Effects of phenolic compounds on Agrobacterium vir genes and gene transfer induction—a plausible molecular mechanism of phenol binding protein activation

Joubert, Philippe; Beaupère, D.; Lelièvre, Philippe; Wadouachi, Anne; Sangwan, Rajbir S.; Sangwan‐Norreel, B. S.

doi:10.1016/s0168-9452(02)00012-2

Cited by 43 publications

(24 citation statements)

References 36 publications

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“…Alternatively, wounding may have lead to localized cell necrosis. Joubert et al (2002) reported that certain phenolic compounds inhibited T-DNA transfer and also related this to the general toxicity of phenolics. At 23.3-81.7%, 52.5% on average, we obtained transient GUS expression in marula explants at rates similar to those reported by others.…”

Section: Discussionmentioning

confidence: 99%

Preliminary examination of factors affecting Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birrea subsp. caffra (Anacardiacease)

Mollel¹,

Goyvaerts

2004

Plant Cell, Tissue and Organ Culture

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In planta Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birrea subsp. caffra (Anacardiaceae) resulted in chimeric transgenic in vitro shoots at a rate of 0.8-1.5%. Average transgene expression rates of 33.1 and 1.3% GUS-positive explants were observed on days 3 and 6 after agroinfection, respectively. One to 4 GUS-positive zones were observed per GUS-positive explant section. Addition of acetosyringone (100 lM) during co-cultivation significantly improved transient transformation efficiency as determined by the percentage of GUS-positive explants on day 3 (p < 0.05) whereas wounding did not show a significant effect. However, wounding and acetosyringone acted antagonistically reducing transient transformation rates.

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Section: Discussionmentioning

confidence: 99%

Preliminary examination of factors affecting Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birrea subsp. caffra (Anacardiacease)

Mollel¹,

Goyvaerts

2004

Plant Cell, Tissue and Organ Culture

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“…A number of chemicals may also promote these processes, e.g., acetosyringone (Joubert et al 2002). Ultrasonication can deliver and foreign DNA into protoplasts (Kumar et al 2006).…”

Section: Limitationsmentioning

confidence: 99%

Natural plant genetic engineer Agrobacterium rhizogenes: role of T-DNA in plant secondary metabolism

Chandra

2011

Biotechnol Lett

142

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Agrobacterium rhizogenes is a natural plant genetic engineer. It is a gram-negative soil bacterium that induces hairy root formation. Success has been obtained in exploring the molecular mechanisms of transferred DNA (T-DNA) transfer, interaction with host plant proteins, plant defense signaling and integration to plant genome for successful plant genetic transformation. T-DNA and corresponding expression of rol genes alter morphology and plant host secondary metabolism. During transformation, there is a differential loss of a few T-DNA genes. Loss of a few ORFs drastically affect the growth and morphological patterns of hairy roots, expression pattern of biosynthetic pathway genes and accumulation of specific secondary metabolites.

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“…Not only does the wound site act as an entry point for bacterium but also results in the release of phenolic substances necessary for vir gene activation (Joubert et al 2002). Phenolic compounds, like SA, released by wounded cells have been found to be essential for induction of the virulence genes.…”

Section: Phenolic Compoundsmentioning

confidence: 99%

Agrobacterium-mediated genetic transformation of plants: The role of host

Karami¹,

Esna-Ashari²,

Kurdistani³

et al. 2009

Biologia plant.

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Agrobacterium-mediated genetic transformation is the most widely used technology to obtain overexpression of recombinant proteins in plants. Molecular events that occur within Agrobacterium during interactions with host plants have been studied extensively, and now we have a reasonable understanding the key factors involved in the regulation of T-DNA nuclear import and genomic integration. By contrast, very little is known about the events that take place in the host cells during genetic transformation by Agrobacterium. Here, we describe the plant-related factors including genotype, genes, proteins, competency of target tissues and phenolic compounds that participate in Agrobacteriummediated genetic transformation and discuss their possible roles in this process. Because Agrobacterium probably adapts existing cellular processes for its life cycle, identifying the processes in host cells during Agrobacterium infection might contribute to better understanding of basic biological processes as cell communication, intracellular transport and DNA repair and recombination as well as to expanding the host range of Agrobacterium as a genetic engineering tool.

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Effects of phenolic compounds on Agrobacterium vir genes and gene transfer induction—a plausible molecular mechanism of phenol binding protein activation

Cited by 43 publications

References 36 publications

Preliminary examination of factors affecting Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birrea subsp. caffra (Anacardiacease)

Preliminary examination of factors affecting Agrobacterium tumefaciens-mediated transformation of marula, Sclerocarya birrea subsp. caffra (Anacardiacease)

Natural plant genetic engineer Agrobacterium rhizogenes: role of T-DNA in plant secondary metabolism

Agrobacterium-mediated genetic transformation of plants: The role of host

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