded for the samples dried at temperature of 25 °C, when the moisture from the biomaterial is removed gradually and good deformation properties are ensured (respectively tensile extension 18.8 %). Under higher drying temperature biomaterial shows lower values of tensile strength and higher values of bursting strength. The maximum bursting strength (57.2 MPa) was recorded for samples dried at 75 °C when punch displacement changes were insignificant for all tested samples (from 17.8 mm to 21.7 mm). DOI: http://dx.doi.org/10.5755/j01.ms.25.3.20764

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“…The ball burst test was carried out with Instron tensile testing machine equipped with the original test unit (Fig. 1) according to ISO 9073-5:2008 standard [23].…”

Section: Methodsmentioning

confidence: 99%

Influence of Drying Temperature on Tensile and Bursting Strength of Bacterial Cellulose Biofilm

Sederavičiūtė¹,

Domskienė²,

Baltiņa³

2019

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“…Dalam membuat nata de coco industri menambahkan gula sebanyak kurang lebih 1-2% dalam komposisi medium yang digunakan (Budhiono et al, 1999). Namun penambahan gula sebanyak 5% juga dapat meningkatkan pertumbuhan A. xylinum pada medium air kelapa (Indrianingsih et al, 2017). Selain dari air kelapa, A. xylinum juga dapat memproduksi selulosa dengan sumber glukosa dari buah nanas (Malvianie et al, 2014).…”

Section: Penentuan Kondisi Optimum Produksi Bncunclassified

Optimasi Produksi Bacterial Nanocellulose dengan Metode Kultur Agitasi

et al. 2021

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Hampir sebanyak 90% industri farmasi di Indonesia masih menggunakan bahan baku impor. Indonesia memiliki salah satu bahan baku yang cukup melimpah yaitu selulosa. Bacterial nanocellulose (BNC) adalah hasil sintesis dari bakteri aerobic seperti bakteri asam asetat Gluconacetobacter spp. yang berbentuk selulosa murni dengan diameter berukuran nano. Bahan baku BNC yang digunakan dalam industri farmasi adalah BNC dalam bentuk slurry atau high viscose nanocellulose. Tujuan penelitian ini adalah untuk memilih bakteri dan kondisi optimum dalam memproduksi BNC. Bakteri yang digunakan adalah Gluconacetobacter xylinus dan Gluconacetobacter intermedius yang berasal dari InaCC-LIPI dan Gluconacetobacter sp. dari industri nata de coco. Inokulum dari ketiga jenis kultur bakteri tersebut dikultivasi selama 7 hari dalam medium Hestrin&Schramm (HS) cair menggunakan kultur statis dan agitasi dengan kecepatan pengadukan 150 rpm pada pH 5 dan suhu 25 ºC. Isolat bakteri Gluconacetobacter sp. dipilih sebagai bakteri penghasil BNC karena memiliki nilai yield paling tinggi. Kemudian isolat tersebut ditumbuhkan pada variasi kecepatan agitasi (100, 150, dan 200 rpm), variasi pH (4,0; 4,5; 5,0; dan 6,0), dan variasi suhu (25-30 ºC). Penelitian ini menunjukkan bahwa Gluconacetobacter sp. memiliki kondisi optimum pada kecepatan agitasi 150 rpm, pH 5,5, dan suhu 27 ºC. Optimization of Bacterial Nanocellulose Production in Agitation Culture MethodsAbstractAlmost 90% of pharmaceutical industry in Indonesia still uses imported raw material. However, Indonesia has one of the abundant raw materials which is cellulose. Bacterial nanocellulose (BNC) is a pure form of nanocellulose biopolymer material synthesized by microbes such as acetic acid bacteria of Gluconacetobacter spp. as pure cellulose and having diameter in nano scale. BNC used in pharmaceutical industry is in the slurry form/high viscose nanocellulose. The purpose of this study is to determine the bacteria and the optimum conditions to produce BNC. The bacteria used were Gluconacetobacter xylinus and Gluconacetobacter intermedius from InaCC-LIPI and Gluconacetobacter sp. from nata industry. The inoculums were cultivated for 7 days in liquid Hestrin & Schramm (HS) medium using static and agitation culture with a stirring speed of 150 rpm at pH 5 and temperature 25 ºC. The production of BNC has been conducted by using Gluconacetobacter sp., because it has the highest yield. Then it was inoculated at different variation of agitation speed (100, 150, and 200 rpm), pH (4.0; 4.5; 5.0; and 6.0), and temperature (25-30 ºC). This research shows that Gluconacetobacter sp. has optimum conditions at the agitation speed of 150 rpm, pH 5.5, and temperature 27 ºC.Keywords: Bacterial nanocellulose, Gluconacetobacter, agitation

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“…This swelling percentage of Ag-BC was determined using method by Maneerung et al (2007) and Indrianingsih et al (2017) [11, 17a] with little modifications. Ag-BC composites and pure BC as a control medium were cut into a size of 0.5 cm x 0.5 cm in length and immersed in the distilled water for 3 days or 72 hours.…”

Section: Swelling Measurementmentioning

confidence: 99%

NOVEL COMPOSITE OF SILVER-BACTERIAL CELLULOSE (Ag-BC) FROM SIWALAN SAP (Borassus flabellifer) AND ITS ANTIBACTERIAL ACTIVITY

2020

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Recently, a wound healing from natural composite with excellent properties is in a high demand. In this study, a novel composite of bacterial cellulose made from Siwalan sap (Borassus flabellifer) was achieved. Siwalan is a common plant in Java Island of Indonesia and the application is very limited for beverage only. This study aims to determine the effect of the AgNO3/NaBH4 concentration ratio in the development of Ag-BC composites and its antibacterial properties from Siwalan sap. Ag-BC composites were prepared by impregnating the silver solution into the BC matrix through the reduction process with NaBH4. Characterization of Ag-BC composites conducted using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX). Antibacterial assay was performed using disc diffusion method against Salmonella typhimurium (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria. The characterization of Ag-BC composite shows the nanostructure of BC with a length of fiber around 35-60 nm in width. The SEM-EDX micrograph showed that silver particles were impregnated into the BC matrix. Antibacterial activity test results showed that the Ag-BC composite had the ability to inhibit the bacteria S. typhimurium and S. aureus with good inhibition. This result showed the potential application of Ag-BC composite from Siwalan plant as a natural material for medical and pharmaceutical purpose, especially as an antibacterial agent.

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Preliminary study on biosynthesis and characterization of bacteria cellulose films from coconut water

Cited by 16 publications

References 15 publications

Influence of Drying Temperature on Tensile and Bursting Strength of Bacterial Cellulose Biofilm

Influence of Drying Temperature on Tensile and Bursting Strength of Bacterial Cellulose Biofilm

Optimasi Produksi Bacterial Nanocellulose dengan Metode Kultur Agitasi

NOVEL COMPOSITE OF SILVER-BACTERIAL CELLULOSE (Ag-BC) FROM SIWALAN SAP (Borassus flabellifer) AND ITS ANTIBACTERIAL ACTIVITY

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